Objective To establish a method for the determination of biogenic amines in prefabricated food by ultra performance liquid chromatography-tandem mass spectrometry. Methods The samples were extracted with 5% trichloroacetic acid, defatted with n-hexane, and purified using a cation exchange solid-phase extraction cartridge prior to instrumental analysis. The separation was carried out on an ACQUITY UPLC BEH Amide column with gradient elution using a mobile phase made up of pH 4.5, 90 mmol ammonium formate and acetonitrile. Detection was carried out in electrospray ionization-positive ion multiple reaction monitoring mode, and isotope internal standard method was used for quantitation. Results The method demonstrated a good linear standard curve (r≥0.9990); the limits of detection of histamine, tyramine and tryptamine were 0.1 mg/kg, and the limits of detection of putrescine and cadaverine were 1.0 mg/kg; the average recoveries of the 5 kinds of target compounds ranged from 85.7% to 123.4% with relative standard deviations of 2.89%-14.14%. In 201 samples, the detection rates of 5 kinds of biogenic amines were 90.5%. Cadaverine, histamine, putrescine, tyramine and tryptamine were detected, with average content of 2.39, 3.69, 17.86, 9.45 and 0.23 mg/kg. Conclusion This method is rapid, simple, sensitive and accurate, and is sutiable for qualitative and quantitative detection of biogenic amines in prefabricated food.

Objective To investigate the contamination of fusarium and alternaria toxins in wheat and wheat flour in Shaanxi Province. Methods A total of 140 samples of wheat and wheat flour were randomly collected in circulation of 9 cities from 2022 to 2024. Deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), nivalenol (NIV), zearalenone (ZEN), tenuazonic acid (TeA), alternariol (AOH), alternariol monomethyl ether (AME) and tentoxin (TEN) were determined by high performance liquid chromatography-tandem mass spectrometry. Results The fusarium and alternaria toxins were detected in wheat and wheat flour. The detection rates of DON, 3-AcDON, 15-AcDON, NIV, ZEN, TeA, TEN, AME in wheat were 83.3% (50/60), 5.00% (3/60), 10.0% (6/60), 11.6% (7/60), 8.33% (5/60), 100% (60/60), 100% (60/60) and 98.3% (59/60), respectively. The detection rates of DON, TeA, TEN, AME in wheat flour were 87.5% (70/80), 96.2% (77/80), 88.7% (71/80) and 63.7% (51/80), respectively. A strong positive correlation between DON and 15-AcDON content was demonstrated in the positive samples, so was between TeA and TEN content. The DON dietary exposure was 959.8 ng/(kg·bw·d), which was less than the corresponding tolerable daily intake value. The TeA, TEN, AME dietary exposures were 159.2, 18.1 and 2.30 ng/(kg·bw·d), which were below the corresponding threshold of toxicological concern values in wheat flour. Conclusion Wheat and wheat flour in Shaanxi Province are generally contaminated with fusarium and alternaria toxins from 2022 to 2024, with the highest detection rates of TeA and TEN, following by DON. The fusarium and alternaria toxins exposure risk of wheat flour are within an acceptable risk range.

Objective To explore the process of caffeine removal from green tea by supercritical carbon dioxide extraction. Methods Using Wuyuan green tea as raw material, supercritical carbon dioxide extraction was used to remove caffeine from green tea. Taking the caffeine removal rate as the investigation index, the effects of tea moisture content, extraction temperature, pressure, time, concentration and dosage of entrained agent on caffeine removal rate were studied by single factor experiment, and the caffeine removal process was optimized by orthogonal experiment. Results The optimal extraction process of caffeine removal in green tea was tea moisture content 40%, extraction pressure 30 MPa, extraction temperature 55 ℃, extraction time 240 min, concentration of entrainment agent (ethanol) 60%, and dosage of entrainment agent 1.2:1 (m:m). Under these conditions, the caffeine removal rate was 91.2%. Extraction pressure was the most important factor affecting caffeine removal rate, followed by extraction temperature, and extraction time had relatively little effect. Conclusion This study determines the best technology of caffeine removal in green tea by supercritical carbon dioxide extraction, which provides a certain reference for caffeine removal in green tea.

Objective To optimize coffee peel beverage formula by response surface methodology with coffee peel and black tea as the main ingredients. Methods First, the ripe coffee fresh fruits were peeled. After homogenization and filtration, coffee peel liquid was obtained. This liquid was then mixed with black tea liquid, fructose and citric acid for formulation. Single-factor experiments and the response surface methodology were employed to investigate the effects of the addition amounts of coffee peel juice, black tea liquid, fructose, and citric acid on the coffee peel beverage, thereby determining the optimal formulation for it. Results The optimal addition amounts were 28% coffee peel juice, 40% black tea liquid, 5% fructose and 0.05% citric acid. This formulation yielded a product with protein content (5.13±0.34)%, fat content (4.94±0.78)%, dietary fiber (18.43±1.06)%, ash (1.00±0.01)%. The coffee peel juice beverage processed according to this process had a strong aroma, a deep amber color, and a delicious sour and sweet taste. Conclusion The application of response surface methodology to optimize the formula of coffee peel beverage provides another feasible way for the high value utilization of coffee peel and the development of functional products.

Objective To detect and analyze the residue conditions of 57 kinds of pesticide in commercially available fruits in Handan Area from 2020 to 2024 and assess the health risks of pesticide residues for local residents. Methods Followed QuEChERS extraction, samples were analyzed using liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry. Chronic dietary exposure was assessed via a Monte Carlo simulation-based risk model, whereas acute risk was determined by the acute reference dose. Results The p detection rate of pesticide types in 80 fruit samples was 28.1% with an overall item detection rate of 1.12% and overall sample detection rate of 33.8% in 80 fruit samples. Dimethoate residues exceeded national standards in 6 cherry and 3 jujube samples. Two or more pesticides were detected in 16.2% of the samples. No highly or extremely toxic pesticides were detected. The chronic dietary intake risk of dimethoate was 1.81, exceeded the safe range, while the chronic dietary intake risk of other pesticides were within acceptable limits. The hazard index value was 1.87, exceeded the safe range. Acute dietary risks for all detected pesticides were within safe ranges. Dimethoate and endosulfan were identified as higher-risk pesticides in both acute and chronic assessments. Conclusion The dietary intake risk of pesticide residues in Handan City (2020—2024) is generally within safe limits. However, there are cases of dimethoate exceeding the standard and multiple pesticides detected in the same sample. Dimethoate posed chronic dietary intake risk and the relevant authorities need to maintain ongoing attention.

Objective To investigate the residues characteristics of veterinary drug in Gallus gallus domesticus from Hubei Province and assess the dietary exposure risks. Methods The 34 types of veterinary drug residues were detected in 100 randomly collected samples of Gallus gallus domesticus from farms, agricultural markets, wholesale markets and slaughterhouses in Hubei Province in 2024 by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The dietary exposure risk assessment of detected veterinary drug residues was conducted using the veterinary drug residue risk ranking matrix of the United Kingdom Veterinary Drug Residue Committee and the index of food safety (IFS) method. Results The 12 kinds of veterinary drugs were detected in the samples, with a residue detection rate of 49%. Among these, 2 kinds of drugs were prohibited for use in food animals, and 1 kind of drug was approved for therapeutic use but prohibited from detection in animal-derived foods. The risk assessment revealed that the IFS values of all detected veterinary drugs were significantly less than 1, indicating a low dietary exposure risk from residues. Conclusion This suggests that the quality and safety of Gallus gallus domesticus in Hubei Province are satisfactory, with minimal health risks posed by residual veterinary drugs to humans.

Many foodborne Chinese herbs exhibit significant efficacy in sedation and hypnotic effects, with the advantages of safety, mildness, and convenience. These properties have garnered widespread attention from individuals suffering from insomnia, scientific researchers, and relevant practitioners. Such herbs are predominantly utilized in health foods and ordinary foods. In the realm of health foods aimed at aiding sleep, dosage forms are diverse, and ingredients such as Semen Ziziphi Spinosae, Ganoderma lucidum, and Schisandra chinensis are frequently employed. Within the domain of ordinary foods, a variety of products including medicinal diets, beverages, and snacks are involved, showcasing diverse product forms and production processes. Regarding research advancements, the effective dosages of different food-derived Chinese herbs medicines for improving sleep vary, and their sleep-aid components encompass terpenoids, alkaloids, flavonoids and other constituents. The mechanisms of action span neurotransmitter regulation, signaling pathway modulation, gut microbiota improvement, antioxidative and anti-inflammatory activity. Nevertheless, there remain deficiencies in the research and product development of foodborne Chinese herbal medicines for sleep aid. This paper systematically summarized the applications and usage rules of foodborne Chinese herbal medicines in sleep aid foods, reviewed modern research on foodborne Chinese herbal medicines and sleep aids, and elucidated the efficacious components and mechanisms of foodborne Chinese herbal medicines for sleep enhancement, thereby providing valuable references for the research and development of sleep aid products based on foodborne Chinese herbal medicines.

The frequent occurrence of adulteration in meat products not only affects people's quality of life but also restricts the healthy development of the industry. The technology of identifying adulteration in meat products has become a research hotspot. Fast, accurate, reliable, and simple meat ingredient detection technology is an effective means of identifying adulteration in meat products This article reviewed the principles, advantages, disadvantages and application research of various meat source component detection technologies that had been extensively studied, including spectroscopic technology, chromatographic and mass spectrometry analysis technology, immunoassay technology and isothermal amplification analysis technology. It also looked forward to the future research directions of these technologies, in order to provide theoretical basis and reference ideas for the research of meat product adulteration identification technology.

Objective To optimize the ultrasonic extraction process of dihydromyricetin from Ampelopsis grossedentata using artificial neural networks combined with response surface methodology. Methods The stems and leaves of Ampelopsis grossedentata were used as the research material. An ultrasonic extraction system for dihydromyricetin was established, and the process parameters were systematically optimized using a combination of single-factor experiments, response surface methodology and artificial neural network models optimized by genetic algorithms. The extraction yields of dihydromyricetin from different parts of Ampelopsis grossedentata were then analyzed under optimal conditions. Results The artificial neural network model exhibited superior accuracy and predictive capability in comparison to the response surface methodology. The optimal extraction conditions were determined to be an ultrasonic power of 360 W, a temperature of 42 ℃, a liquid-to-solid ratio of 20:1 (mL:g), and an extraction time of 35 min. Under these conditions, the actual extraction yield of dihydromyricetin was (39.83±0.01)%, with a relative error of only 0.36% compared to the artificial neural network-predicted value of 40.19%. Furthermore, the extraction yield of dihydromyricetin from various parts of Ampelopsis grossedentata under optimal ultrasonic conditions followed the sequence: Stems and leaves of Ampelopsis grossedentata>branches of Ampelopsis grossedentata>pruned branches of Ampelopsis grossedentata. Conclusion This study successfully optimizes the ultrasonic extraction process to enhance the extraction efficiency of dihydromyricetin from Ampelopsis grossedentata and reveals significant differences in dihydromyricetin extraction yields among different parts of the plant.

Objective To obtain excellent lactic acid bacteria with bacteriostatic activity from fermented bamboo shoots and analyze their antibacterial substances. Methods Using Liuzhou traditional fermented bamboo shoots as raw materials, Staphylococcus aureus and Escherichia coli as indicator bacteria, and inhibition zone diameter as evaluation indicator, the Oxford Cup method was used to screen lactic acid bacteria with higher antibacterial properties. The strain was identified by morphological observation and molecular biological technique. Finally, the effects of pH, temperature and enzyme treatment on the antibacterial activity of the fermentation supernatant of the strain were analyzed. Results A total of 26 strains of lactic acid bacteria were isolated from Liuzhou traditional fermented bamboo shoots, among which 10 strains had antibacterial activity against Staphylococcus aureus and Escherichia coli, and strain LZ-6 had the strongest antibacterial activity, with a diameter of more than 15 mm for the inhibition zone. The strain LZ-6 was identified as Limosilactobacillus fermentum. The antibacterial substances produced by strain LZ-6 exhibit good antibacterial activity only under acidic conditions and had good thermal stability. After protease treatment, there was no significant change in antibacterial activity (P>0.05), indicated that the protein in the fermentation supernatant had no antibacterial effect. Conclusion A fermented lactobacillus LZ-6 with good antibacterial activity is screened from Liuzhou traditional fermented bamboo shoots. Its antibacterial substances are organic acids with stable activity and are not affected by temperature and enzymes. The lactic acid bacteria derived from fermented bamboo shoots obtained in this study can be used in the fields of sour bamboo shoot fermentation and biological preservatives, and lay the foundation for the separation, identification and mechanism research of antibacterial substances.