ABSTRACT: Cervical cancer is the most common malignant tumor of female reproductive system. There are many pathologic diagnosis methods for cervical cancer or suspected patients in early clinical stage..In recent years, with the vigorous development of the field of examination, the emergence of immunohistochemical pathological diagnosis technology, molecular pathological diagnosis technology, and RNA in situ hybridization (RNA Scope) technology has brought new directions for the clinicopathological diagnosis of cervical cancer. By summarizing the epidemiology of cervical cancer, this study showed the current situation of the disease, analyzed the clinical application of various pathological diagnostic techniques, and discussed the advantages and disadvantages of different pathological diagnostic techniques. At the same time, the future development trend of clinicopathological diagnosis of cervical cancer was explored from a macroscopic point of view. This provides important data support for the establishment of cervical cancer risk prediction model and pathological diagnosis system framework, and adopts the best pathological diagnosis technology to analyze the potential risk or pathological classification of patients. Finally, it can meet the personalized diagnosis and treatment needs of patient groups and highlight its high-quality medical service level.

Objective In order to clarify the pollution situation of heavy metal elements in water basins and improve the detection accuracy of heavy metal elements, a study on the detection of heavy metal elements in water basins based on inductively coupled plasma mass spectrometry (ICP-MS) method is conducted. Methods By selecting appropriate detection chemical reagents, ensure the accuracy and sensitivity of the detection process. The preparation of chromatographic columns suitable for ICP-MS method provides strong support for the determination of heavy metal elements. To ensure the reliability of the test results, the collection and pretreatment process of water basin samples has been strictly standardized. In the process of chromatographic column screening and heavy metal element determination, the optimal chromatographic column is selected for quantitative determination of heavy metal elements. Results The detection method based on ICP-MS has high detection accuracy for heavy metal elements in water basins, with small errors compared to actual situations. Conclusion This provides strong technical support for monitoring heavy metal pollution in water basins.

ABSTRACT: Blood test is an important basis for the health assessment of animals, and plays an important role in the field of medical animals. In-depth analysis of the preservation conditions of blood specimens can greatly improve the accuracy of zoological testing, and accurate blood testing results can become an important reference for disease diagnosis and treatment. Scientific and reasonable preservation conditions can ensure the stability of the blood, and the health state of the animal body can also be reflected by the blood. This paper makes an in-depth analysis on the influence of the blood specimen preservation conditions on the animal blood test results, and puts forward several feasible specific strategies, in order to provide reference and help, provide an important guarantee for the treatment of animal diseases, and comprehensively promote the modern development of animal medicine in China.

This paper mainly studies the application of railway food testing and HACCP system, aiming to provide reference for the industry insiders, to ensure the safety and health of domestic railway food. By explaining the key points of railway food detection and their value, it analyzes the key points of railway food detection based on HACCP system, and conducts food detection combined with a train catering base. For fast food lunch boxes, food and drinking utensils, and production environment were tested, HACCP correction analysis was adopted based on the testing results, and corresponding remediation measures were formulated to improve the quality of food testing. The results show that the qualified rate of each index after rectification is between 80%~85%, which can guarantee the safety of food. It is found that HACCP system has obvious application value for railway food detection and can be further promoted.

Objective To establish an ultra high performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-MS/MS) method for simultaneous determination of the characteristic components of Ophiopogon japonicus and Liriope spicata (Thunb.) Lour. var. prolifera Y. T. Ma saponins B and Liriope muscari (Decne.) Baily saponins C in Xuanmai Ganju granules. Methods Xuanmai Ganju Granules were extracted by methanol ultrasound and analyzed using OMNI Orca${\mathrm{C}}_{18}$chromatography column (${2.1}\mathrm{\;{mm}}\times {100}\mathrm{\;{mm}},{2.6\mu }\mathrm{m}$). The mobile phase was eluted using a gradient elution of${10}\mathrm{{mmol}}/\mathrm{L}$ammonium acetate solution and methanol. The column temperature was set to${40}^{\circ}\mathrm{C}$, and the injection amount was${1\mu }\mathrm{L}$. The contents of methyl ophiopogon flavanone$\mathrm{A}$and methyl ophiopogon flavanone$\mathrm{B}$in Ophiopogon japonicus, as well as saponin B in Liriope spicata (Thunb.) Lour. var. prolifera Y. T. Ma and saponin C in Liriope muscari (Decne.) Baily, using an electrospray ion source (ESI), a simultaneous scanning mode of positive and negative ions, and a multi reaction monitoring mode. Results Methyl ophiopogon flavanone A, methyl ophiopogon flavanone B, saponin B from Liriope spicata (Thunb.) Lour. var. prolifera Y. T. Ma, and saponin C from Liriope muscari (Decne.) Baily have good linear relationships within their respective ranges, with $r$ values greater than 0.996 and recovery rates ranging from 90.6% to 99.8%. Conclusion This method has the advantages of simple and fast pre-treatment, high sensitivity, and accurate and reliable results. It can be used to determine the feeding situation of Ophiopogon japonicus mixed with Liriope spicata (Thunb.) Lour. in commercially available Xuanmai Ganju Granules and provide legal basis for regulatory authorities.

Objective To establish a rapid method for the determination of benzo [a] pyrene in vegetable oil by liquid-liquid extraction-high performance liquid chromatography. Methods The sample was extracted by acetonitrile oscillation, and after centrifugation, the supernatant was taken and passed through${0.22\mu }\mathrm{m}$filter membrane, and injected into high-performance liquid chromatograph for measurement. Results were quantified using matrix external standard method. Results Benzo [a] pyrene had a good correlation in the linear range of${0.5}- {20.0}\mathrm{{\mu g}}/\mathrm{{kg}}$, and the correlation coefficient${r}^{2}$was$\geq {0.9990}$. The recoveries at 0.5,2.0 and${10.0}\mathrm{{\mu g}}/\mathrm{{kg}}$were${87.0}\%\sim {111.2}\%$, and the relative standard deviations were${2.2}\%\sim {14.2}\%$. The limits of detection and quantitation were${0.2\mu }\mathrm{g}/\mathrm{{kg}}$and${0.5\mu }\mathrm{g}/\mathrm{{kg}}$, respectively. Conclusion The method is simple, rapid and accurate, and is suitable for the determination of benzo [a] pyrene in vegetable oil.

The normal module operating temperature (NMOT) of photovoltaic modules plays an important role in analyzing the actual power generation performance of the modules, but it is difficult to accurately measure. In the experimental teaching of heat transfer course, the introduction of photovoltaic module temperature rise cases has expanded the scope of teaching cases, cultivated students' ability to solve engineering problems of photovoltaic module temperature rise modeling using heat transfer mechanisms, and broadened their knowledge of factors affecting photovoltaic power generation. Finally, the theoretical calculation method for the temperature rise characteristic model of photovoltaic modules was analyzed, and experiments were designed to verify the accuracy of the model. The validated model was used to calculate the NMOT of photovoltaic modules

This study aims to develop a rapid detection method based on multiplex real-time quantitative PCR technology for accurate identification and quantification of common pathogenic bacteria on face masks, to assess the hygienic condition of masks and control nosocomial infections. Methods Using TaqMan probe-based real-time fluorescence quantitative PCR as the core technology, specific primers and fluorescently labeled probes were designed and optimized for simultaneous quantification of specific gene sequences from three pathogens through one-tube multiplex detection. The experiment began by screening specific target genes and sequences from the NCBI database and designing primers and probes. Subsequently, quality testing of primers and probes was conducted, and standard samples were prepared through amplification of specific sequences and TA cloning. Finally, standard curves were established, and mask samples were tested. Results After optimizing experimental conditions, an efficient and stable multiplex real-time quantitative PCR detection system was successfully established. The detection efficiency of standard samples for three pathogens was evaluated at different concentration gradients. The detection data showed that the primers and probes had high specificity for target pathogens, and the amplification efficiency and signal intensity met experimental requirements. In the detection of mask samples, the method accurately quantified the genomic DNA copy numbers of Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus hemolyticus, thereby assessing the degree of microbial contamination on masks. Conclusion The rapid detection method for pathogenic bacteria on masks based on multiplex real-time quantitative PCR technology demonstrates high sensitivity, specificity, and speed, providing strong technical support for clinical and public health

Objective To study the determination of mercury and arsenic content in soil by microwave digestion/atomic fluorescence method. Methods In this study, microwave digestion was used as the pretreatment, by optimizing the digestion temperature, digestion solution dosage and other parameters, digested with aqua regia (a mixture of hydrochloric acid and nitric acid), and the contents of mercury and arsenic in the samples were determined by atomic fluorescence. Results The results showed that the correlation coefficients of the standard curves of mercury and arsenic were 0.9996 and 0.9999, and the detection limits of the method were 0.0011 mg/kg and${0.0096}\mathrm{{mg}}/\mathrm{{kg}}$, respectively. The relative standard deviation of parallel experiments was${1.3}\%\sim {8.7}\%$, all of which were within 10%. The recoveries were 91.2%~117.4% and 86.0%~98.0%, respectively. The measured values of the certified reference materials are all within the theoretical range. Conclusion By optimizing digestion temperature, digestion solution dosage and other parameters, the method is accurate, simple, reliable and has good practicability.

Objective To prepare cultivated soil standard material for agricultural soil testing process to ensure that the test process is closer to the actual test samples. Methods The candidate samples prepared by drying, crushing, grinding, mixing, packing, using the$F$test uniformity test, examined the long-term stability of the candidate in storage for more than 18 months and short-term stability of simulated transportation conditions, invited 10 long undertake the soil standard material value laboratory cooperation value, using a variety of test methods and the current advanced and reliable test methods, copper and nickel test, the sample with the average as the specified value, the final synthesis uncertainty. Results Developed by soil composition analysis standard material uniformity is good, for 18 months of stability monitoring, through the calculation evaluation for 5 years, the fixed value project comprehensive accurate, reasonable uncertainty assessment, the relative extension of copper uncertainty is${6.5}\%$, the relative uncertainty of total nickel is${6.7}\%$, better than similar concentration of other same grade of soil national standard material. Conclusion The standard material is widely used and can be used for soil composition analysis in agriculture, forestry, environment and geochemistry.