Full
Outline
To analyze the effect of ursolic acid on the expressions of long chain non coding RNA（lncRNA）-LINC01089 and its targetgenes in papillary thyroid carcinoma（PTC）.
LINC01089 was a differentially expressed lncRNA that was previously screened and verified by transcriptome sequencing in the early stage. TPC-1 cells and normal human thyroid cells（Nthy-ori3-1）were routinely cultured in vitro and were divided into normal human thyroid cell group，control group and UA groups（10，20，40 μmol·L-1）. The survival rate of TPC-1 cells in each group was detected by CCK-8 assays. Scratch and Transwell experiments were conducted to investigate the effects of different concentrations of ursolic acid on the horizontal，vertical migration and invasion of TPC-1 cells. RT qPCRmethod was used to detect the relative expression level of LINC01089 in TPC-1 cells and the intervention effect of ursolic acid. RT-qPCR and Western blot were used to detect the mRNA and protein expression levels of target genes Ras Homolog Family Member F（RHOF）and 4-Hydroxyphenylpyruvate Dioxygenase（HPD）as well as the intervention effectof ursolic acid.
Different concentrations of UA（10，20，40 and 80 μmol·L-1）could inhibit the proliferation of TPC-1 cells for different periods of time（12～48 hours）. When the concentration of ursolic acid was 10～40 μmol·L-1，the inhibitory effect of the cells gradually increased with increasing of concentration and prolongation of action time within 12 and 24 hours（P<0.05）. After treating TPC-1 cells with ursolic acid for 24 hours，the cell proliferation was slow，and as the concentration of ursolic acid（10，20 and 40 μmol·L-1）increased，morphological changed in cell apoptosis. Compared with the control group，the ursolic acid（10，20，40 μmol·L-1）groups inhibited the migration and invasion of TPC-1 cells in a significant concentration dependent manner（P<0.01）. Ursolic acid can up-regulate the expression of LINC01089，and down-regulate themRNA and protein expression levels of RHOF and HPD.
Ursolic acid has a certain inhibitory effect on thyroid papillary carcinoma cells，and its mechanism may be related with the LINC01089 and its target gene RHOF.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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