To analyze the resource allocation efficiency and spatial aggregation of elderly care institutions in China, so as to provide reference for optimizing the elderly care service system.

Three-stage DEA model was used to analyze the resource allocation efficiency of elderly care institutions in 31 provinces (autonomous regions and cities) in 2021. Global Moran’s I index and Getis-Ord Gi* index was used to analyze the spatial autocorrelation of resource allocation efficiency of elderly care institutions in China.

In 2021, the overall scale efficiency of resource allocation of elderly care institutions in China was not high, and there was spatial aggregation effect. Scale efficiency was the main factor restricting the improvement of the allocation efficiency of elderly care institutions in China. The resource allocation efficiency of elderly care institutions was greatly affected by the external environment.

According to the demand of local elderly care service, the government should adjust its scale efficiency reasonably, improve the management level, allocate the resources of elderly care institutions reasonably and promote its efficiency.

To analyze and summarize the relationship between sleep quality and dietary inflammatory index (DII), so as to provide guidance for improving sleep quality.

Using dietary inflammatory index, anti-inflammatory diet, anti-inflammatory diet, and sleep quality as keywords, China knowledge net, VIP database, Wan fang database, PubMed and Web of Science database were searched, and the related literatures at home and abroad were reviewed to summarize the relationship between DII and sleep quality and its internal mechanism.

There was a correlation between sleep quality and DII, but the conclusion was not consistent. On one hand, pro-inflammatory or anti-inflammatory diet may affect sleep quality by affecting circadian rhythm system and sleep homeostasis. On the other hand, low-quality sleep may affect dietary selection and intake, thus affecting the level of dietary inflammation.

There is a correlation between sleep quality and DII, but there is no consistent conclusion. Larger samples and high-quality studies are needed in order to provide reference for improving sleep quality.

To explore the causal relationship between lifestyle, diet, sleep, socioeconomic status and bronchial asthma by using two-sample Mendelian randomized method.

Two-sample Mendelian randomization analysis was performed based on inverse variance weighting method (IVW), and the stability and reliability of the results were verified by leaving-one-out method, heterogeneity test, and pleiotropy test.

The results of IVW model showed that smoking (OR=1.17,95%CI: 1.08-1.28), drinking (OR=1.22, 95%CI: 1.05-1.41), sedentary behavior (OR=1.78, 95%CI: 1.38-2.31), sleep duration (OR=2.29, 95%CI: 1.38-2.31), and body mass index (OR=1.41, 95%CI: 1.29-1.53) were associated with the increased risk of bronchial asthma. Fresh fruit intake (OR=0.50, 95%CI: 0.34-0.73), dried fruit intake (OR=0.54, 95%CI: 0.34-0.73), cereal intake (OR=0.62, 95%CI: 0.41-0.94), education level (OR=0.57, 95%CI: 0.34-0.73), and social activity (OR=0.26, 95%CI:0.34-0.73) were associated with reduced risk of asthma. The results of MR-Egger intercept detection did not show pleiotropic effects, indicating that Mendelian randomization was an effective method for causal inference in this study. The heterogeneity test results of drinking (Q=139.92, P=0.001), sedentary behavior (Q=151.37, P=0.003), dried fruit intake (Q=40.00, P=0.007), cereal intake (Q=72.95, P=0.001), and body mass index (BMI) (Q=584.12, P < 0.001) were all statistically significant. Therefore, attention was paid to results of the random effect IVW model.

Smoking, drinking, sedentary behavior, sleep duration, and body mass index may be the risk factors of bronchial asthma. Fresh fruit intake, dried fruit intake, cereal intake, education level, and social activities may be the protective factors of bronchial asthma.

To understand the current status of knowledge and willingness of influenza vaccination among the unvaccinated elderly people in a southwestern city, and to analyze the factors affecting the willingness of influenza vaccination, so as to provide a basis for promoting the popularization of influenza vaccine.

A total of 600 elderly people aged 60 and above who were not vaccinated with influenza vaccine from October to December 2023 were surveyed on their knowledge and willingness to be vaccinated with influenza vaccine. Finally, 538 valid questionnaires were collected. Univariate analysis was carried out using t-test and χ² test, and logistic regression model was used to analyze influenza vaccination intention.

In total 57.8% of the 538 elderly people were willing to be vaccinated against influenza. Specifically, 55.6% of the elderly “did not know or have not heard of the influenza vaccine”, 53.2% of the elderly believed that “the influenza vaccine may not be effective”, 67.5% of the elderly believed that “influenza vaccination would have side effects”, and the estimated proportion of side effects was at 17.53%. The results of multivariate analysis of influenza vaccination willingness showed that people who had “general knowledge” (OR=2.464, 95%CI: 1.209-5.019, P=0.013) and who were “very knowledgeable” (OR=2.885, 95%CI: 1.112-7.485, P=0.029) were more likely to be vaccinated than those who had not heard of influenza vaccine.Compared with the “estimated proportion of influenza vaccination in people aged 60 and over < 20%” group, the “estimated vaccination ratio of 60% to 80%” group (OR=3.478, 95%CI=1.555-7.779, P=0.002), and the “estimated vaccination ratio ≥80%” group (OR=4.591, 95%CI=1.058-19.915, P=0.042) had higher willingness to vaccinate influenza vaccine. The higher the score of satisfaction with personal health, the lower the willingness to be vaccinated against influenza (OR=0.804, 95%CI=0.670-0.964, P=0.018).

The elderly people who have not been vaccinated with influenza vaccine in this area do not know enough about influenza and its vaccine, and the willingness of influenza vaccination is low, which needs to be improved urgently. It is suggested that the knowledge and publicity of influenza and its vaccine be strengthened in this region, and practical vaccination strategies should be adopted to improve the willingness and vaccination rate of influenza vaccination.

To study the drug resistance phenotype, drug resistance gene, and virulence gene of Helicobacter pylori(Hp) in patients with failed eradication therapy, and to provide reference for remedial therapy.

Hp was isolated and cultured from gastric mucosa of patients with failed eradication therapy. Drug sensitivity test was used to detect the resistance of isolated strains to Metronidazole (MTZ), Clarithromycin (CLA), Levofloxacin (LEV), Amoxicillin (AMX), Tetracycline (TET),and Furazolidone (FZ). CLA and LEV resistance genes 23S rRNA, gyrA and virulence genes cagA, vacA, iceA, oipA were amplified by PCR, to further explore the relationship between drug resistance phenotype and drug resistance and virulence genes.

In total 40 strains of Hp were isolated from 58 patients (69.0%). The resistance rates to MTZ, CLA, LEV, AMX, TET, and FZ were 100.0%, 82.5%, 72.5%, 0%, 0%, and 0%, respectively. The most common mutations of 23S rRNA and gyrA genes were T2182C (78.6%) and N87K (32.0%), respectively, and new C2165T and A2219G mutations were found in CLA resistant strains. In addition, the positive rates of virulence genes cagA, vacA s1, vacA s2, vacA m1, vacA m2, iceA1, iceA2, and oipA were 97.1%, 100.0%, 0%, 42.9%, 57.1%, 62.9%, 11.4%, and 68.6%, respectively, and the difference was statistically significant.

The resistance of Hp isolates to MTZ, CLA and LEV in patients with failed eradication therapy in this area is serious, and AMX, TET and FZ are recommended for remedial therapy. The relationship between CLA resistance and C2165T and A2219G mutation and LEV resistance and vacA m2 warrants further investigation.

To explore the relationship among cognitive function, anxiety, and self-management behavior in elderly patients with diabetes mellitus.

A face-to-face survey was conducted among elderly patients with type 2 diabetes mellitus aged 60 and over in the Department of Endocrinology, Penglai People’s Hospital, Yantai city, Shandong Province from October 2021 to May 2022. The Geriatric Anxiety Inventory-Short Form (GAI-SF), the Montreal Cognitive Assessment (MoCA),and self-designed items were used to assess the anxiety, cognitive function, and self-management behaviors of the patients.Path analysis and mediation effect testing were conducted using a structural equation model.

In total 1 319 diabetic patients aged 60 and above were enrolled, of whom 8.3% had anxiety symptoms. The average scores of MoCA scale and self-management behavior were (23.89±4.12) and (3.74±1.27), respectively. Structural equation model analysis showed that cognitive function had a masking effect between anxiety and self-management behavior, and anxiety had a positive effect on self-management behavior directly (β=0.127, P < 0.019). However, it also had a strong negative effect on cognitive function (β=-0.567, P < 0.001) and then had a negative effect on self-management behavior (β=-0.241, P < 0.001). Therefore, anxiety had a negative effect on self-management behavior (β=-0.115, P < 0.001).

The anxiety of elderly patients with diabetes is negatively correlated with their self-management behavior and can have a negative effect on self-management through cognitive function.

To understand the detection of four prohibited veterinary drugs of fresh and live freshwater fish sold in Guangzhou by analyzing the test data from risk monitoring and supervision sampling, so as to provide reference basis for food safety supervision.

Through the Guangzhou Food Safety Risk Monitoring system and the Food Safety Supervision and Inspection Public Information report of Guangzhou Market Administration, the test results of fresh water fish samples collected from supermarkets, markets, and online e-commerce in Guangzhou from 2017 to 2021 were obtained. The detection of nitrofuran drugs, chloramphenicol, sodium pentachloro phenate, and malachite green drugs were analyzed.

The total detection rate of banned veterinary drugs in risk monitoring samples was 35.3% (84/238), and that in supervised sampling samples was 3.10% (118/3 806). The banned veterinary drugs with high detection rates of the two data sources were different. The risk monitoring rates of chloramphenicol and sodium pentachloro phenate were 17.23% (41/238) and 17.23% (41/238), respectively, and that of malachite green was 2.18% (83/3 806). The drug detection rate of the samples collected by risk monitoring in the network e-commerce link was 47.50% (19/40), which was the highest among all sampling links. The results of the two data sources showed that the detection rate of banned veterinary drugs in the wholesale market was high: 47.37% (18/38) and 4.13% (5/121),respectively.

The results of data analysis from the two sources complement each other and jointly reveal the problem of banned veterinary drug residues in fresh freshwater fish sold in Guangzhou from 2017 to 2021. It is suggested that the relevant departments pay more attention to the illegal use of chloramphenicol, sodium pentachloro phenate, and malachite green drugs.

To evaluate the causal relationship between tea drinking and cholelithiasis by two-way Mendelian randomization.

The instrumental variables for tea drinking were determined from 447 485 participants in the UK Biobank genome-wide association study (GWAS). A total of 32 single nucleotide polymorphisms (SNPs) associated with tea drinking were used for two-sample Mendelian randomized analysis to evaluate the causal relationship between tea drinking and cholelithiasis. Cholelithiasis data were obtained from GWAS data of 35 712 cholelithiasis patients and 273 442 controls publicly available from the Finnegan Alliance. In this study, inverse variance weighted (IVW) was used as the main method to estimate causality, and sensitivity analysis was carried out to ensure the robustness of the results. Finally, reverse Mendelian randomization analysis was used to verify whether there was a reverse correlation.

There was a causal relationship between tea drinking and cholelithiasis. In the IVW method, each additional standard deviation (2.85 cups/day) of tea consumption reduced the risk of cholelithiasis by 26.1% (OR=0.739, 95%CI: 0.536-0.990). Similar results were obtained in weighted mode (OR=0.652, 95%CI: 0.458-0.928), but there was no evidence to prove reverse correlation (IVW: P > 0.05).

The genetic evidence provided by two-way Mendelian randomized analysis shows that the increase of tea drinking reduces the risk of cholelithiasis, but there is no evidence of reverse association.

To understand the prevalence and annual change trend of Internet addiction among students aged 11 and 22 in Inner Mongolia in 2019 and 2021, and to explore the influencing factors of Internet addiction behavior, so as to provide reference basis for the prevention of Internet addiction among students.

Students were selected from 12 cities of Inner Mongolia Autonomous Region by stratified cluster random sampling. Internet addiction disorder was determined according to the items of “surveillance and intervention of common diseases and health influencing factors of students in China”. Logistic regression method was used to analyze the relationship between Internet addiction and behavioral factors.

The prevalence of Internet addiction among students aged 11 to 22 years in Inner Mongolia in 2019 was 1.92%, 2.50%, and 2.21%, respectively. Logistic regression analysis showed that vocational high school (OR=1.347, 95%CI: 1.083-1.675), single parent family (OR=1.279, 95%CI:1.090-1.500), reconstituted family (OR=1.326, 95%CI: 1.041-1.690), bullying (OR=1.755, 95%CI: 1.443-2.135), depression (OR=3.557, 95%CI: 3.146-4.021), fighting (OR=3.374, 95%CI: 2.512-4.531), sugary drinks (OR=2.182. 95%CI:1.753-2.716),and eating fried food (OR=2.398, 95%CI: 1.908-3.014) were the risk factor of Internet addiction. Outdoor activities for 2 hours (OR=0.620, 95%CI: 0.530-0.726), non-smoking (OR=0.553, 95%CI: 0.476-0.643), and non-drinking (OR=0.581, 95%CI:0.507-0.666) were the protective factors of Internet addiction.

The current situation of Internet addiction among students in Inner Mongolia is not optimistic. Attention should be paid to bullying, depression, reconstituted families, fighting students, and vocational high school students. Outdoor activities for 1 to 2 hours a day, reducing the intake of sugary drinks and fried foods, and no smoking and drinking can effectively reduce the incidence of Internet addiction.

Tumor is a major public health problem harmful to people’s health. Cell copper death provides a new idea for the basic research of tumor. This paper reviews the latest progress of cell copper death in the field of tumor prevention and treatment.

The literatures related to copper death in tumor prevention and treatment were summarized, and the research status of copper death related genes in different types of tumors was reviewed.

Ferredoxin 1 (FDX1), lipoic acid synthase (LIAS), dihydro lipocyte answers (DLAT), and dihydro lipoamide S-succinyl transferase (DLST) were the key regulatory genes of copper death, which were abnormally expressed in lung cancer, hepatocellular carcinoma, and breast cancer. Among them, the low expression of FDX1 in tumor patients was related to the poor prognosis of the disease, while the overall survival time of cancer patients with high expression of DLAT and DLST genes was decreased. In addition, targeting copper death protein and promoting cell copper death inhibited the proliferation of cancer cells in vivo and in vitro.

Several genes related to copper death, such as FDX1, LIAS, DLAT, and DLST, are abnormally expressed in cancer patients and are related to the occurrence, development, metastasis, and prognosis of the tumor. Targeted promotion of cell copper death and inhibition of cancer cell proliferation provide a new direction for future basic research in the field of tumor, which has public health significance for tumor prevention and treatment.