To construct a Propionibacterium acnes (P. acnes)-induced cellular inflammation model and to study the anti-inflammatory effect of a thermal water.

Utilize human immortalized keratinocytes (HaCaT) was used in the experimentation. Co-incubate the cells with thermal spring water and employ the CCK-8 assay to assess cell viability, screening for the optimal intervention time for the thermal spring water. Expose the cells to varying concentrations of P. acnes for different times, measure cell viability, calculate the 50% inhibitory concentration (IC₅₀) of P. acnes, and determine the model induction conditions by testing IL-6 levels. The cells were soaked in thermal spring water for 0.5 hours, followed by co-culturing with P. acnes as the preventive intervention group, or co-culturing with P. acnes first and then soaking the cells in hot spring water for 0.5 hours as the therapeutic intervention group. The levels of cell viability, pro-inflammatory cytokines, and gene expression of inflammation pathways were assessed using the CCK-8 assay, ELISA, and RT-PCR, respectively. Concurrently, control groups with saline solution, tap water, and pure water, along with model and blank controlswere set up.

After treating the cells with thermal spring water and physiological saline for 0.5 hours, cell viabilities were 92.7% and 99.09%, respectively, which led to the selection of 0.5 hours as the intervention time for subsequent experiments.P. acnes at an OD₆₀₀ of 0.25 was co-incubated with the cells for 5 hours to establish the model. In the preventive intervention, the secretion levels of IL-1β and IL-8， as well as the relative expression levels of IL-6, TNF-α, IL-8, and the genes TLR2 and NF-κB in the cells of the thermal spring water group, were all lower than those in the model group (P<0.05), and significantly lower than those in the saline solution group. In the therapeutic intervention, the contents of IL-6, IL-1β, TNF-α, and IL-8, as well as the expression levels of the genes IL-6, IL-1β, TNF-α, TLR2, and NF-κB in the thermal spring water group, were all lower than those in the model group. The contents of IL-6, IL-1β, TNF-α, and the expression levels of the genes IL-6, IL-1β, IL-8, TLR2, and NF-κB were also significantly reduced compared to those in the saline solution group (P<0.05).

This thermal water may exert its anti-inflammatory effect by down-regulating the expression of TLR2 and NF-κB genes in inflammatory signalling pathway and by reducing the secretion of pro-inflammatory cytokines.

To identify the trajectories of sleep duration among adults in China, and explore the associatedinfluencingfactors.

Data was derived from 2010-2020 China Family Panel Studies (CFPS). Self-reported measures of night sleep duration were used. People aged ≥18 years were included. LCGM and polynomial Logistic regression model were used.

A total of 10 113 adults were included. Four similar trajectory groups of sleep duration were identified among Chinese adults: severely insufficient sleepduration group (3.12%), insufficient sleepduration group (27.63%), moderate sleepduration group (63.41%) and excessive sleepduration group (5.84%). Polynomial logistic regression showed that, using moderate sleep duration group as the reference, individuals who were older (OR=7.29, 95% CI: 2.23-23.83), lived in urban areas (OR=2.45, 95% CI: 1.89-3.17), had higher levels of depressive symptoms (OR=1.12, 95% CI: 1.09-1.15), and people who drank alcohol (OR=1.60, 95% CI: 1.10-2.32) were more likely to be classified into severely insufficient sleep duration group. Males (OR=0.49, 95% CI: 0.35-0.67), living in western regions (OR=0.42, 95% CI: 0.29-0.61) were less likely to be classified into severely insufficient sleep duration group. Older adults (OR=2.38, 95% CI: 2.01-2.83), those with middle school education (OR=1.16, 95% CI: 1.03-1.30), high school education (OR=1.20, 95% CI: 1.04-1.40), college education and above (OR=1.23, 95% CI: 1.01-1.50), living in urban areas (OR=1.34, 95% CI: 1.21-1.48), having more depressive symptoms (OR=1.03, 95% CI: 1.02-1.04), adults drinking alcohol (OR=1.20, 95% CI: 1.05-1.37), and using Internet (OR=1.20, 95% CI: 1.07-1.34) were more likely to be classified into insufficient sleep duration group. Those with higher economic status (OR=0.84, 95% CI: 0.70-1.00), living in western regions (OR=0.51, 95% CI: 0.45-0.58), self-reported fair health (OR=0.86, 95% CI: 0.75-0.98) and good health (OR=0.83, 95% CI: 0.71-0.97), rated life satisfaction as fair (OR=0.74, 95% CI: 0.57-0.94) and satisfied (OR=0.76, 95% CI: 0.60-0.97) were less likely to be in insufficient sleep duration group. Living in the western regions (OR=1.81, 95% CI: 1.44-2.27) were more likely to be classified into excessive sleepduration group, while those aged 30-44 years (OR=0.64, 95% CI: 0.48-0.84), 45-59 years (OR=0.74, 95% CI: 0.55-0.99), having middle school education (OR=0.75, 95% CI: 0.61-0.94), college education and above (OR=0.25, 95% CI: 0.14-0.46), living in urban areas (OR=0.73, 95% CI: 0.60-0.88) were less likely to classified into excessive sleepduration group.

Four groups of sleep duration trajectories among Chinese adults were identified. Sociodemographic factors, socioeconomic status, physical & mental health, health behavior, and life satisfaction were closely related to the trajectories of sleep duration among Chinese adults.

To analyze the characteristics of Adverse Events Following Immunization (AEFI) of domestic rotavirus (live) vaccine (RVVO) in Shaanxi province from 2018 to 2022, and to evaluate the safety of RVVO vaccination.

The AEFI case data on RVVO vaccination in Shaanxi Province between 2018 and 2022 was gathered from the China AEFI Information Management System., and the vaccination situation of RVVO in Shaanxi province was collected through the immunization planning information management system of Shaanxi province. The analysis was conducted using the descriptive epidemiological technique.

A total of 461 102 doses of RVVO were inoculated in Shaanxi province from 2018 to 2022, and 48 cases of AEFI were recorded, with an incidence of 10.41 cases per 100 000 doses, including 43 cases of normal reactions (9.33 cases per 100 000 doses) and 3 cases of abnormal reactions (0.65 cases per 100 000 doses). 2 cases of coupling disease and No serious AEFI cases recorded. The three abnormal reactions were allergic rash (0.22/100 000 doses), acute diarrhea (0.22/100 000 doses) and rotavirus diarrhea (0.22/100 000 doses). 97.92% of the reported AEFI cases were cured or improved after follow-up, and no sequelae and death cases were reported. The age of onset of AEFI was less than 1 year old and occurred on the day of inoculation. The incidence of AEFI at the first, second and third doses was 12.65 cases per 100 000 doses, 10.64 cases per 100,000 doses and 361 cases per 100 000 doses, respectively. RVVO combined with other vaccines reported a general reaction rate of 10.16 per 100 000 doses and an abnormal reaction rate of 0.97 per 100 000 doses in AEFI.

The domestic oral live attenuated rotavirus vaccine has good safety, and the timely vaccination rate of children should be further strengthened.

To examine the relationship between the increase in chronic diseases, self-rated health, and depressive tendencies among middle-aged and older adults in China.

Using data from the 2015 and 2018 waves of CHARLS, this study investigated the mediating role of self-rated health and depressive tendencies in the relationship between the increase in chronic diseases among 12 637 Chinese adults aged 45 and above. The mediation analysis was conducted using the PROCESS 4.0 program and validated through the Bootstrap method.

The prevalence of depressive tendencies among Chinese adults aged 45 and above was 37.11%. After controlling for relevant variables, the increase in chronic diseases significantly impacted both self-rated health and depressive tendencies (c value: t = 30.984, P<0.001; a value: t=17.072, P<0.001). When self-rated health was introduced as a variable, both the increase in chronic diseases and self-rated health significantly affected depressive tendencies (c’ value: t=8.915, P<0.001; b value: t=30.629, P<0.001). Self-rated health partially mediated the relationship between the increase in chronic diseases and depressive tendencies, with the highest effect proportion of 47.74%.

The increase in chronic diseases among middle-aged and older adults can influence depressive tendencies through self-rated health. To reduce depressive tendencies in these patients, it is essential to effectively improve their self-rated health.

To comprehensively assess the disease severity in patients with Wilson’s disease (WD) who had been previously diagnosed and treated with copper removal by Unified Wilson’s Disease Rating Scale (UWDRS) and analyze its influencing factors, in order to provide a reference for patient management and improvement of patients’ health.

Wilson’s disease patients who were admitted to West China Fourth Hospital of Sichuan University from July 2023 to March 2024 were selected as the study subjects, and all patients have been diagnosed according to the diagnostic criteria. The survey was conducted using self-designed structured questionnaires and UWDRS scales. Univariate analysis and multiple linear regression analysis were used to analyze the disease severity and influencing factors of patients with WD who had been previously diagnosed and treated with copper removal.

A total of 103 patients with eligible and complete data were included. The total UWDRS score of patients with WD who underwent copper repulsion therapy was 27.0 (11.5, 60.5), neurological symptom score of 21.0 (4.5, 48.5), hepatic symptom score of 3.0 (1.0, 4.0), and psychiatric symptom score of 5.0 (2.0, 8.0). More than 50% of patients still have symptoms or signs of rapid alternating movements of hand, finger tapping, salivation, gait abnormalities, speech impairment (dysarthria), impaired handwriting, and other bleeding or hematoma. Multiple linear regression analysis showed that education level, classification of first-time diagnosed disease, low-copper diet, social support and medication adherence were the influencing factors of UWDRS total score (P<0.05). The higher the patient’s level of education, medication adherence, and social support are, the lower the UWDRS score is. Patients with hepatic type had lower scores than those with cerebral type, and those who tried to adhere to a low-copper diet had lower scores than those who adhered to a low-copper diet all the time.

More than 50% of the previously diagnosed patients with WD who have been treated with copper removal still have different degrees of neurological symptoms, and the treatment of neurological symptoms needs to be further improved. Education level, classification of first-time diagnosed disease, low-copper diet, social support, and medication adherence were the influencing factors of disease severity in patients with WD.

To evaluate the efficacy and safety of five live attenuated dengue vaccines and one inactivated dengue vaccine using Bayesian network meta-analysis, providing a scientific basis for dengue epidemic prevention and control as well as vaccine development.

A systematic search was conducted in various Chinese and English databases, including PubMed, Cochrane Library, Embase, Web of Science, China National Knowledge Infrastructure (CNKI), and Wan fang Database, based on predefined inclusion and exclusion criteria. Randomized controlled trials (RCTs) assessing the efficacy and safety of six dengue vaccines were selected. The quality of the literature was evaluated using RevMan 5.4 software, and Bayesian network meta-analysis of relevant outcome indicators from the included RCTs was performed using R 4.3.1 software.

A total of 20 studies involving 120 909 participants were included. The results of the Bayesian network meta-analysis indicated that the efficacy of three live attenuated vaccines was notable and the ranking from highest to lowest efficacy was as follows: Butantan-DV > TAK-003 > CYD-TDV. Among these, the dengue vaccine combining serotype 4 non-structural genes and serotype 2 structural genes (Butantan-DV) showed the best preventive effect, with a risk probability of dengue infection post-vaccination lower than that of other vaccines (OR=0.22, 95%CI: 0.09-0.45). In terms of safety, the six vaccines showed varying risks of adverse events and ranked as follows: V180 > CYD-TDV > TV003 > TV005 > Butantan-DV > TAK-003. Notably, the inactivated vaccine (V180) presented a higher risk of adverse events (OR=2.28, 95%CI: 1.02-5.15).

Based on the quantity and quality of the included studies, Butantan-DV exhibited the best efficacy among the three live attenuated vaccines, while CYD-TDV demonstrated the weakest efficacy. TAK-003 showed relatively better safety. The literature on the efficacy of the V180,TV003, and TV005 vaccines is quite limited, suggesting potential directions for future research.

To understand the current state of social isolation among the elderly and its relationship with health-related quality of life, as well as to explore the mediating role of loneliness in this relationship.

A face-to-face questionnaire survey was conducted among 1 349 elderly individuals in Yangzhou using cluster random sampling. Social isolation was measured through three questions regarding social contact, loneliness was assessed using the UCLA Loneliness Scale (short version), and health-related quality of life was evaluated using the EuroQol five-dimensional questionnaire. Propensity score matching (PSM) was employed to match elderly individuals with and without social isolation in a 1:1 ratio without replacement.The mediating effect of loneliness was tested using Monte Carlo simulation analysis.

A total of 718 elderly individuals were included in the study after matching. Social isolation was negatively correlated with health-related quality of life (β=-0.016, 95%CI: -0.030 to -0.002, P<0.05) and positively correlated with loneliness (β=0.142, 95%CI: 0.047 to 0.237, P<0.01). Loneliness acted as a complete mediator in the pathway between social isolation and health-related quality of life among the elderly (β=-0.005, 95%CI: -0.008 to -0.001, P<0.01), with the indirect effect accounting for 31.25% of the total effect.

Social isolation indirectly affects the health-related quality of life of the elderly by increasing their feelings of loneliness. More opportunities for social participation should be provided to the elderly to reduce their loneliness and, consequently, improve their overall quality of life.

To investigate the current status of radiation protection in medical institutions in Sichuan Province, providing a reference for the scientific management and resource optimization of radiation diagnosis and treatment protection within the province.

A questionnaire survey was conducted to analyze medical institutions engaged in radiation diagnosis and treatment activities (excluding dental clinics) across 21 cities (prefectures) in Sichuan Province.

By the end of 2023, there were 3 424 medical institutions in Sichuan Province conducting radiation diagnosis and treatment, including 280 tertiary institutions (8.18%), 647 secondary institutions (18.90%), 1 104 primary institutions (32.24%), and 1 393 unclassified institutions (40.68%). A total of 31 763 radiation workers were employed. The province had 9 056 radiation diagnosis and treatment devices, with 3 346 in tertiary institutions (36.95%), 2 057 in secondary institutions (22.71%), 1 510 in primary institutions (16.67%), and 2 143 in unclassified institutions (23.67%). The pass rate for initial inspections of sentinel hospital equipment was 96.00%, while the re-inspection pass rate was 97.41%. Devices over 10 years old accounted for 24.70%, those between 5 to 10 years for 62.10%, and those under 5 years for 13.20%. The annual total of radiation diagnosis and treatment visits reached 49.8 897 million. The province had 48 877 pieces of personal protective equipment for radiation, with an average of more than 13 protective items per device in nuclear medicine and interventional radiology. Compared to 2014, the number of institutions conducting radiation diagnosis and treatment increased by 27%, the number of radiation workers grew by 251%, and the frequency of radiation diagnosis and treatment activities rose by 215%.

The update of radiation equipment is slow, with most devices in use for 5 to 10 years, indicating a need for improved maintenance and monitoring awareness among medical institutions. All levels of medical institutions meet the requirements for the quantity of radiation protection supplies, and there is sufficient provision of protective items in interventional radiology and nuclear medicine projects. Given the increasing frequency of radiation diagnosis and treatment, it is recommended that relevant departments strengthen monitoring of radiation equipment protection to enhance the level of radiation diagnosis and treatment in medical institutions and ensure safe practices in these activities.

To analyze the physical exercise habits of urban and rural residents in Henan Province and their association with depression, anxiety disorders, and insomnia.

A stratified multi-stage random sampling method was employed to survey residents aged 18 and older in urban and rural areas of Henan Province. Data on demographic characteristics, physical exercise, and mental health status were collected using questionnaires and screening scales. Subsequent diagnosis of mental disorders was performed on individuals screened out by the scales, and analyses were conducted using χ² tests and logistic regression.

(1) Among 10 057 urban and rural residents, the proportions of individuals engaging in minimal, low, moderate, and high levels of exercise were 46.8%, 21.1%, 26.1%, and 6.0%, respectively. Statistically significant differences in exercise levels were observed across various demographics, including region, gender, age, education level, occupation, marital status, monthly income, smoking habits, drinking habits, health status, and mental health literacy (P<0.05). (2) Multivariate logistic regression analysis indicated that, after adjusting for demographic characteristics, lifestyle habits, and health status, participants engaging in moderate exercise had a lower risk of depression (OR=0.670, 95%CI: 0.470-0.954) and insomnia (OR=0.758, 95%CI: 0.651-0.883) compared to those who did not exercise. (3) Logistic regression results among different demographic groups showed that participants engaging in moderate exercise had a lower risk of insomnia in urban (OR=0.674,95%CI: 0.446-0.987), rural (OR=0.664, 95%CI: 0.547-0.805), male (OR=0.744, 95%CI: 0.592-0.935), and 18-35 age groups (OR=0.514, 95%CI: 0.313-0.842). In the female population, those engaging in moderate exercise had a lower risk of depression (OR=0.296, 95%CI: 0.171-0.512) and anxiety disorders (OR=0.613, 95%CI: 0.432-0.870) compared to non-exercisers. Additionally, compared to non-exercisers, participants with small (OR=0.774, 95%CI: 0.627-0.955), moderate (OR=0.682, 95%CI:0.56-0.827), and high levels of exercise (OR=0.542, 95%CI: 0.367-0.800) had lower risks of insomnia. Among the 36-59 and ≥60 age groups, participants engaging in moderate exercise had a lower risk of depression (OR=0.621, 95%CI: 0.374-0.932; OR=0.502, 95%CI: 0.284-0.887), anxiety disorders (OR=0.665, 95%CI: 0.460-0.960; OR=0.737, 95%CI: 0.582-0.925), and insomnia (OR=0.674, 95%CI: 0.545-0.833; OR=0.625, 95%CI: 0.482-0.812).

There are significant differences in physical exercise levels among different demographic groups. Participants engaging in moderate exercise have a lower risk of depression and insomnia, with a more pronounced correlation between physical exercise and mental disorders observed in females and older adults.

To explore the causal relationship between serum micronutrients and hypertension using Mendelian randomization, thereby promoting the prevention and health management of hypertension.

Fourteen serum micronutrients were selected as exposure factors, with hypertension as the outcome. Two-sample Mendelian randomization analyses were conducted based on genome-wide association study data from European populations, followed by multivariable Mendelian randomization analyses to identify variables that may have a causal relationship with the outcome, determining the independent effects of each exposure factor on the outcome.

Univariate Mendelian randomization analysis indicated a positive causal effect of elevated copper levels on the incidence of hypertension (P<0.05). Multivariable Mendelian randomization showed that the causal relationship between copper and hypertension remained significant after controlling for potassium and vitamin B6 (OR=1.028, 95%CI: 1.009-1.047, P<0.05).

There is a causal relationship between elevated copper levels and hypertension. However, the findings of this study require validation through randomized controlled trials.