To explore the pharmacological effects of water extract of Ficus hirta on enhancing immunity and improving gastrointestinal function.

Sixty healthy male Kunming (KM) mice, weighing 18-22 g, were randomly divided into 6 groups: normal group, model group, positive (Thymus-intestinal peptide) group, low-, medium-, and high-dose groups of the test drug, with 10 mice in each group. Except for the normal group, the other groups were given intraperitoneal injection of cyclophosphamide combined with 5-fluorouracil to prepare mice models of immunosuppression and gastrointestinal dysfunction. The normal and model groups were given distilled water, and the positive group and the test drug groups were given the corresponding drugs for 7 consecutive days. The intestinal charcoal transit rate, blood cell count, serum inflammatory cytokines and immune protein levels of the mice were detected, and the pathological damage of intestinal and spleen tissues was observed, and pathological injury of intestinal and spleen tissues were observed.

Compared with the model group, the water extract of Ficus hirta significantly significantly increased the levels of IgA, IgE, IgM, IL-2, and IFN-γ in the plasma supernatant of mice and increased the spleen index, increased the intestinal charcoal transit rate and the number of red blood cells in the blood of mice, and alleviated the pathological injury degree of intestinal and spleen tissues of mice, and to some extent alleviated the immunosuppression induced by cyclophosphamide combined with 5-Fu in mice induced by cyclophosphamide combined with 5-Fu in mice.

This study confirmed that the water extract of Ficus hirta has a promoting effect on enhancing immunity and improving gastrointestinal peristalsis, and provides data reference for the development and utilization of Ficus hirta resources.

To explore the effects of Chuanxiong broken-wall decoction pieces (CXBDP) on acute and long-term toxicity in SD rats, and compare them with traditional Chuanxiong decoction pieces (CXDP), so as to provide more data support for clinical medication.

Acute toxicity: SD rats were given a single oral dose of CXBDP 10.08 g·kg^-1 and CXDP 10.08 g·kg^-1, and then observed for 2 weeks.Long-term toxicity: SD rats were orally given CXBDP 0.54, 1.35, 3.78 g·kg^-1, CXDP 0.54, 3.78 g·kg^-1 for 4 weeks, and then allowed to recover for 4 weeks after drug withdrawal. The effects of CXBDP and CXDP on rats' clinical observation, body weight, food intake, hematology, blood biochemistry, organ weight, organ coefficient and histopathological indicators were investigated.

Acute toxicity: no obvious toxic reactions was observed after single oral administration of CXBDP 10.08 g·kg^-1 (maximum dose) and CXDP 10.08 g·kg^-1. Long-term toxicity: no obvious toxic reactions was observed in SD rats after continuous oral administration of CXBDP (0.54, 1.35, and 3.78 g·kg^-1) and CXDP (0.54 and 3.78 g·kg^-1) for 4 weeks, as well as during the 4-week recovery period after drug withdrawal.

There were no statistically significant difference in safety between CXBDP and traditional CXDP in SD rats.

Cancer-related fatigue (CRF) is one of the most common symptoms in cancer patients. In this study, CRF models of Lewis lung cancer, CT-26 colon cancer and WEHI-3 leukemia mice were established to investigate the dynamic characteristics of CRF and explore evaluation indicators for drug treatment of CRF.

C57BL/6 mice and BALB/c mice were inoculated with Lewis lung cancer cells, CT-26 colon cancer cells and WEHI-3 leukemia cells to induce CRF models. The rotarod test was employed to assess changes in the exercise endurance of the mice following model induction. ELISA was used to detect the changes in the contents of urea nitrogen (BUN), creatine kinase (CK), erythropoietin (EPO), interleukin-1 (IL-1), tumor necrosis factor-α (TNF-α), and high-sensitivity C-reactive protein (hs-CRP) in the serum of mice post-modeling, as well as the levels of superoxide dismutase (SOD) and lactate dehydrogenase (LDH) in skeletal muscle.

All three types of model mice showed symptoms such as fluffy coat, hunched back, reduced activity and slow movement after modeling. Their exercise endurance was significantly reduced. The decrease in exercise endurance among Lewis lung cancer, CT-26 colon cancer and WEHI-3 leukemia model mice increased during the later stage of tumor development. The BUN content in serum decreased significantly on days 17, 28 and 19 post-modeling respectively. Meanwhile the CK content increased significantly. TNF-α levels in serum from the WEHI-3 leukemia model mice significantly increased on day 19 post-modeling. The LDH content within skeletal muscle from CT-26 colon cancer model mice significantly decreased on day 28 post-modeling, and the contents of IL-1, TNF-α and hs-CRP in the serum showed an increasing trend.

Mouse models of Lewis lung cancer, CT-26 colon cancer and WEHI-3 leukemia can be utilized to observe fatigue behavior induced by tumors. Changes in rotarod test time as well as serum CK, BUN, IL-1, TNF-α, hs-CRP and skeletal muscle LDH may serve effectively as evaluation indicators for the efficacy of CRF therapeutic drugs in non-clinical studies.

To develop a dual-crosslinked hydrogel loaded with berberine hydrochloride and to evaluate its physicochemical properties and drug release behavior.

Soluble silk fibroin with high resolubility rate was prepared by autoclaving combined with freeze-drying. A stable silk fibroin-tannic acid hydrogel matrix was established by optimizing the concentration and ratio of silk fibroin to tannic acid. Glucono-δ-lactone (GDL) was then introduced to modulate the gelation process, thereby constructing a silk fibroin-tannic acid/sodium alginate-Ca²⁺ dual-crosslinked hydrogel dual-crosslinked hydrogel loaded with berberine hydrochloride. The in vitro drug release profiles were investigated in buffers at different pH values.

The optimal mass concentrations of silk fibroin and tannic acid were 4% and 0.25%, respectively, under which a stable hydrogel was formed. When 0.32 g of GDL was added, the dual-crosslinked hydrogel formed rapidly and maintained color stability at different temperatures. The hydrogel exhibited injectability, and the release rate of its loaded berberine hydrochloride was significantly faster at pH 7.4 than at pH 5.5.

The as-prepared berberine hydrochloride-loaded dual-crosslinked hydrogel possesses both injectability and pH responsiveness, the developed hydrogel can provide a scientific basis for the development of localized drug delivery systems and novel dressings for chronic wounds.

To explore the global research progress and future trends of Morinda officinalis How. (Bajitian) from 2000 to 2024.

Using CiteSpace software combined with bibliometric methods, relevant Chinese and English literature on Bajitian were collected from the China National Knowledge Infrastructure (CNKI), Wanfang Data Knowledge Service Platform, VIP Biomedical Database, and Web of Science. Duplicate literatures were eliminated with NoteExpress software, and visual analysis and knowledge graph construction were performed on the publication countries, publication volumes, authors, institutions, keywords, etc., using CiteSpace software.

A total of 2 712 Chinese articles and 374 English articles were included. From 2000 to 2024, the publication volume of research related to Bajitian had steadily increased, and both domestic and international research entered a rapid development stage in 2009. China was the leading country in Bajitian research, and the core domestic research institution was Guangzhou University of Chinese Medicine. Research on Bajitian covered various aspects such as content determination, pharmacological effects, mechanisms of action, and processing methods. Internationally, the focus had been primarily on the pharmacological effects and active ingredients of Bajitian extracts.

Research on Bajitian had entered a diversified development stage, covering multiple aspects including medication rules, chemical constituents, content determination, fingerprint spectrum, famous TCM physician experience, and clinical application. After 2020, studies using data mining, network pharmacology, and other technologies for molecular docking and mechanisms of action have become a research hotspot, mainly focusing on anti-inflammation, anti-depression, anti-osteoporosis, anti-oxidation, and the treatment of ovarian and testicular dysfunction.

To systematically elucidate the molecular mechanism of Herba Abri intervened liver fibrosis via regulating ferroptosis using network pharmacology, molecular docking, bioinformatics, and machine learning approaches.

Active components of Abri Herba were screened via the TCMSP database. Liver fibrosis and ferroptosis-related genes were retrieved from GEO and FerrDb databases. R language was employed for batch correction, differential analysis, and weighted gene co-expression network analysis (WGCNA). Core genes were identified by intersecting targets of Abri Herba, liver fibrosis, and ferroptosis. Protein-protein interaction (PPI) networks and "drug-component-target-disease" network were constructed using STRING and Cytoscape. Functional enrichment analyses (GO and KEGG) were conducted to identify core pathways. Feature genes were screened via random forest and XGBoost algorithms, validated by Mendelian randomization (MR) analysis and receiver operating characteristic (ROC) curves, followed by molecular docking.

(1) Twelve active components and 301 predicted targets of Herba Abri were identified. Batch correction and principal component analysis (PCA) integrated 17 731 liver fibrosis-related genes and 396 ferroptosis-related genes, obtaining 27 intersection targets. (2) GO analysis revealed enrichment in oxidative stress response and nuclear receptor activity. KEGG pathways were enriched in cancer metabolism (hsa05207) and PPAR signaling (hsa03320). (3) Machine learning (random forest and XGBoost) prioritized signature genes AR and FABP4. (4) Validation via ROC curves demonstrated AUC values were 0.591 for AR and 0.623 for FABP4, supported by MR analysis. (5) Molecular docking confirmed stable interactions between AR, FABP4 and active components of Herba Abri.

Abri Herba may inhibit ferroptosis in liver fibrosis by regulating oxidative stress and lipid metabolism pathways to inhibit ferroptosis and thereby suppress liver fibrosis through AR and FABP4, providing novel evidence for the multi-target therapeutic potential of traditional Chinese medicine.

So as to provide a reference for optimizing the pharmaceutical care model for resident pharmacists in obstetrics and gynecology, and to evaluate its impact on rational drug use and healthcare quality. This aims to provide a reference for optimizing the pharmaceutical care model of resident pharmacists in these departments.

A retrospective study was conducted. Data on clinical pharmaceutical services from the Obstetrics and Gynecology Department of a hospital were compared before (January 2024-December 2024, control group) and after (January 2025-December 2025, intervention group) the implementation of the resident pharmacist model. Core indicators included the Antibiotic Use Density (AUD), rational use rate of antibiotics for type II incisions, number of reported adverse drug reactions (ADRs), and drug proportion control and medication cost control. This was combined with a comparative analysis of online and offline service data from the pregnancy and lactation pharmacy clinic.

Following implementation, the pregnancy and lactation pharmacy clinic completed 568 offline consultations and 1 121 online consultations. Clinical affinity was significantly improved (P<0.01). Core rational medication use indicators showed marked improvement: AUD decreased by 13.5% in obstetrics and 23.7% in gynecology (both P<0.01). The rational use rate of antibiotics for type II incisions improved significantly (P<0.01). The acceptance rate of antibiotic consultation recommendations reached 100% in both departments. ADR reporting increased. The drug proportion and average per capita drug cost were significantly decreased. The intravenous infusion rate and average length of stay in gynecology were both significantly decreased (P<0.01). After the implementation of fees for the pharmacy clinic, the pregnancy and lactation clinic conducted 392 fee-based consultations, averaging 28 per month, exceeding the average monthly number of consultations before charging. Following the implementation of the inpatient consultation fee (clinical pharmacy) policy, 732 cases of medication therapy management were conducted.

The collaborative model integrating the pharmacy clinic with resident pharmacists effectively addresses clinical pain points through medication risk assessment and medication optimization. It enhances physician-pharmacist collaboration, significantly improves rational drug use indicators, and achieves tripartite collaboration among physicians, pharmacists, and patients. This model provides a innovative solution for medication safety in special populations and which is worthy of promotion and application.

To provide reference for guiding individualized medication in epilepsy patients by retrospectively analyzing the therapeutic drug monitoring (TDM) of new antiseizure medications in the neurology department of a hospital.

Clinical data were collected from outpatients and inpatients in the neurology department (including pediatric neurology, neurology, and neurosurgery) of the hospital from January 2022 to August 2024, who underwent blood concentration monitoring of two new antiseizure medications, namely oxcarbazepine (OXC) and levetiracetam (LEV). The data included gender, age, antiseizure medication regimens, blood concentration measurements, clinical efficacy, and adverse drug reactions (ADRs). Statistical analysis was performed to compare whether there were differences in blood concentration levels among different age groups, genders, and combination therapies, as well as whether differences existed in clinical efficacy and ADR incidence across different blood concentration ranges.

Among the 414 TDM records, 206 cases were for OXC and 208 cases for LEV. Blood concentrations within the reference range were observed in 114 cases (69.9%) for OXC and 86 cases (41.35%) for LEV. A statistically significant difference in LEV blood concentrations was found among different age groups (P<0.05). The total incidence of ADRs in the OXC group (13.11%) was higher than that in the LEV group (0.27%), and ADR incidence in both groups was significantly correlated with blood concentration (P<0.05). However, no significant differences in blood concentrations were observed based on gender or combination therapy for either OXC or LEV (P>0.05). Additionally, no significant differences in clinical efficacy were found among different blood concentration groups (P>0.05).

Although the two new antiseizure medications, OXC and LEV, didn't show a significant positive correlation between blood concentration and clinical efficacy in the TDM process, their drug exposure levels are closely associated with the risk of ADRs. Blood concentration monitoring can provide valuable guidance for rational clinical drug use, ensuring safe and effective treatment for patients.

To explore the therapeutic effect of Huangqi Guizhi Wuwu decoction on oxaliplatin-related peripheral neurotoxicity in patients with colorectal cancer receiving adjuvant chemotherapy.

A total of 42 colorectal cancer patients who received mFOLFOX6 adjuvant chemotherapy in a hospital from March 2022 to December 2024 were enrolled in this study. They were randomly divided into two groups (n=21 each). The control group was treated with chemotherapy alone, while the experimental group received Huangqi Guizhi Wuwu decoction for 4 cycles concurrently with chemotherapy. The CTCAE V4.0 standard was used to evaluate oxaliplatin-related peripheral neurotoxicity, and the primary study endpoint was the incidence of grade 3 and above peripheral neurotoxicity.

The incidences of grade 3 or higher peripheral paresthesia in the experimental group and the control group were 23.8% vs. 57.1% (P=0.03). The incidences of grade 3 and above peripheral sensory neuropathy were 19.1% vs. 47.6% (P=0.04), with statistically significant differences observed between the two groups.

Taking Huangqi Guizhi Wuwu decoction simultaneously during chemotherapy can effectively prevent and treat oxaliplatin-induced peripheral paresthesia and peripheral sensory neuropathy.

To systematically elucidate the multidimensional mechanisms of vitamin D in wound healing, thereby providing scientific evidence for clinical treatment and offering new directions for wound repair research.

The regulatory effects of vitamin D were analyzed on pathways including VDR, calcium signalling, anti-inflammation, TGF-β/Smad, Hippo, skin barrier-related signaling pathways, PI3K/AKT/HIF-1α, and Notch during different stages of wound healing.

Vitamin D exerted its effects on key aspects of wound healing-including cell proliferation, migration, differentiation, extracellular matrix synthesis and remodelling, inflammatory response, immune regulation, and angiogenesis-by regulating the aforementioned pathways. These pathways intertwined to form a regulatory network that promoted wound healing.

Vitamin D demonstrates significant potential in research into wound healing mechanisms, with its multi-pathway regulatory mechanisms providing multidimensional support for wound repair. Future research should further explore interactions between pathways, carry out clinical trials in humans, and investigate other relevant pathways to advance clinical applications.