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Outline
This study aimed to investigate the ameliorating effect of Qinjiao Wuwei Formula（秦艽五味方，QJF）on skin lesions in a mouse model of 2，4-dinitrofluorobenzene（DNFB）-induced atopic dermatitis（AD）-like mice and its mechanism.
The DNFB-induced mouse AD-like model was established，and the mice were randomly divided into blank control，model（DNFB），positive control（dexamethasone，DXMS）and QJF groups. The severity of skin lesions was evaluated using the Scoring Atopic Dermatitis Index（SCORAD），and the histomorphological changes and epidermal thickness of the lesions were observed under light microscopy. The number of mast cells was detected by toluidine blue staining，the expression of filaggrin（FLG）in skin lesions was detected by immunohistochemistry，and the expression of serum IgE was detected by ELISA. The proportion of Th1/Th2 cells in the spleen and lymph nodes，the proportion of Th17 cells in lymph nodes were tested by flow cytometry，and the differential expressions of inflammatory factors in serum were detected by high-throughput liquid-phase protein chip technology.
Compared with the model group，the QJFgroup had significantly reduced SCORAD scores and scratching index，thinner skin lesions，and a significant decrease in the number of mast cells in the lesions. The positive expression of FLG was restored，and the expression of IgE was reduced. The ratio of Th1/Th2 in the spleen and lymph nodes increased，while the proportion of Th17 cells in the lymph nodes was decreased. Furthermore，inflammatory cytokines in serum were all decreased after treatment with QJF，with the most significant regulatory effect on the expressions of Th2-related cytokines.
QJF can effectively improve the skin lesions of DNFB-induced AD-like mice while regulating the inflammatory response of Th2-related cytokines.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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