To optimize the fermentation conditions of Fupenzi(Rubi Fructus)by Lactobacillus plantarum using Fupenzi(Rubi Fructus)as the raw material.
Taking the ratio of material to liquid,inoculation amount,sugar addition amount and fermentation time as the investigation factors,and the contents of total flavonoids and total phenols as the evaluation indexes,the single factor and orthogonal experiments were designed to screen the optimum process conditions for the fermentation of Fupenzi(Rubi Fructus)by Lactobacillus plantarum,and the characteristic volatile flavor substances before and after fermentation were analyzed.
The optimum conditions for the fermentation of Fupenzi(Rubi Fructus)by Lactobacillus plantarum were as follows:solid-liquid ratio 1:25 g/mL,inoculation amount8%,sugar addition amount2%,fermentation time 24 h. A total of 52 volatile flavor substances were detected in the samples before and after fermentation,including 8 type of alcohols,4 type of ketones,12 type of aldehydes,7 type of acids,7 type of esters,6 type of alkanes,3 type of phenols and 6 type of other substances.
Fermentation can effectively improve the flavor and nutritional value of Fupenzi(Rubi Fructus).
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |