Objective To establish an analytical method for the rapid determination of mold and yeast in dried rice noodles based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Methods GB 4789.15-2016 "National Food Safety Standard Food Microbiological Inspection Mold and Yeast Enumeration" was used to detect and analyze the molds and yeasts of dried rice flour in the laboratory from 2021 to 2022, and 10 wild strains were obtained, and identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Results According to statistics, the dried rice noodles sampled by the laboratory from 2021 to 2022 are contaminated by fungi. of which the total detection rate of mold and yeast is about 45.45%. Through MALDI-TOF MS identification, among the 10 wild strains, mold accounts for 80% and yeast accounts for 20%. Conclusion MALDI-TOF MS, as a rapid, sensitive, accurate and high-throughput microbial identification technology, has realized the rapid and specific identification of mold yeast in dried rice noodles, and can provide scientific basis for the detection of mold yeast in dried rice noodles.

Objective Due to the poor application effect of current technology in groundwater pollution source water quality monitoring, the recovery rate of pollutants is low, and the RSD is relatively high, aiming at the defects of linear method, the application of ion chromatography technology in groundwater pollution source water quality monitoring is proposed. Methods An automatic sampling device is used to collect water samples upstream of groundwater pollution sources and filter them. By diluting the standard solution of each pollutant, preparing the standard working solution, centrifuging the water sample with the centrifuge, testing the water sample with the ion chromatograph, identifying the pollutants according to the standard working curve and generating the corresponding ion chromatogram, calculating the mass concentration according to the ion content of the pollutant, so as to complete the water quality monitoring of groundwater pollution sources based on the ion chromatographic technology. Results The recovery rate of pollutants is above 90% and the RSD value is lower than 0.1, the application effect of this technology is relatively good. Conclusion The technique has good adaptability and reliability, can be used for water quality monitoring.

Objective To evaluate the uncertainty evaluation for the determination of imidacloprid in Caidian lotus root by ultra performance liquid chromatography-tandem quadrupole mass spectrometry. Methods According to GB/T 20769 - 2008 Determination of 450 pesticides and related chemicals residues in fruits and vegetables-Liquid chromatography-tandem mass spectrometry, the content of imidacloprid in Caidian lotus root was determined and the mathematical model was built. According to the provisions of JJF 1135-2005 Evaluation of measurement uncertainty in chemical analysis and JJF 10591-2012 Technical specification for evaluation and representation of measurement uncertainty, the uncertainty was quantified by scientific analysis, and the extended uncertainty of imidacloprid content in Caidian lotus root　was finally calculated. Results When the residue imidacloprid in Caidian　lotus root was ${0.322}\mathrm{{mg}}/\mathrm{{kg}}$ , the　relative standard synthetic uncertainty was 0.08689 and the expanded　uncertainty was ${0.0560}\mathrm{{mg}}/\mathrm{{kg}}$ . The　determination results were　 $\left({{0.322}\pm {0.0560}}\right)\mathrm{{mg}}/\mathrm{{kg}},P ={95}\%, k = 2$ . Conclusion The main sources of uncertainty in　the determination of imidacloprid in Caidian lotus root by ultra　performance liquid chromatography-tandem quadrupole mass spectrometry　are the preparation of standard solution, sample pretreatment and　measurement process of the measuring instrument.

Objective To evaluate the uncertainty evaluation for the determination of imidacloprid in Caidian lotus root by ultra performance liquid chromatography-tandem quadrupole mass spectrometry. Methods According to GB/T 20769 - 2008 Determination of 450 pesticides and related chemicals residues in fruits and vegetables-Liquid chromatography-tandem mass spectrometry, the content of imidacloprid in Caidian lotus root was determined and the mathematical model was built. According to the provisions of JJF 1135-2005 Evaluation of measurement uncertainty in chemical analysis and JJF 10591-2012 Technical specification for evaluation and representation of measurement uncertainty, the uncertainty was quantified by scientific analysis, and the extended uncertainty of imidacloprid content in Caidian lotus root　was finally calculated. Results When the residue imidacloprid in Caidian　lotus root was ${0.322}\mathrm{{mg}}/\mathrm{{kg}}$ , the　relative standard synthetic uncertainty was 0.08689 and the expanded　uncertainty was ${0.0560}\mathrm{{mg}}/\mathrm{{kg}}$ . The　determination results were　 $\left({{0.322}\pm {0.0560}}\right)\mathrm{{mg}}/\mathrm{{kg}},P ={95}\%, k = 2$ . Conclusion The main sources of uncertainty in　the determination of imidacloprid in Caidian lotus root by ultra　performance liquid chromatography-tandem quadrupole mass spectrometry　are the preparation of standard solution, sample pretreatment and　measurement process of the measuring instrument.

Objective This study aims to accurately quantify two key components in Baijiu (Chinese liquor) production and quality control: acetic acid and hexanoic acid. Methods A direct injection-gas chromatography (GC) technique was used for the rapid and precise simultaneous determination of acetic acid and hexanoic acid in Baijiu. Results Using this method, the detection limit for acetic acid reached 0.012 g/L and for hexanoic acid, 0.0050 g/L. All correlation coefficients exceeded 0.999. The relative standard deviations (RSD) were 0.32% for acetic acid and 0.33% for hexanoic acid, with spiked recovery rates of 96.15% for acetic acid and 96.69% for hexanoic acid, respectively. Conclusion The new detection method proposed in this study not only overcomes the limitations of traditional detection methods and gas chromatography in many aspects but also demonstrates high efficiency, sensitivity, and excellent selectivity. This method has significant practical implications for improving the production process of Baijiu, ensuring the consistency of product flavor, and safety.

Objective To explore and analyze the blood physiological and biochemical indicators of SPF grade SD rats of different ages and genders. Method 80 SPF grade SD rats were studied and randomly divided into four experimental groups at 4, 12, 24, and 48 weeks of age. The male to female ratio in each group was 1:1. All SD rats were analyzed for their blood physiological indicators using the XFA6030 animal blood cell analyzer; apply the Beckman Coulter AU5800 fully automated biochemical analyzer to analyze its blood biochemical indicators. Result Compared with SD rats aged 12, 24, and 48 weeks, SD rats aged 4 weeks showed significant differences in WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT, PCT, BA #, and BA%(P<0.05), and all indicators reached their peak at 24 weeks. Among the gender differences between male and female, SD rats aged 4 weeks showed differences in BA%; 12. There were differences in WBC and RBC between 24 week old SD rats; 24 week old SD rats showed differences in BA%; 48 week old SD rats showed differences in MCH and PCT, and all differences had P values $<{0.05}$ ; Compared with 12,24, and 48 week old SD rats,4-week old SD rats showed significant differences in AST, CHOL, TP, GLU, CRE, ALT, ALP, BUN, and TG (P<0.05). Among the gender differences between male and female, 4-week old SD rats showed significant differences in CRE and ALP; There were differences in AST, CHOL, and BUN among 12 week old SD rats; There were differences in ALP between 12 week old and 24 week old SD rats, and all P values of the differences were<0.05 . Conclusion There are statistical differences in blood physiological and biochemical indicators among SD rats of the same gender but different age groups. Similarly, there are also differences in blood physiological and biochemical indicators among SD rats of the same age group but different genders.

Objective To understand the pollution levels of bromate, coliforms, pseudomonas aeruginosa, nitrite (calculated as ${\mathrm{{NO}}}_{2}$ )] of packaged drinking barreled water in Guangxi in 2022. Methods According to the type of water and test items, all pollutions were tested and judged by GB 8538-2016 “National standard for food safety Test method for drinking natural mineral water”, GB 4789.3-2016 “Food safety national standard food Microbiology test Coliform count”, GB 5009.33-2016 “National standard for food safety Determination of nitrite and nitrate,” GB 19298-2014 “Food safety national standard Packaging drinking water”, GB 2762-2017 “Food safety national standard food Pollutant limits”. Results 204 batches of packaged drinking barreled water were collected. Pseudomonas aeruginosa was deteted in 55 batches with a detection rate of 26.96%. Not a single batch exceeded permitted level of bromate, coliform or nitrite. Conclusion Most products are safe, but the pollution of Pseudomonas aeruginosa is still an outstanding problem. Enterprises should strengthen the cleaning and isinfection of pipes and barrels and standardize transportation storage to ensure product safety.

Objective To investigate the levels of veterinary drug residues in chicken and eggs in chicken production bases in Liangshan Prefecture, and evaluate the dietary exposure risk. Methods There were 353 samples of chicken and eggs collected from 17 cities in Liangshan in 2022, then the residues of quinolones and quinoline were detected and analyzed by liquid chromatography-mass spectrometry, and the exposure risk was assessed by point estimate method. Results A total of 221 pieces of chicken were sampled in 2022, among 8 types of veterinary drugs were tested with the detection rate of 25.79%, and the unqualified rate was 1.81%. Among 132 eggs, only enrofloxacin residue was detected, the detection rate was 1.52%, the unqualified rate was 1.52%. The point estimate method was used to assess the dietary risk of veterinary drug residues, and its hazard quotient was lower than 1, indicating that the veterinary drug residues have a lower risk to human health. Conclusion Veterinary drug residues can be detected in some samples of chicken and eggs in Liangshan in 2022, and the risk residues to human health is low, but the health risks of caused by some undetectable veterinary drugs cannot be ignored.

Objective To evaluate the uncertainty in determination of olive oil total migration by gas chromatography spectrometry, determine the factors affecting the accuracy of the results and provide a basis for accurate control of the olive oil total migration. Methods Gas chromatography spectrometry was used to determine the olive oil total migration, and a mathematical model was established. According to relevant rules and standards for uncertainty evaluation, the uncertainty was evaluated including repeatability, sample quality and adsorbed olive oil quality, instrument calibration, standard curve fitting, soaking area, etc. Results When The total migration of olive oil in the sample is ${4.0}\mathrm{{mg}}/{\mathrm{{dm}}}^{2}$, the expanded uncertainty was ${1.3}\mathrm{{mg}}/{\mathrm{{dm}}}^{2}\left({P={95}\%, k = 2}\right)$ . Conclusion The uncertainty of olive oil total migration mainly comes from repeatability, standard solution preparation and standard curve fitting.

Objective To study the content level of vanillin in rice and the changes of vanillin in paddy rice processing. Methods Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to determine the contents of vanillin, methyl vanillin and ethyl vanillin in 209 batches of rice from different origins; at the same time, 16 batches of rice were hulled, milled or finished, and the rice and its processed products (brown rice, standard rice or polished rice) were tested. Results Methyl vanillin and ethyl vanillin were not detected in all rice, paddy and their processed products; vanillin was all detected in 209 batches of rice, and the content values were close to the normal distribution, mainly concentrated in ${100}\sim {222\mu}\mathrm{g}/\mathrm{{kg}}$ ; vanillin was detected in rice and its processed products (brown rice, standard rice or polished rice), and the content decreases with the processing accuracy. Conclusion The possibility of naturally occurring vanillin in rice, and the source is probably rice hull; the detection of vanillin in rice is not always illegal addition, the content level of vanillin in rice and paddy rice should be continuously studied and scientifically analyzed to provide scientific evidence for the market supervision department formulating reasonably the risk reference value of rice.

Objective Isolate and identify microorganisms that cause rancidity in vacuum packed rice dumplings, thereby helping enterprises to improve product quality and control of food safety. Methods Bromocresol purple dextrose broth and cooked meat medium base were used to cultivate the acescent rice dumplings; the microorganisms were isolated and purified by scribing in Rose bengal agar, Nutrient agar, the isolated strains were identified by colony morphology observation, cell morphology observation, 16S rRNA sequence analysis, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The isolated strains were inoculated into commercial sterilized rice dumplings respectively to verify that two strains caused rancidity of rice dumplings. Results Two isolated strains, namely Lelliottia amnigena and Wickerhamomyces anomalus, caused rancidity of rice dumplings. Conclusion Lelliottia amnigena and Wickerhamomyces anomalus in vacuum-packed rice dumplings can cause rancidity.

Objective Isolate and identify microorganisms that cause rancidity in vacuum packed rice dumplings, thereby helping enterprises to improve product quality and control of food safety. Methods Bromocresol purple dextrose broth and cooked meat medium base were used to cultivate the acescent rice dumplings; the microorganisms were isolated and purified by scribing in Rose bengal agar, Nutrient agar, the isolated strains were identified by colony morphology observation, cell morphology observation, 16S rRNA sequence analysis, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The isolated strains were inoculated into commercial sterilized rice dumplings respectively to verify that two strains caused rancidity of rice dumplings. Results Two isolated strains, namely Lelliottia amnigena and Wickerhamomyces anomalus, caused rancidity of rice dumplings. Conclusion Lelliottia amnigena and Wickerhamomyces anomalus in vacuum-packed rice dumplings can cause rancidity.

Biopharmaceutical cleanroom air conditioning plays an important role in ensuring the cleanliness and stability of a clean environment. By investigating and analyzing the actual situation of clean air conditioning systems in the biopharmaceutical industry, scientific and effective methods for clean air conditioning testing and debugging are proposed, which can accurately evaluate the cleanliness and performance of clean air conditioning systems. Meanwhile, the practical application effect of this method indicates that it can improve the cleanliness and stability of clean environments.

Food re-inspection refers to food production and operation of food safety supervision and sampling inspection conclusions have objections and in accordance with the law to the market supervision and management department to submit an application for re-inspection, market supervision and management department commissioned the re-inspection of the re-inspection of the backup samples for review and inspection of the behaviour.Re-inspection is an important way for food producers and operators to safeguard their legitimate rights and interests, an important link for market supervision departments to ensure the high quality of food safety supervision work, and an important measure to ensure the relatively fair and stable operation of the market. Because the retest conclusion serves as the final conclusion Therefore, the professionalism of the re-inspection institution is particularly important in the re-inspection work. Based on the perspective of the re-inspection institution, this paper analyzes the risk points of the food re-inspection acceptance work (i. e., contract review), so that the re-inspection institution can avoid potential risks and lay a solid foundation for the smooth progress of the whole re-inspection work.

Food re-inspection refers to food production and operation of food safety supervision and sampling inspection conclusions have objections and in accordance with the law to the market supervision and management department to submit an application for re-inspection, market supervision and management department commissioned the re-inspection of the re-inspection of the backup samples for review and inspection of the behaviour.Re-inspection is an important way for food producers and operators to safeguard their legitimate rights and interests, an important link for market supervision departments to ensure the high quality of food safety supervision work, and an important measure to ensure the relatively fair and stable operation of the market. Because the retest conclusion serves as the final conclusion Therefore, the professionalism of the re-inspection institution is particularly important in the re-inspection work. Based on the perspective of the re-inspection institution, this paper analyzes the risk points of the food re-inspection acceptance work (i. e., contract review), so that the re-inspection institution can avoid potential risks and lay a solid foundation for the smooth progress of the whole re-inspection work.

With the outbreak of the novel coronavirus in 2020, the demand and production of medical masks have shown a spurt of growth worldwide. In order to ensure the quality and safety of masks, countries around the world generally formulate testing standards for medical masks. This paper mainly compares and analyzes the detection standards of medical masks at home and abroad, and puts forward suggestions for the quality assurance and standard formulation of medical masks in China, which is of great significance to the field of public health.

With the trend of globalization and population growth, the physical and chemical testing laboratories of disease control institutions play a crucial role in disease prevention and control. However, these laboratories face a series of safety and quality risks. The purpose of this study is to identify and control safety and quality risks in physical and chemical testing laboratories of disease control institutions. Through literature review and case studies, we analyzed the main risk sources in current laboratory management and proposed a series of measures to address these risks. This study has important guiding significance for improving the safety and quality of physical and chemical testing laboratories in disease control institutions.

Compared with most of the current published instruments and equipment management risk research literature, generally only pay attention to the risk identification of individual links, and there are problems such as incomplete risk identification and analysis, which will affect the effectiveness of instrument and equipment risk management. In order to guide the inspection body and laboratory to carry out the risk management of instruments and equipment more reasonably, risk identification is carried out from the aspects of the whole life cycle and safety of instruments and equipment, and the identified risks are analyzed and evaluated, and risk disposal suggestions are put forward according to the evaluation results. Encourage inspection body and laboratory to always meet the requirements of management system and compliance operation in terms of instrument and equipment risk management.

Compared with most of the current published instruments and equipment management risk research literature, generally only pay attention to the risk identification of individual links, and there are problems such as incomplete risk identification and analysis, which will affect the effectiveness of instrument and equipment risk management. In order to guide the inspection body and laboratory to carry out the risk management of instruments and equipment more reasonably, risk identification is carried out from the aspects of the whole life cycle and safety of instruments and equipment, and the identified risks are analyzed and evaluated, and risk disposal suggestions are put forward according to the evaluation results. Encourage inspection body and laboratory to always meet the requirements of management system and compliance operation in terms of instrument and equipment risk management.

As the most basic medical means, drugs can not only effectively treat and prevent diseases, but also directly affect the health of users. With the continuous improvement of the number and output of drugs, the quality and safety of drugs are the core tasks of pharmaceutical enterprises that can not be ignored. As an important means to detect the risk of problems, the effect of inspection and detection is affected by many factors, such as personnel, equipment, environment and so on. For this, major pharmaceutical manufacturing enterprises have the obligation to perform their duties and strengthen drug quality detection. In the context of the rapid development of science and technology, automation technology, information sharing platform, real-time monitoring system and so on have provided strong support for the development of drug inspection. Data integrity runs through the whole process of drug inspection. It is an internal demand and an inevitable trend to pay attention to the construction of drug research and development laboratory information management system (LIMS). Based on this, starting from the background of drug research and development stage, this paper explores the construction and design content of drug inspection LIMS, and puts forward corresponding construction strategies on how to improve the quality management level of drug research and development, in order to provide exchange and reference.

In this paper, the equipment management, personnel training and quality control of the laboratory are comprehensively assessed by adopting quality control methods such as checklist evaluation and traceability management. Taking a food testing laboratory as an example, it quantitatively analyzes its quality management and gives the evaluation and improvement suggestions on equipment management, personnel training and quality control of the laboratory. Meanwhile, in the process of establishing a traceable management system, the management of quality control should also be strengthened to improve the accuracy and reliability of testing. Through the improvement and refinement of the laboratory, it can not only enhance the overall quality level of the testing laboratory, but also contribute to the maintenance of social public safety and health.

In this paper, the equipment management, personnel training and quality control of the laboratory are comprehensively assessed by adopting quality control methods such as checklist evaluation and traceability management. Taking a food testing laboratory as an example, it quantitatively analyzes its quality management and gives the evaluation and improvement suggestions on equipment management, personnel training and quality control of the laboratory. Meanwhile, in the process of establishing a traceable management system, the management of quality control should also be strengthened to improve the accuracy and reliability of testing. Through the improvement and refinement of the laboratory, it can not only enhance the overall quality level of the testing laboratory, but also contribute to the maintenance of social public safety and health.

Objective Deeply dig into the rapid inspection data, build a quality evaluation model for food rapid inspection work, and achieve targeted management and smart supervision of rapid inspection work. Methods Based on massive rapid inspection data, the current status and supervision model of food rapid inspection in farmers' markets were analyzed, and a quality evaluation model for rapid food inspection was constructed. Results By carrying out research on the big data system for food rapid inspection, improving the scientificity and effectiveness of the evaluation of rapid inspection work. Conclusion This study provides a scientific theoretical basis for accurate supervision, intelligent supervision and targeted supervision at the grass-roots level.

In order to solve the problems of significant increase in workload, cumbersome management work, duplication of materials and low review efficiency caused by the increase of open subject application year-by-year, this article elaborates and analyzes the various needs in the open project management process, based on the requirements of the project-oriented management system, and the laboratory open subject management system was constructed based on the requirements of subject management system. The system is developed based on SpringBoot microservice architecture, B/S(Browser/Server) architecture, and Java language development. It can complete project demand reporting, requirements review, requirements selection, project application collection, project application review, project project initiation, mid-term inspection results management, and final inspection, and realize the information management of the whole life cycle of laboratory open projects.

Food inspection and testing institutions need to obtain corresponding qualification recognition in order to provide verifiable data results to society. Familiarity with the CMA qualification recognition and review process is a condition for the normal operation of food inspection and testing institutions. This article is based on the requirements outlined in RB/T214-2017, RB/T215-2017, and related norms and testing standards. Using the example of the on-site assessment of the CMA qualification extension for the food testing laboratory at the Zhenjiang Food and Drug Supervision Inspection Center, this article conducts an analysis of non-conformities and proposes targeted improvements. Additionally, it summarizes some experiences and reflections in areas such as personnel, instrumentation, system documentation, environmental facilities, and method validation. These insights are intended to serve as a reference for relevant personnel and laboratories in their pursuit of normal operations and conducting CMA qualification assessment reviews.

Objective To evaluate the risk of pesticide residues in cucurbit vegetables in Guizhou Qiandongnan Prefecture. Methods LC-MS and GC-MS was used for determination of residues of the level of 50 pesticide residues in cucurbit vegetable samples collected randomly from Guizhou Qiandongnan Prefecture. Results It showed that Pesticide residues were detected in 15 samples of 100 cucurbit vegetables, 8 pesticides were detected, 20 times in total, The average value of food safety index in cucurbit vegetables related to pesticide residues did not exceed the national maximum residue limit in Guizhou Qiandongnan Prefecture. Conclusion Our findings suggest that the potential hazard level of pesticide residues in cucurbit vegetables in Guizhou Qiandongnan Prefecture is acceotable.

Objective To evaluate the risk of pesticide residues in cucurbit vegetables in Guizhou Qiandongnan Prefecture. Methods LC-MS and GC-MS was used for determination of residues of the level of 50 pesticide residues in cucurbit vegetable samples collected randomly from Guizhou Qiandongnan Prefecture. Results It showed that Pesticide residues were detected in 15 samples of 100 cucurbit vegetables, 8 pesticides were detected, 20 times in total, The average value of food safety index in cucurbit vegetables related to pesticide residues did not exceed the national maximum residue limit in Guizhou Qiandongnan Prefecture. Conclusion Our findings suggest that the potential hazard level of pesticide residues in cucurbit vegetables in Guizhou Qiandongnan Prefecture is acceotable.

Objective In the present paper, the influences of matrix effect (ME) to the quantification of imidacloprid, thiamethoxam and clothianidin in ginger and banana were evaluated, and especially, the influencing factors and compensating means of matrix effect in LC-MS/MS analysis were proposed. Methods In this research, the matrix effects of three neonicotinoid insecticides in ginger and banana were evaluated by LC-MS/MS analysis after QuEChERS pretreatment. Both matrix-matched calibration curves and solvent calibration curves were established for the assessment of matrix effect. Results The linear regression coefficients of the matrix-matched calibration curves were all above 0.999 and the precision of the measured sample results was better than that of the solvent standard. The recoveries and relative standard deviations of all compounds at matrix-matched calibration curves were 86.7%~103.0% and 2.1%~8.3%, respectively. Conclusion Matrix effects can compromise the precision and accuracy of the analysis results. These results also indicated that matrix effects were mainly affected by matrix concentration, the types and samples of matrices and the overall chemical structure of the pesticide. The linear regression coefficients and precision as well as accuracy were more satisfactory for all the pesticides when matrix-matched standard was used, which reflected an excellent compensating means of matrix effect. This can improve the accuracy of quantitative results, which is feasible in routine testing, and is of great significance for ensuring the quality and safety of ginger and banana products.

Objective In the present paper, the influences of matrix effect (ME) to the quantification of imidacloprid, thiamethoxam and clothianidin in ginger and banana were evaluated, and especially, the influencing factors and compensating means of matrix effect in LC-MS/MS analysis were proposed. Methods In this research, the matrix effects of three neonicotinoid insecticides in ginger and banana were evaluated by LC-MS/MS analysis after QuEChERS pretreatment. Both matrix-matched calibration curves and solvent calibration curves were established for the assessment of matrix effect. Results The linear regression coefficients of the matrix-matched calibration curves were all above 0.999 and the precision of the measured sample results was better than that of the solvent standard. The recoveries and relative standard deviations of all compounds at matrix-matched calibration curves were 86.7%~103.0% and 2.1%~8.3%, respectively. Conclusion Matrix effects can compromise the precision and accuracy of the analysis results. These results also indicated that matrix effects were mainly affected by matrix concentration, the types and samples of matrices and the overall chemical structure of the pesticide. The linear regression coefficients and precision as well as accuracy were more satisfactory for all the pesticides when matrix-matched standard was used, which reflected an excellent compensating means of matrix effect. This can improve the accuracy of quantitative results, which is feasible in routine testing, and is of great significance for ensuring the quality and safety of ginger and banana products.

Objective To separate and identify the miscellaneous bacteria in sterilized lactic acid bacteria beverages provided by an enterprise. Methods The miscellaneous bacteria of lactic acid bacteria beverages were isolated and purified using a variety of medias. The isolated strains were identified at the genus and species levels by analysis of ${16}\mathrm{\;S}$ rRNA sequence and matrix-assisted laser desorption/ionization time of flight mass spectrometry. Results A total of 16 strains were isolated, Among them, 5 strains of Acetobacter, 2 strains of Gluconobacter japonicus, 1 strain of Gluconacetobacter liquefaciens, 1 strain of Kocuria palustris, 1 strain of Streptomyces hydrogenans, 1 strain of Staphylocaccus saprophyticus, 1 strain of Bacillus sp, 1 strain of Novosphingobium aromaticivorans, 1 strain of Brevundimonas vesicularis, 2 strains of Yeast, most of which (n=8, 50%)were identified as Acetobacteraceae. Conclusion Acetobacter, Bacillus, Staphylococcus, Brevundimonas vesicularis, mold, Yeast and other microorganisms were isolated from the bactericidal lactobacillus beverage, most of which belongs to Acetobacteraceae.

Objective Based on gas chromatography-mass spectrometry (GC-MS) technology and partial least squares analysis (PLS-DA) metabolomics methods, observe the changes in serum metabolites of mice after administration of traditional Chinese medicine Yuanbaocao, and provide a preliminary explanation for the analgesic mechanism. Methods Establish two analgesic models, the hot plate method and the acetic acid writhing method, and use GC/MS to detect and analyze etabolites in mouse serum. After collecting data, PLS-DA method is used to detect and classify endogenous small molecule metabolites in the samples. Results The hot plate analgesic experiment showed that there were extremely significant differences $\left({P<{0.01}}\right)$ in the licking time between the aspirin group, the high and medium dose groups of Yuanbaocao mice, and the model group at 30 and 60 minutes. As the administration time prolonged, Yuanbaocao showed different inhibitory effects on pain symptoms, which was inferred to be related to the high or low blood drug concentration. Compared with the blank group, the number of body twists in the Yuanbaocao group mice decreased significantly $\left({P <{0.01}}\right)$ , indicating that Yuanbaocao has a certain degree of analgesic effect. There are significant differences in the metabolic characteristics of mouse serum, and further screening of metabolic pathways that have a significant impact on data classification identified that taurine, glutathione, arginine and proline, ascorbic acid 5 metabolic pathways are related to pain metabolism. Conclusion The Yuanbaocao group can reverse abnormal changes to a certain extent and has analgesic effects.

Objective To evaluate the precision and accuracy level of four Dimai DM79x blood cell analyzers in the most suitable working state of our department's daily indoor quality control by comparing the indoor quality control data, so as to improve the accuracy and consistency of test results. Methods In February 2023 (28 days), the indoor quality control data of four laboratory instruments using original low, medium and high fixed value quality control products and daily testing of a random outpatient fresh patient sample were collected. The items included: White blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), platelets (PLT), mean red blood cell volume (MCV), mean red blood cell hemoglobin volume (MCH), mean red blood cell hemoglobin concentration (MCHC) calculated mean (X), standard deviation (S) and coefficient of variation (CV), Coefficient of variation index (CVI) and standard deviation index (SDI) were used as precision and accuracy evaluation indexes respectively. Result The statistical analysis showed that the blood cell analysis of the DM79x instrument included 8 parameters, including WBC, RBC, HGB, PLT, HCT, MCV, MCH and MCHC. The coefficient of variation index of CVI of each instrument was different, and the indoor quality control result of SDI was &lt;2.0. The measured values of CVI and SDI of fresh sample comparison data are all within the required range. Conclusion Four Dimai DM79x automatic blood cell analyzers in our department have good precision and high accuracy by comparing the indoor quality control data between laboratories. The performance of the instrument can meet the work needs of clinical laboratories. Moreover, the accuracy and consistency of the results in different instruments can be ensured by comparing the fresh samples of patients.

Objective To evaluate the precision and accuracy level of four Dimai DM79x blood cell analyzers in the most suitable working state of our department's daily indoor quality control by comparing the indoor quality control data, so as to improve the accuracy and consistency of test results. Methods In February 2023 (28 days), the indoor quality control data of four laboratory instruments using original low, medium and high fixed value quality control products and daily testing of a random outpatient fresh patient sample were collected. The items included: White blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), platelets (PLT), mean red blood cell volume (MCV), mean red blood cell hemoglobin volume (MCH), mean red blood cell hemoglobin concentration (MCHC) calculated mean (X), standard deviation (S) and coefficient of variation (CV), Coefficient of variation index (CVI) and standard deviation index (SDI) were used as precision and accuracy evaluation indexes respectively. Result The statistical analysis showed that the blood cell analysis of the DM79x instrument included 8 parameters, including WBC, RBC, HGB, PLT, HCT, MCV, MCH and MCHC. The coefficient of variation index of CVI of each instrument was different, and the indoor quality control result of SDI was &lt;2.0. The measured values of CVI and SDI of fresh sample comparison data are all within the required range. Conclusion Four Dimai DM79x automatic blood cell analyzers in our department have good precision and high accuracy by comparing the indoor quality control data between laboratories. The performance of the instrument can meet the work needs of clinical laboratories. Moreover, the accuracy and consistency of the results in different instruments can be ensured by comparing the fresh samples of patients.