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Isolation and identification of miscellaneous bacteria in lactic acid bacteria beverage
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Li-Ling CHEN, Hong-Hu SUN, Jun-Xia LI, Hong-Lin ZHU, Man-Jiang ZHENG, Xiu-Mei LING*
Laboratory Testing | 2024, 2(1) : 123 - 128
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Laboratory Testing | 2024, 2(1): 123-128
Evaluation and Analysis
Isolation and identification of miscellaneous bacteria in lactic acid bacteria beverage
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Li-Ling CHEN, Hong-Hu SUN, Jun-Xia LI, Hong-Lin ZHU, Man-Jiang ZHENG, Xiu-Mei LING*
Affiliations
  • Chengdu Food Inspection and Research Institute Chengdu 610000 China
Published: 2024-01-08
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Objective To separate and identify the miscellaneous bacteria in sterilized lactic acid bacteria beverages provided by an enterprise. Methods The miscellaneous bacteria of lactic acid bacteria beverages were isolated and purified using a variety of medias. The isolated strains were identified at the genus and species levels by analysis of ${16}\mathrm{\;S}$ rRNA sequence and matrix-assisted laser desorption/ionization time of flight mass spectrometry. Results A total of 16 strains were isolated, Among them, 5 strains of Acetobacter, 2 strains of Gluconobacter japonicus, 1 strain of Gluconacetobacter liquefaciens, 1 strain of Kocuria palustris, 1 strain of Streptomyces hydrogenans, 1 strain of Staphylocaccus saprophyticus, 1 strain of Bacillus sp, 1 strain of Novosphingobium aromaticivorans, 1 strain of Brevundimonas vesicularis, 2 strains of Yeast, most of which (n=8, 50%)were identified as Acetobacteraceae. Conclusion Acetobacter, Bacillus, Staphylococcus, Brevundimonas vesicularis, mold, Yeast and other microorganisms were isolated from the bactericidal lactobacillus beverage, most of which belongs to Acetobacteraceae.

lactic acid bacteria beverages  /  isolation  /  identification  /  Acetobacteraceae
Li-Ling CHEN, Hong-Hu SUN, Jun-Xia LI, Hong-Lin ZHU, Man-Jiang ZHENG, Xiu-Mei LING. Isolation and identification of miscellaneous bacteria in lactic acid bacteria beverage[J]. Laboratory Testing, 2024 , 2 (1) : 123 -128 .
Year 2024 volume 2 Issue 1
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  • Online Date:2025-07-29
  • Published:2024-01-08
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Affiliations
    Chengdu Food Inspection and Research Institute Chengdu 610000 China

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*Xiu-Mei LING,Master, Senior Engineer, Chengdu Food Inspection and Research Institute, Chengdu 610000, China. E-mail:
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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