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Outline
This study aimed to elucidate themechanism of the combined use of Yinyanghuo（Epimedii Folium）and Nluvuzhenzi（Ligustrum lucidum Fructus）in enhancing the suppression of endogenous glucocorticoids（GC）in asthmatic rats.
Male SD ratswere randomly divided into five groups：normal，model，Yinyanghuo，luvyuzhenziand compatibility groups. An asthmatic ratmodelwas established using sensitization and stimulation with ovalbumin，followed by a two-week drug treatment via gavage. The serum levels of corticotropin releasing hormone（CRH），adrenocorticotropic hormone（ACTH）and corticosterone（CORT）were assessed using enzyme immunoassay. Targeted metabolomics was used to detect endogenous glucocorticoid（GC）metabolism levels in urine. Immunohistochemical staining was employed to identify protein markers of pulmonary hydroxysteroid dehydrogenase 11β1（HSD11B1），hydroxysteroid dehydrogenase 11β2（HSD11B2）and the expressions of cytochrome P450 family 11 subfamily Bmember 1（CYP11B1）and steroid generation factor 1（SF1）in the adrenal gland. Western blotting was used to ascertain the protein expressions of CYP11B1，SF1，HSD11B1 and HSD11B2 in liver tissue.
Yinyanghuo（Epimedii Folium）and Nluvuzhenzi（Ligustrum lucidum Fructus）alone or in combination can elevate the levels of serum CRH，ACTH and CORT，ameliorate urinary endogenous GC metabolic disorders，up-regulate the expressions of HSD11B1 and HSD11B2 proteins in lung tissue，CYP11B1 and SF1 proteins in the adrenal gland，and the protein expressions of HSD11B2 and CYP11B1 in liver tissue of the model rats.
The effects of Yinyanghuo（Epimedii Folium）and Nluvuzhenzi（Ligustrum lucidum Fructus）in the treatment of asthma are associated with the regulation of hypothalamic-pituitary-adrenal axis inhibition，the modulation of key factors in the GC synthesis ormetabolization pathway，the up-regulation of endogenous GC levels，and the improvement of endogenous GC disorders.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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