Information management is an inevitable trend in the management of monitoring laboratories, more and more environmental monitoring institutions are starting to build LIMS, promote the development of environmental monitoring towards automation, standardization, and effectiveness. Analyses the current status of LIMS construction in various industries in China, taking Chongqing ecological environment monitoring business management system as an example, propose the design of system functional, summarize the experience of successful system implementation, provide references measures for vertical management of environmental monitoring institutions.

Spinning disk confocal microscope isa widely used optical instrument. This article take the live cell spinning disk confocal system of the large-scale instrument sharing platform of Wenzhou Medical University as an example, to introduce the basic technical principles and open sharing status of this instrument. Based on actual operating conditions, thisarticle explores the content and methods of training to further improve the effectiveness and feasibility of the training mode, providing a reference for relevant technical personnel.

This paper discusses the importance and practical path of cultivating innovative talents by using the open sharing platform of large instruments and equipment in testing laboratory. In view of the challenges such as resource limitation, lack of professional training and defect of scientific research environment in the cultivation of innovative talents for large instruments and equipment, the paper puts forward some solutions such as building operation and maintenance team, developing innovative training projects and establishing skill training system. It is emphasized that these measures can stimulate students' innovative thinking and practical ability, and realize the cultivation of innovative talents based on the open sharing platform of large-scale instruments and equipment for testing.

In the context of increasingly serious global diseases and health problems, the importance of biosafety concept has become increasingly prominent. As a country with biological diversity, China faces more serious public health risks. Laboratory safety, as an important link to strengthen biosafety protection, especially for the safety of pathogenic microorganism laboratories, is an important link to reduce the exposure and spread of dangerous goods, and ensure public health and life safety. Scientific laboratory safety management is of great importance. In order to improve the management level of pathogenic microbiology laboratory, the application of digital technology and the construction of information laboratory record management platform are of great application significance, which can effectively realize an efficient, integrated and standardized laboratory management mechanism, and provide support for the optimization and construction of public facilities such as pathogenic microbiology laboratory. This study will mainly explore the optimization path of the construction of the pathogenic microbiology laboratory record management platform, combined with the development requirements of the industry, social needs, etc., to build an efficient management system and improve the quality of laboratory safety management.

Objective To observe and analyze the clinical effect of drychemistry and urine sediment. Methods This study selected 150 patientsin our hospital from January 2022 to June 2022, divided into threegroups, experimental group of 50 patients with routine urine test usingdry chemical method, observation group of 50 patients with urine routinetest, the control group of dry chemistry method and urine sediment, andthe detection of the index of patients in urine routine test. Results The comparison of each index in the experimental group and theobservation group with routine urine test was less than that of urineroutine test in the control group using the combined method of drychemistry and urine sediment; the misdiagnosis rate in the experimentalgroup with a routine urine test using dry chemistry was $8\%$ , the missed diagnosis rate was 14%,the total error rate was 22%; the misdiagnosis rate in the observationgroup using the routine urine test was 10%, the missed diagnosis ratewas ${18}\%$ , the total error ratewas ${28}\%$ ; the misdiagnosis rateof the control group with routine urine test using combined drychemistry and urine sediment was 2%, the missed diagnosis rate was 2%,the total error rate was 4%, the total error rate of the experimentalgroups with routine dry chemistry urine testing and the observationgroups with routine urine sediment testing was greater than that of thecontrol group with routine urine testing using combined dry chemistryand urine sediment, the statistical significant difference wassignificant $\left({P<{0.05}}\right)$ . Conclusion After the active cooperation ofrelevant researchers and patients, the clinical effect observation ofurine routine test shows that the combination of dry chemistry and urinesediment is the best clinical effect.

Objective To explore and analyze the value of seruminflammatory indexes, D-dimer (D-D) and bone biochemical indexes inwound healing of patients with fractures. Methods The clinical data of92 patients with fracturesin the hospitalwere retrospectively analyzedfrom March 2021 to February 2023. The above patients were divided intodelayed group $(n ={47}$ , delayedfracture healing) and normal group $(n={47}$ , normal fracture healing). The levels of high-sensitivityC-reactive protein (hs-CRP), procalcitonin (PCT), D-D and bonebiochemical indexes (bone gla protein (BGP), type I procollagen aminoterminal peptide (PINP), type I collagen cross-linked carboxy terminalpeptide (CTX), bone alkaline phosphatase (BALP) were compared betweengroups. Pearson analysis was used to analyze the correlation between theabove indexes and delayed fracture healing. Receiver operatingcharacteristic curve (ROC) was adopted to evaluate the predictive valueof the above indexeson predicting delayed fracture healing. Results Thelevels of hs-CRP, PCT, D-D and CTX in delayed group were higher thanthose in normal group $\left({P<{0.05}}\right)$ while the levels of BGP, PINP and BALP werelower than those in normal group $\left({P<{0.05}}\right)$ . Serum hs-CRP, PCT, D-D and CTX in patientswith fractures were positively correlated with delayed fracture healing $\left({P <{0.05}}\right)$ , andBGP, PINP and BALP were negatively correlated with delayed fracturehealing $\left({P<{0.05}}\right)$ . Under the ROC curve, the areas under thecurves (AUCs) of hs-CRP, PCT, D-D, CTX, BGP, PINP, and BALP inpredicting delayed fracture healing were 0.693, 0.810, 0.722, 0.763,0.746, 0.868 and 0.839, the sensitivities were 59.57%, ${59.57}\%,{72.34}\%,{59.57}\%,{85.11}\%,{93.62}\%$ and 76.60%, and the specificities were 78.72%,97.87%,65.96%,89.36%,59.57%, 72.34% and 80.85% respectively. Conclusion hs-CRP, PCT, D-D andbone biochemical indexes are closely related to delayed fracture healingin patients with fractures, which should be paid attention to inclinical practice.

Objective This study aims to compare and evaluate theperformance and applicability of two ammonia $\left({\mathrm{{NH}}}_{3}\right)$ detection methods in the field and laboratory. Methods The C16 portable ${\mathrm{{NH}}}_{3}$ gas detectorand GBZ/T 160.29-2004 ammonia Nessler’s reagent spectrophotometricmethod were used to perform 10 repeated measurements of 5 differentconcentrations $\left({{9.3}\sim {148.8}\mathrm{{mg}}/{\mathrm{m}}^{3}}\right)$ of ${\mathrm{{NH}}}_{3}$ standardgases. Results The results showed that the C16 portable detectorexhibited a highly linear relationship $\left({r ={0.9998}}\right)$ in fieldapplications, which was highly correlated with the concentration ofstandard gases; The GBZ/T 160.29-2004 method provides higher precisionmeasurement values in laboratory analysis. The relative standarddeviation and relative error of both methods are less than ±5%,demonstrating good precision and accuracy. The research conclusionindicates that the C16 portable detector is suitable for rapid on-sitedetection and emergency response, while the GBZ/T 160.29-2004 method ismore suitable for laboratory analysis that requires high precision. Conclusion This study provides a scientific basis for selectingappropriate ${\mathrm{{NH}}}_{3}$ detection methods, which is of great significance for improving thequality of environmental monitoring and occupational healthassessment.

Objective To develop a scientific method for identifyingsea sand to differentiate it from river sand. Methods Qualitative andquantitative analysis of sea sand is conducted through its sensorycharacteristics, microscopic features, chemical and physical properties,and volumetric measurement methods. Results The chemical analysis showsthat sea sand is different from river sand with high chloride content(≥0.01%) and high shell content (≥0.1%). The electrical conductivity ofsea sand is significantly higher than that of river sand. In terms ofquantitative analysis, the relative deviation of the sea sand weightcalculation method proposed in this study is ${0.25}\%\sim {0.87}\%$ , which is close tothe allowable error of $\pm {0.3}\%$ of the truck scale. Conclusion The identification method proposed inthis study can quickly and effectively distinguish sea sand, which is ofgreat significance in ensuring the safety of building materials.

Objective To understand the pathogenic spectrum compositionof bacterial diarrhea in Jiangxia District of Wuhan, to explore thevariation and epidemic changes of the main pathogenic bacteria inJiangxia District of Wuhan in recent years, and to provide scientificbasis for the diagnosis and prevention and control of bacterialdiarrhea. Methods 341 specimens of patients with bacterial diarrheasyndrome in Jiangxia District of Wuhan from 2021 to 2023 were collected,and a variety of intestinal pathogenic bacteria were detected, and thedrug resistance of the detected pathogenic bacteria was analyzed. Results The highest detection rate in 2021-2023 was Salmonella,accounting for 42.82%(146/341) of cases detected; next is Vibrio, with adetection rate of ${16.42}\%\left({{56}/{341}}\right)$ ; thethird place is Escherichia coli, with a detection rate of 6.74%(23/341).In the typing results, the most commonly detected strains of Salmonella,Vibrio, and Escherichia coli were Salmonella enterica, Vibrioparahaemolyticus, and pathogenic Escherichia coli, with detection ratesof 14.37%, 13.20%, and 3.52%, respectively. Among the 341 detectedbacteria, the age group of 20~40 had the highest number of detectedbacteria, with 223, accounting for 65.40% of the total; 118 strains weredetected in the age group of 40~60, accounting for 34.60% of the total.There is a statistically significant difference between the two agegroups $\left({{\chi }^{2}= {57.484}, P<{0.001}}\right)$ . Conclusion Salmonella is the main pathogenic bacteria of bacterial diarrhea in Jiangxia District of Wuhan from 2021 to2023.

Objective This study aims to conduct a compatibility testbetween human fibrinogen and inner packaging materials throughaccelerated testing, explore the stability of drugs, determine whetherinner packaging materials have an impact on product quality, and providenecessary information for formulation design, packaging, transportation,and storage. Methods Conduct accelerated testing using 3 batches of testsamples, and place them upright and inverted at a temperature of $\left({{25}\pm 2}\right){}^{\circ }\mathrm{C}$ for6months. During the experimental period, samples were taken at theend of the third and sixth months respectively, and tested according tothe accelerated stability key inspection items (appearance, vacuumdegree, reconstitution time, visible foreign matter, osmotic pressuremolar concentration, stability test, moisture, $\mathrm{{pH}}$ value, purity, total amountof human fibrinogen, coagulation activity, arginine hydrochloride,citrate ions, sterility test), and compared with the results of Month 0. Results After being stored at ${\left({25}\pm 2\right)}^{\circ }\mathrm{C}$ for6months, the relevant migration products met the requirements, andthere were no significant changes in the activity indicators. Otherbiochemical detection indicators met the requirements of the ChinesePharmacopoeia (2020 edition, Part III), and there were no significantdifferences in the changes of the detection results of each indicator. Conclusion The selected packaging system has no adverse effects on thequality of human fibrinogen, and the drug quality in the selectedpackaging container is stable and controllable, which can maintain itssafety and effectiveness in use. The inner packaging material has nosignificant impact on the quality of the formulation and has goodcompatibility.