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Objective To develop a Dioscorea polystachya-based probiotic beverage and assess its shelf life. Methods Using Dioscorea polystachya as the raw material, pH, viable cell count, and sensory evaluation were selected as indicators to screen suitable fermentation strains. The fermentation process was optimized through orthogonal experiments. The changes in viable cell count, sensory quality, soluble solids, pH, and total phenolic content of the beverage were studied during 30 days of storage at different temperatures (4, 25, 35 °C). A shelf life model was established based on the Arrhenius equation and first-order kinetic reaction, which was then used to predict the shelf life of the Dioscorea polystachya probiotic beverage. Results Lactobacillus plantarum, Streptococcus thermophilus and Lactobacillus bulgaricus were selected as the fermentation strains, with a ratio of 1:2:2. The optimal fermentation conditions were 4.5% sucrose, 4% inoculum, 48 hours fermentation time, and a fermentation temperature of 33 °C. The beverage stored at 4 °C showed better stability. The chemical kinetics model predicted the shelf life of the Dioscorea polystachya probiotic beverage to be 37 days under 4 °C storage conditions. Conclusion By optimizing the fermentation process and establishing a shelf life prediction model, this study provides theoretical and technical support for the production and storage of Dioscorea polystachya probiotic beverages, which can maintain good quality for an extended period under low-temperature storage conditions.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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