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Cancer-related fatigue (CRF) is one of the most common symptoms in cancer patients. In this study, CRF models of Lewis lung cancer, CT-26 colon cancer and WEHI-3 leukemia mice were established to investigate the dynamic characteristics of CRF and explore evaluation indicators for drug treatment of CRF.
C57BL/6 mice and BALB/c mice were inoculated with Lewis lung cancer cells, CT-26 colon cancer cells and WEHI-3 leukemia cells to induce CRF models. The rotarod test was employed to assess changes in the exercise endurance of the mice following model induction. ELISA was used to detect the changes in the contents of urea nitrogen (BUN), creatine kinase (CK), erythropoietin (EPO), interleukin-1 (IL-1), tumor necrosis factor-α (TNF-α), and high-sensitivity C-reactive protein (hs-CRP) in the serum of mice post-modeling, as well as the levels of superoxide dismutase (SOD) and lactate dehydrogenase (LDH) in skeletal muscle.
All three types of model mice showed symptoms such as fluffy coat, hunched back, reduced activity and slow movement after modeling. Their exercise endurance was significantly reduced. The decrease in exercise endurance among Lewis lung cancer, CT-26 colon cancer and WEHI-3 leukemia model mice increased during the later stage of tumor development. The BUN content in serum decreased significantly on days 17, 28 and 19 post-modeling respectively. Meanwhile the CK content increased significantly. TNF-α levels in serum from the WEHI-3 leukemia model mice significantly increased on day 19 post-modeling. The LDH content within skeletal muscle from CT-26 colon cancer model mice significantly decreased on day 28 post-modeling, and the contents of IL-1, TNF-α and hs-CRP in the serum showed an increasing trend.
Mouse models of Lewis lung cancer, CT-26 colon cancer and WEHI-3 leukemia can be utilized to observe fatigue behavior induced by tumors. Changes in rotarod test time as well as serum CK, BUN, IL-1, TNF-α, hs-CRP and skeletal muscle LDH may serve effectively as evaluation indicators for the efficacy of CRF therapeutic drugs in non-clinical studies.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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