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Objective To explore and analyze the blood physiological and biochemical indicators of SPF grade SD rats of different ages and genders. Method 80 SPF grade SD rats were studied and randomly divided into four experimental groups at 4, 12, 24, and 48 weeks of age. The male to female ratio in each group was 1:1. All SD rats were analyzed for their blood physiological indicators using the XFA6030 animal blood cell analyzer; apply the Beckman Coulter AU5800 fully automated biochemical analyzer to analyze its blood biochemical indicators. Result Compared with SD rats aged 12, 24, and 48 weeks, SD rats aged 4 weeks showed significant differences in WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT, PCT, BA #, and BA%(P<0.05), and all indicators reached their peak at 24 weeks. Among the gender differences between male and female, SD rats aged 4 weeks showed differences in BA%; 12. There were differences in WBC and RBC between 24 week old SD rats; 24 week old SD rats showed differences in BA%; 48 week old SD rats showed differences in MCH and PCT, and all differences had P values $<{0.05}$ ; Compared with 12,24, and 48 week old SD rats,4-week old SD rats showed significant differences in AST, CHOL, TP, GLU, CRE, ALT, ALP, BUN, and TG (P<0.05). Among the gender differences between male and female, 4-week old SD rats showed significant differences in CRE and ALP; There were differences in AST, CHOL, and BUN among 12 week old SD rats; There were differences in ALP between 12 week old and 24 week old SD rats, and all P values of the differences were<0.05 . Conclusion There are statistical differences in blood physiological and biochemical indicators among SD rats of the same gender but different age groups. Similarly, there are also differences in blood physiological and biochemical indicators among SD rats of the same age group but different genders.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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