Full
Outline
To explore the effect of circ-0002133/miR-182/Bcl2L12 axis on cell apoptosis and the occurrence and development of hepatocellular carcinoma（HCC）and the intervention of gypenosides（GPs）.
The relevant data of liver cancer were obtained from TCGA database，and the difference analysis，survival analysis，gene expression and clinical correlation analysis of Bcl2 like protein 12（Bcl2L12）were performed. Circ-0002133miRNA and miR-182，which were associated with circAtlas and circRNADisease and were able to establish ceRNA networks with BCL2L12，were respectively predicted using miRanda and targetscan databases. The clinical specimens were verified by qRT-PCR. HepG2 hepatoma cells were then interfered with by GPs，the inhibition rate of GPs on HepG2 cells was detected by CCK-8 method，and the migration ability of HepG2 cells was observed by scratch test. The mRNA expressions of Caspase-3，Caspase-7 and Bcl2L12 were detected by qRT-PCR. The expressions of Caspase-7，Caspase-3 and Bcl2L12 were detected by Western Blot.
The results of TCGA database showed that the expression of Bcl2L12 was significantly increased in LIHCtissues，and high Bcl2L12 was associated with abnormal age，sex and TNM stage. The survival time of high Bcl2L12 HCC patients was shorter than that of low Bcl2L12 HCC patients. Compared with paracuncinoma group，circ-0002133 mRNA decreased（P<0.01），while miR-182 and Bcl2L12 mRNA increased（P<0.01）. The scratch test results showed that compared with the control group，GPs group had obvious inhibitory effect on HepG2 hepatocellular carcinoma cells. Compared with those of the control group，the mRNA levels of Caspase-7 and Caspase-3 in GPs group were increased（P<0.01），and the mRNA levels of Bcl2L12 were decreased（P<0.01）. Compared with those of the control group，the protein levels of Caspase-7 and Caspase-3 in GPs group were increased（P<0.01），and the protein levels of Bcl2L12 were decreased（P<0.01）.
GPs can inhibit the proliferation of hepatoma cells，and the mechanism of action may be related to the apoptosis pathway mediated by circ-0002133/miR-182/Bcl2L12 axis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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