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Objective Based on gas chromatography-mass spectrometry (GC-MS) technology and partial least squares analysis (PLS-DA) metabolomics methods, observe the changes in serum metabolites of mice after administration of traditional Chinese medicine Yuanbaocao, and provide a preliminary explanation for the analgesic mechanism. Methods Establish two analgesic models, the hot plate method and the acetic acid writhing method, and use GC/MS to detect and analyze etabolites in mouse serum. After collecting data, PLS-DA method is used to detect and classify endogenous small molecule metabolites in the samples. Results The hot plate analgesic experiment showed that there were extremely significant differences $\left({P<{0.01}}\right)$ in the licking time between the aspirin group, the high and medium dose groups of Yuanbaocao mice, and the model group at 30 and 60 minutes. As the administration time prolonged, Yuanbaocao showed different inhibitory effects on pain symptoms, which was inferred to be related to the high or low blood drug concentration. Compared with the blank group, the number of body twists in the Yuanbaocao group mice decreased significantly $\left({P <{0.01}}\right)$ , indicating that Yuanbaocao has a certain degree of analgesic effect. There are significant differences in the metabolic characteristics of mouse serum, and further screening of metabolic pathways that have a significant impact on data classification identified that taurine, glutathione, arginine and proline, ascorbic acid 5 metabolic pathways are related to pain metabolism. Conclusion The Yuanbaocao group can reverse abnormal changes to a certain extent and has analgesic effects.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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