Objective To evaluate the dietary exposure levels of lead, cadmium, arsenic and chromium in Triticum aestivum L. in Anhui Province in 2023 and to evaluate dietary health risks combined with population consumption. Methods A total of 212 Triticum aestivum L. samples collected from Anhui Province in 2023 were analyzed for plumbum, cadmium, arsenic, and chromium content using inductively coupled plasma mass spectrometry (ICP-MS). The contamination status of the 4 kinds of elements was evaluated using the contamination index method. The margin of exposure (MOE) was used for plumbum exposure assessment, while the provisional tolerable monthly intake (PTMI) or benchmark dose level (BMDL) compared with dietary exposure, expressed as the margin of safety (MOS), was applied for cadmium and arsenic exposure assessment. For chromium, the dietary exposure (EXP) was calculated as a percentage of the tolerable daily intake (TDI). Results Among the 212 Triticum aestivum L. samples, As levels did not exceed the standard limits. The exceedance rates for cadmium, plumbum, and chromium were 1.89%, 1.42% and 7.55%, respectively. The single-factor contamination index for all elements was less than 1, indicating safety, while the comprehensive contamination index was 1.737, suggesting mild contamination. The average dietary exposure levels of plumbum, cadmium, and arsenic for residents of different age groups and cities in Anhui Province showed MOE and MOS values greater than 1, and the EXP/TDI percentage for chromium was less than 1. However, for high-exposure P_97.5 populations, including children aged 13-36 months and 7-12 years, the EXP/TDI percentage for chromium exceeded 1. Similarly, in northern Anhui cities such as Fuyang, Huaibei, and Suzhou, the EXP/TDI percentage for Cr in high-exposure P_97.5 populations also exceeded 1. Conclusion The exposure risk levels of plumbum, cadmium, arsenic, and chromium from Triticum aestivum L. consumption among Anhui residents are generally acceptable. However, special attention shall be given to children, adolescents, and high-exposure populations in Fuyang, Huaibei, and Suzhou.

Objective To investigate the pollution of organophosphorus flame retardants (OPFRs) in soil and nuts in the primary Carya illinoensis production areas of Anhui Province, and evaluate the ecological risk and human health risk. Methods A total of 15 soil and nut samples were collected from the main production areas of Carya illinoensis in Anhui Province, and the pollution characteristics of 14 kinds of OPFRs were analyzed. The ecological risk of soil was evaluated using the risk quotient (RQ) method, and the human health risk of consuming nuts was assessed using a health risk assessment model. Results The concentration range of OPFRs in soil samples from Carya illinoensis production areas in Anhui Province was 162.33-394.48 μg/kg, with an average of 305.83 μg/kg. Tris(2-ethylhexyl)phosphate (TEHP) had the highest detection concentration, at 52.62 μg/kg. The concentration range of OPFRs in nut samples was 127.57-310.81 μg/kg, with an average of 223.93 μg/kg. Tris(2-ethylhexyl) phosphate (TEHP) had the highest detection concentration, averaging 46.82 μg/kg. The RQ values of 11 kinds of OPFRs ranged from 3.50×10^-5 to 0.47, with tris(1,3-dichloro-2-propyl) phosphate (TDCIPP), triphenyl phosphate (TPhP), and 2-ethylhexyl diphenyl phosphate (EHDPP) having RQ values between 0.1 and 1.0. The remaining RQ values were all less than 0.1. The health risk assessment of human intake of pecan showed that the hazard quotient (HQ) values of children and adults were both less than 0.1, and the risk of intake of TBEP was higher in children and adults, with the HQ value of adults slightly higher than that of children. Conclusion There is a particular ecological risk of OPFRs in the soil of some production areas of Carya illinoensis in Anhui Province, and the residues of OPFRs in nuts can be ignored in terms of human health risk. This study provides a basis for the safety assessment of novel pollutants in the soil and nuts of Carya illinoensis production areas.

Cyperue esculentust L. oil was a kind of high-quality edible oil rich in monounsaturated fatty acids, protein and other nutrients. This paper reviewed the main extraction technologies of Cyperue esculentust L. oil (supercritical CO₂ extraction, subcritical butane extraction, pressing, leaching, aqueous enzymatic extraction, aqueous extraction, ultrasonic-assisted extraction and microwave-assisted extraction) in recent years. By summarizing and analyzing the characteristics of different extraction technologies in practical operation and their effects on the basic physical and chemical properties, fatty acids, sterols, vitamin E and volatile compounds of Cyperue esculentust L. oil, it was suggested that the development of Cyperue esculentust L. oil in the future could focus on 3 aspects: Optimizing the key machinery and equipment of traditional oil extraction technologies (pressing, aqueous extration and leaching) to improve the quality of oil and reducing the production cost of new oil extraction technologies (aqueous enzymatic extraction, supercritical CO₂ extraction, ultrasonic-assisted extraction, subcritical butane extraction and microwave-assisted extraction) to increase the popularization feasibility, strengthening the cultivation of new varieties of Cyperue esculentust L. oil with high oleic acid and increasing the research and development of its functions, so as to be beneficial to the industrialization of Cyperue esculentust L. oil.

Objective To study the nutritional components of Weiying watermelon foranalyze and evaluate the nutritional quality of Weiying watermelon. Methods Namely “Meidu” watermelon from 2 main production areas of Weiying watermelon (S1 and S2) were used as experimental materials, and the same variety of watermelon from two other production areas (S3 and S4) as controls. The content of sugar, organic acid, amino acid, mineral, soluble solid, β-carotene, lycopene and vitamin C in the watermelons from the 4 production areas were detected and analyzed. Results The watermelon from different producing areas exhibited certain variations in quality characteristics. The sugar components in watermelon was mainly glucose and fructose, organic acids was mainly malic acid and lemon, free amino acids was mainly citrulline, and it also contains abundant mineral elements; the glucose content, sugar-acid ratio and sweetness-sourness ratio in S1 and S2 were higher than those in S3 and S4, while the total acid content was the opposite. In addition, the levels of the citrulline, β-carotene, lycopene and vitamin C in S1 and S2 were also higher than those in S3 and S4, respectively. The comprehensive performance of the nutritional quality of S1 and S2 were superior to that of S3 and S4 based on the principal component analysis. Conclusion Weiying watermelon has better flavor and texture, richer nutritional components, and superior nutritional quality.

Tricholoma matsutake is a high-quality edible wild fungi in China, rich in a variety of nutritional constituents. Research shows that Tricholoma matsutake contains Tricholoma matsutake polysaccharides, Tricholoma matsutake proteins, Tricholoma matsutake polyphenols, sugar alcohols and other nutrients, high nutritional value, delicious taste, with the promotion of intestinal peristalsis, antioxidant, activation of the immune system, anticancer, antihypertension, antidiabetic and other efficacy, is known as “King of Fungi”. The principal production regions of Tricholoma matsutake in China is divided into the southwest region, such as Sichuan Province, Yunnan Province and other places, as well as the northeastern region, such as Heilongjiang Province, Jilin Province. Due to the different genetic backgrounds and growth environments of different production areas, the types and content of nutrients contained in Tricholoma matsutake are also different. Tapping into the nutritional quality characteristics of local Tricholoma matsutake is the basis for developing local specialty Tricholoma matsutake industry and creating regional brands. This paper systematically summarized and compared the main nutritional characteristics of the Tricholoma matsutake produced in the main producing regions of China. In general, the total fat content of the matsutake mushrooms in the producing regions was lower and the protein content was higher, among which the amino acid and crude fiber contents were higher in the southwestern producing region, while the northeastern producing region had lower fat content and higher average protein content. This review will provide new ideas and theoretical basis for the development of specialty food and health food of Tricholoma matsutake in each production area in China.

Objective To develop artificial hypoglycemic rice with leafy grass as a nutritional supplement, optimize its formula, and analyze its inhibitory activity on α-glucosidase and sensory quality. Methods The orthogonal experimental design was used to optimize the formula parameters of artificial rice, with corn amylose and rice starch as the main raw materials and edible leaf grass powder as the functional additive. The inhibitory activity of α-glucosidase was detected by enzyme labeling instrument to evaluate the hypoglycemic effect the samples, and the food sensory analysis was carried out according to the sensory evaluation method. Results The water extract of edible leaf grass showed a high inhibitory rate on α-glucosidase with an inhibitory concentration (IC₅₀) value 1.189 mg/mL, which was close to the inhibitory effect of the positive control acarbose. The optimized formula for producing artificial rice from edible leaf grass performed excellently in sensory evaluation, with good flavor, texture and appearance. Conclusion Edible leaf grass has excellent nutritional value and potential hypoglycemic activity. The artificial hypoglycemic rice develops based on it not only meets the special needs of diabetes patients, but also provides a new choice for the health food market, and has broad application prospects.

Objective To develop a Dioscorea polystachya-based probiotic beverage and assess its shelf life. Methods Using Dioscorea polystachya as the raw material, pH, viable cell count, and sensory evaluation were selected as indicators to screen suitable fermentation strains. The fermentation process was optimized through orthogonal experiments. The changes in viable cell count, sensory quality, soluble solids, pH, and total phenolic content of the beverage were studied during 30 days of storage at different temperatures (4, 25, 35 °C). A shelf life model was established based on the Arrhenius equation and first-order kinetic reaction, which was then used to predict the shelf life of the Dioscorea polystachya probiotic beverage. Results Lactobacillus plantarum, Streptococcus thermophilus and Lactobacillus bulgaricus were selected as the fermentation strains, with a ratio of 1:2:2. The optimal fermentation conditions were 4.5% sucrose, 4% inoculum, 48 hours fermentation time, and a fermentation temperature of 33 °C. The beverage stored at 4 °C showed better stability. The chemical kinetics model predicted the shelf life of the Dioscorea polystachya probiotic beverage to be 37 days under 4 °C storage conditions. Conclusion By optimizing the fermentation process and establishing a shelf life prediction model, this study provides theoretical and technical support for the production and storage of Dioscorea polystachya probiotic beverages, which can maintain good quality for an extended period under low-temperature storage conditions.

Objective To understand the residual levels of chlorpyrifos in vegetables and fruits in Shanghai in 2014—2023, and evaluate the dietary exposure risk of chlorpyrifos in vegetables and fruits. Methods A number of different varieties of vegetables and fruits in Shanghai were inspected and monitored for the detection of chlorpyrifos from 2014 to 2023. Combined with the food consumption data of residents in Shanghai in 2013, dietary exposure risk assessment of chlorpyrifos were conducted by point assessment method. The assessment results were compared with the acute reference dose (ARfD) and acceptable daily intake (ADI) to assess the acute and chronic exposure risk of chlorpyrifos in vegetables and fruits in residents in Shanghai. Results The quantity of vegetables samples was 33796, the detection rate was 0.97%, the average value was 0.00131 mg/kg, and the exceedance rate was 0.46%. The quantity of fruits samples was 9228, the detection rate was 5.26%, the average value was 0.00320 mg/kg, and the exceedance rate was 0.00%. The acute exposure of chlorpyrifos residues ingested through vegetables and fruits were 0.0432 mg/kg bw and 0.0141 mg/kg bw, the ratio of acute exposure to ARfD respectively were 43.19% and 14.15% among the population aged 3 and above. The chronic exposure of chlorpyrifos residues ingested through vegetables and fruits were 5.34×10^-6 mg/kg bw and 8.43×10^-6 mg/kg bw, the ratio of chronic exposure to ADI respectively were 0.053% and 0.084%. Conclusion Both the acute and chronic exposure risks of chlorpyrifos ingested through vegetables and fruits in residents are low in Shanghai.

Objective To investigate the prevalence, virulence phenotypes, and drug resistance phenotype of Vibrio parahaemolyticus in freshwater food, seafood, and ready-to-eat food in Yangzhou. Methods Freshwater food, seafood and ready-to-eat food samples were collected from 9 sampling points in Yangzhou markets for isolation and identification of Vibrio parahaemolyticus. The motility, hemolytic activity, and drug resistance phenotype of the isolates were analyzed. Results In 2023, a total of 289 samples were collected in the Yangzhou market, from which 63 strains of Vibrio parahaemolyticus were isolated, with an isolation rate of 21.80%. Among them, the isolation rate of freshwater food was 45.35% (39/86), seafood 28.75% (23/80), and ready-to-eat samples 0.81% (1/123), indicating a higher isolation rate in freshwater food compared to seafood in the markets of Yangzhou. The proportion of highly motility strains in freshwater food isolates was 30.77%, higher than that of the seafood isolates (17.39%). However, the proportion of strains with high hemolytic activity was 53.85% in freshwater food isolates, slightly lower than the 60.87% in seafood isolates. Antibiotic resistance profiling showed that all Vibrio parahaemolyticus isolates were resistant to ampicillin and cefazolin, while they were resistant to ceftazidime, tetracycline, ciprofloxacin, chloramphenicol, amikacin, meropenem and gentamicin. Conclusion This study highlights a high detection rate of Vibrio parahaemolyticus in Yangzhou markets and reveals differences in virulence and antibiotic resistance among isolates from different sources, offering valuable insights for monitoring and controlling the pathogen.

Objective To investigate the induced expression level and enzymatic properties of recombinant bacterial laccase and to improve the degradation efficiency of sulfadiazine antibiotics by bacterial laccase. Methods In this study, the effects of induction temperature, induction time, and concentration of isopropyl β-D-thiogalactopyranoside (IPTG) on enzyme expression were investigated by one-way experiments. The enzymatic properties of recombinant bacterial laccase were also investigated by both pH and temperature. Results The degradation of sulfadiazine by recombinant bacterial laccase at 24 h was 50%. The optimal conditions for the expression of recombinant bacterial laccase in Escherichia coli BL21 (DE3) were as follows: Induction temperature of 16 ℃, induction time of 16 h, and IPTG concentration of 0.2 mmol/L. The optimal pH was 3, and the stability was best at pH 7, with the enzyme activity reaching 150% in 6 h. The optimal temperature was 80 ℃, and the thermal stability was best at 70 ℃, with the enzyme activity increasing to 120% in 1 h. The half-life was about 6 h. Conclusion This study provides an experimental basis for the application of bacterial laccase in the degradation of sulfadiazine antibiotics and lays down a theoretical basis for the future study of its potential application in the industry.