To develop a sarcopenia cognitive behavior questionnaire for elderly in community and to test the reliability and validity of the questionnaire.

Based on cognitive behavior theory, the initial questionnaire was formed through literature review and expert consultation. We used convenience sampling method selected a total of 240 elderly people from 8 communities, including Fuhua Street community, Nanyang Road community, Mianfang Road community and Zijingshan Road community as survey objects from October to December 2022. Then, we modified the questionnaire and tested for reliability and validity.

The sarcopenia cognitive behavior questionnaire of community elderly included 3 dimensions and 23 items. The questionnaire’s total Cronbach’s coefficient was 0.903, and the split-half reliability was 0.872, and the test-retest reliability coefficient was 0.901 two weeks later. I-CVI of questionnaire was 0.857-1.000 and S-CVI/Ave was 0.988. Six common factors were extracted from exploratory factor analysis, and the cumulative variance contribution rate was 69.745%.

The sarcopenia cognitive behavior questionnaire of community elderly is scientific and practical, and can be used as a tool for clinical workers to evaluate the sarcopenia cognitive status of community elderly and their prevention and management behavior.

To explore the association between the dietary diversity score (DDS) and the prevalence of possible sarcopenia among the elderly.

We conducted this analysis utilizing the cross-sectional data from the Chinese longitudinal healthy longevity survey and happy family study(CLHLS-HF, wave 2018 ). A standard developed by the Asian Working Group for Sarcopenia 2019(AWGS2019) was used to assess the possibility of sarcopenia. We have constructed four new indicators of DDS as follows: total diet, animal-based diet, plant-based diet, and plant-based diet without the consumption of legume products and nuts. We used the Logistic regression analysis to evaluate the association between the DDS of the total diet, animal-based diet, plant-based diet, and plant-based diet without the intake of legume products and nuts and possible sarcopenia. These associations were statistically adjusted for a variety of potential confounders. Sensitivity analysis and subgroup analyses were performed.

The analysis included 2 555 participants (mean age of 84.4 years). 1 244 (48.7％) were male and 1 311 (51.3％) were female. 1 632 elderly people had possible sarcopenia. DDS in the possible sarcopenia group was significantly lower than that in the non-sarcopenia group (P < 0.001). In our study, we found that participants with a higher DDS of the total diet (OR=0.544, 95% CI：0.405-0.732), animal-based diet (OR=0.613, 95% CI：0.423-0.888), and plant-based diet (OR=0.469, 95% CI：0.321-0.684) and plant-based diet without the consumption of legume products and nuts (OR=0.382, 95% CI：0.206-0.709) were at a lower risk of developing sarcopenia. In sensitivity analyses, the associations remained unchanged. In subgroup analysis, total diets, animal-based diet, and plant-based diet were still associated with a reduced risk of possible sarcopenia in the male population, while plant-based diet and plant-based diet without the intake of legume products and nuts were associated with a reduced risk of possible sarcopenia in the female population.

Taking a diversified diet may reduce the risk of developing sarcopenia. According to the findings of this study, adopting a diversified diet might reduce the risk of sarcopenia for older adults.

To explore the relationship between retinol and TTR protein, and to provide ideas for preventing Hereditary vitreous amyloidosis (HVA).

Twenty Gly83Arg mutant mice were constructed and bred (reference construction ^[2]) as the mutant group. After the irregular turbidity in the vitreous of the mutant mice eyeball was observed under slit lamp, the lens camera was used for photography, and B-ultrasound examination was conducted, and white clump-like turbidity was found in the posterior ocular region. After the turbidity in the vitreous cavity of the mice was confirmed, the mice were killed by cervical vertebrae dislocation, and DNA in liver cells was extracted and sequenced to confirm the successful construction of mutant mice. At the same time, 20 wild type C57BL/6 mice were fed as the control group, 4 mice in the mutant group and 4 mice in the control group were randomly selected from the number table, and the retinol concentration was determined by high performance liquid chromatography combined with mass spectrometry. Three mice in the mutant group and three mice in the control group were selected from a random number table. After grinding the eyeball tissues, RNA and protein were extracted. TTR gene mRNA expression, SDS-PAGE protein gel color development was detected by fluorescent quantitative PCR, and TTR gene protein expression was detected by Western Blot. The difference at P < 0.05 was statistically significant.

The mutation site was consistent with the site successfully modeled by our research group in the previous stage. Heterozygous mutation occurred at the G base at the 107th position of exon 3 (C.107G →C), and the codon of the amino acid at the 83rd position was mutated from GGC to CGC (Gly83Arg). The concentration of retinol in eye of mutant mice was lower than that of normal mice in control group, the content of retinol in liver tissue of mutant mice was higher than that in control group, and the content of retinol in blood of mutant mice was lower than that in control group (eyeball: t=6.600, P=0.022; liver: t=9.045, P<0.001; blood: t=4.343, P=0.012). Fluorescence quantitative PCR results showed that mRNA expression in eyeball tissue of mutant group was lower than that of control group (t=5.764，P=0.004). SDS-PAGE protein gel color showed that protein bands appeared in the mutant group near 11kD. Western Blot showed that TTR gene protein expression in mutant group was lower than that in control group (t=37.48，P<0.001).

The deposition of vitreous amyloid protein in the eyeball tissue of Gly83Arg mutant mice may be related to the increase of mutant TTR protein and the decrease of retinol, which has important significance for future prevention and treatment.

To identify the types of social networks of the elderly in China, to analyze their population distribution characteristics, and to explore the association between different social network types and health behaviors, so as to provide reference for developing personalized health intervention strategies.

Using the data from the 2018 Chinese Longitudinal Healthy Longevity Survey, latent class analysis was used to identify the types of social networks in the elderly. The Multinomial Logistic Regression Analysis and Apriori algorithm were used to analyze the association between different social network types and health-related behaviors.

A total of 6 184 elderly people was included in this study. The social network types of the elderly in China were divided into family type (45.97%), couple type (41.91%) and widowed type (12.12%).Results from the Multinomial Logistic Regression Analysis showed that the elderly who were older (OR=1.141, 95% CI: 1.132-1.149), female (OR=4.472, 95% CI: 3.846-5.201), in ethnic minority (OR=1.454, 95% CI:1.037-2.040), and had sufficient sleep (OR=1.158,95% CI: 1.010-1.328) were more likely of the family-oriented social network. The elderly who were older (OR=1.120, 95% CI: 1.109-1.131), female (OR=4.913, 95% CI: 4.007-6.026) and had sufficient sleep (OR=1.247,95% CI:1.039-1.498) were more likely of the widowed-like social network.Strong association rules of the association rule is: drinking, smoking, and exercise of the elderly social network for couples.

The couple type is more likely to have unhealthy behaviors in the elderly in China, and its social network is smaller and its social support is single. Therefore, it is necessary to enrich the social network, enhance social support, and carry out intergenerational intervention to improve the healthy behavior of the elderly.

To explore the impact of health checkups on the utilization of inpatient services for patients with chronic diseases to promote the utilization of health checkups and alleviate the burden of hospitalization.

Utilizing data from four waves of the China Health and Retirement Longitudinal Study conducted in 2011, 2013, 2015, and 2018, this study employed coarsened exact matching (CEM) and the difference-in-difference（DID）and BK method to analyze the impact of health checkups on the utilization of inpatient services for patients with chronic diseases, along with examining its influencing mechanisms.

Health checkups were found to increase the probability of hospitalization by 34.4% (β=0.344, 95% CI: 0.132-0.556) and reduce hospitalization costs by 3 145 yuan (β=-3 145.470, 95% CI: -6 074.532--216.407) for patients with chronic diseases. Exercise was identified as mediating the association between health checkups and hospitalization (β=-0.184, 95% CI: -0.323--0.045). Age was observed to negatively moderate the relationship between health checkups and hospitalization (β=-0.531, 95% CI: -0.804--0.258). Patients with multiple chronic diseases and groups with health insurance were more significantly affected by health checkups.

Health checkups increase the likelihood of hospitalization and reduce hospitalization costs for individuals with chronic diseases. Promoting healthy lifestyles among those with chronic conditions is essential, with a specific focus on groups susceptible to health risk shocks.

To discuss the risk factors of imported food through the multi-dimensional comparative analysis of the food information published by the General Administration of Customs and to provide data reference for supervisors, and to improve the supervision of imported food.

This paper mainly collected and summarized the 48 periods of non-permitted entry food data released by the Import and Export Food Safety Bureau of the General Administration of Customs during 2019-2022 and made a comparative analysis of the sources of non-permitted entry food, food production enterprises, product types, non-permitted entry facts and other dimensions.

From 2019-2022, there were a total of 9 506 batches of food that were not allowed to enter the country, and the food that was not allowed to enter the country came from 112 countries and regions, with the highest proportion of food batches from Japan. The food came from 3 941 production enterprises, where11 countries or regions holding non-permitted entry food production enterprises, accounting for more than 50% of the total number of enterprises. According to the HS code, there were six categories and 21 chapters of non-permitted entry products, and the main non-permitted entry foods were HS03, 21, 19 and 22 products, more than 60% of the total unqualified. According to the analysis of the causes of nonconformity, there were 15 kinds, and the top 6 nonconformity causes accounted for 86% of the total nonconformity batches. There was a total of 268 types of unqualified items related to quality and safety, totaling 3 469 items.

The overall number of batches of unauthorized imported food during the period of 2019-2022 is a rising and then falling trend, and the unauthorized imported food is more concentrated in the countries of origin, manufacturers, categories of unauthorized food, reasons for failure, and quality-related unqualified items. The multi-dimensional comparative analysis provides data reference for regulators and consumers and helps the government to improve the regulation of imported food.

To analyze the unqualified situation of blood test of voluntary blood donors in Changsha from 2018 to 2022, and to provide reference for the formulation of recruitment strategy of voluntary blood donors.

From January 2018 to December 2022, 792 508 voluntary blood donors in Changsha area (Changsha Blood Center) were selected as the subjects of this study. Blood samples were tested by enzyme-linked immunosorbent assay (ELISA) twice and nucleic acid detection (NAT) once. The unqualified blood tests in different years and populations were described.

The total unqualified rate of voluntary blood donors in Changsha from 2018 to 2022 was 1.76%, with the highest rate in 2018 (1.90%) and the lowest rate in 2022 (1.56%). The total failure rate of alanine aminotransferase (ALT) and hepatitis B virus surface antigen (HBsAg) ranked first (1.00%) and second (0.62%), respectively. There were significant differences in the unqualified rate of blood test for HBsAg, anti-HCV, anti-HIV, anti-TP, ALT and the single reactivity rate of nucleic acid test (NAT) among different years (P<0.05), and there were significant differences in the unqualified rate of blood test for volunteer blood donors with different gender, age, education level and occupation (P<0.05).

The total unqualified rate of blood testing of voluntary blood donors in Changsha from 2018 to 2022 has significantly decreased compared with that before 2018 in the same region, and ALT and HBsAg are the main reasons for unqualified blood testing. In order to promote the sustainable and healthy development of voluntary blood donation in Changsha, it is necessary to further improve the blood testing technology, optimize the blood collection and supply process, and then improve the accuracy of the test results.

To assess the prevalence and identify risk factors of thyroid diseases among perimenopausal women, providing a theoretical basis for scientific prevention and control measures.

533 perimenopausal women in Shenzhen, aged 40-60, were statistically analyzed for demographics, living habits, residential pollution, and biochemical indicators, with multionmial logistic regression used to assess their links to thyroid disease occurrence.

The prevalence of thyroid dysfunction in perimenopausal women was 37.0% (197/533). The prevalence of thyroid nodules was 49.7% (265/533) and 14.3% (76/533) in other thyroid ultrasound abnormalities types. The prevalence of thyroid ultrasound abnormalities accordingly tended to increase with age. Multionmial logistic regression analysis showed that systolic blood pressure (OR=1.066, 95% CI:1.024-1.110) and history of thyroid disease (OR=6.853, 95% CI:1.537-30.555) were the risk factors of thyroid dysfunction; 55-60 years old (OR=5.011, 95% CI:1.938-12.956), occupation of clerical staff (OR=3.332, 95% CI:1.196-9.282), diastolic blood pressure (OR=1.050, 95% CI:1.004-1.098), and history of thyroid disease (OR=6.704, 95% CI:1.820-24.698) were the risk factors of thyroid nodules; TGAb antibody positivity (OR=3.763, 95% CI: 1.177-12.031), occupation of technician (OR=4.196, 95% CI:1.259-13.983), systolic blood pressure (OR=1.036, 95% CI:1.005-1.068) and history of thyroid disease (OR=17.917, 95% CI:5.107-62.851) were the risk factors of thyroid dysfunction and ultrasound.

Thyroid dysfunction and nodule rates among perimenopausal women in Shenzhen (37.0% and 49.7%) exceed China's averages (19.9% and 23.7%). Heightened screening and early action for thyroid issues in this group are needed.

Quality of life and survival time are important contents of palliative care services for patients with advanced cancer. Therefore, investigating the measurement method of quality-adjusted life years for patients with advanced cancer could provide a basis for the evaluation of palliative care services for this population.

The relative importance of each index in the Quality-of-Life Scale of Cancer Patients was compared by expert consultation method and hierarchical analysis was used to obtain the weight of each index.

In the Quality-of-Life Scale of Cancer Patients, the weights of primary indicators from high to low included physical symptoms, mental psychology, and social relations. The weights of physical symptoms from high to low included pain, side effects of treatment, sleep, daily life, appetite, fatigue, and facial expression. The weights of mental psychology from high to low included their cognition of disease, attitude, and spirit to treatment. The weights of social relations included the care and understanding of family and the understanding and cooperation of colleagues.

The weights of each quality of life scale indicator are obtained by hierarchical analysis. Then the different quality-of-life survival time is converted into quality-adjusted life years, which can provide a basis for the influence of palliative care services on the quality-adjusted life years of patients with advanced cancer.

To understand the current situation, influencing factors, and urban-rural heterogeneity of overweight/obesity among the elderly in China, so as to provide basis for formulating corresponding intervention measures.

The data of people age ≥ 60 years old in China Family Panel Studies (CFPS) database in 2020 were used. Unconditional binary logistic regression was used to analyze the influencing factors of overweight/obesity in the elderly.

A total of 4 870 eligible samples were included in this study, of which 1 903 were overweight/obese, and the overweight/obesity rate was 39.08%(33.10% in rural areas and 36.56% in urban areas). People aged 60-69 (OR = 2.145, 95%CI: 1.559-2.951), 70-79 years old (OR = 1.752, 95%CI: 1.270-2.416), suffering from chronic diseases (OR = 1.215, 95%CI: 1.061-1.392), smoking (OR = 0.651,95%CI: 0.556-0.763), living in the central China (OR = 1.255, 95%CI: 1.052-1.498), eastern China (OR = 1.378, 95%CI:1.169-1.623), living in town (OR = 1.366, 95%CI: 1.193-1.565), with primary school education (OR = 1.214, 95%CI: 1.034-1.427), retirement pension (OR = 1.342, 95%CI: 1.126-1.599), and endowment insurance (OR = 0.841, 95%CI: 0.739-0.956) were the influencing factors of overweight/obesity in the elderly. People aged 60- 69 (OR = 2.333, 95%CI: 1.561-3.487), 70-79 years old (OR = 2.057, 95%CI: 1.372-3.084), suffering from chronic diseases (OR = 1.364, 95%CI: 1.132-1.645), smoking (OR = 0.705, 95%CI: 0.563-0.881), living in the eastern region (OR = 1.278, 95%CI: 1.006-1.623). with primary school education (OR = 1.397, 95%CI: 1.104-1.768), and retirement pension (OR = 1.284, 95%CI: 1.046-1.577) were the influencing factors of overweight/obesity of the urban elderly. People aged 60-69 years old (OR = 1.779, 95%CI: 1.048-3.021), smoking (OR =0.594, 95%CI: 0.473-0.747), living in central China (OR = 1.28, 95%CI: 1.010-1.647), eastern China (OR = 1.481, 95%CI:1.176-1.867), retirement pension (OR = 1.424, 95%CI: 1.003-2.022), and new rural cooperative medical system (NCMS) (OR =0.475, 95%CI: 0.261-0.862) and health insurance for urban/rural residents (OR = 0.615, 95%CI: 0.380-0.995) were the influencing factors of overweight/obesity of the rural elderly.

The influencing factors of overweight/obesity in the elderly are multi-level and multi-dimensional and vary between urban and rural areas. Timely and targeted preventive strategies and preventive measures should be taken from individual to macro dimensions, based on the current situation of urban-rural differences in China.