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Objective To establish a method for the determination of biogenic amines in prefabricated food by ultra performance liquid chromatography-tandem mass spectrometry. Methods The samples were extracted with 5% trichloroacetic acid, defatted with n-hexane, and purified using a cation exchange solid-phase extraction cartridge prior to instrumental analysis. The separation was carried out on an ACQUITY UPLC BEH Amide column with gradient elution using a mobile phase made up of pH 4.5, 90 mmol ammonium formate and acetonitrile. Detection was carried out in electrospray ionization-positive ion multiple reaction monitoring mode, and isotope internal standard method was used for quantitation. Results The method demonstrated a good linear standard curve (r≥0.9990); the limits of detection of histamine, tyramine and tryptamine were 0.1 mg/kg, and the limits of detection of putrescine and cadaverine were 1.0 mg/kg; the average recoveries of the 5 kinds of target compounds ranged from 85.7% to 123.4% with relative standard deviations of 2.89%-14.14%. In 201 samples, the detection rates of 5 kinds of biogenic amines were 90.5%. Cadaverine, histamine, putrescine, tyramine and tryptamine were detected, with average content of 2.39, 3.69, 17.86, 9.45 and 0.23 mg/kg. Conclusion This method is rapid, simple, sensitive and accurate, and is sutiable for qualitative and quantitative detection of biogenic amines in prefabricated food.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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