Full
Outline
Using Box-Behnken design response surfacemethodology to optimize the preparation process parameters of magnololwhey protein isolate nanoparticles（MAG-WPINPs），and to characterize their formulation and evaluate their antioxidant and antibacterialeffects.
MAG-WPI-NPswere prepared using a desolvation method，with encapsulation efficiency as the evaluation index. The preparation process parameters were optimized using a single factor combined with Box-Behnken design response surface methodology. The optimized MAG-WPI-NPs were characterized by their morphology，particle size，potential and other physicochemical properties. The free radical scavenging ability of 1，1-diphenyl-2-trinitrophenyl hydrazine（DPPH）and its ability to inhibit Escherichia coli were evaluated.
The optimal preparation process parameters included a volume ratio of12：1 for anti solvent/solvent，a feed ratio of12：1 for whey protein/magnolol，and a probe time of 1.5 minutes. The prepared MAG-WPI-NPs had a clear，transparent and slightly bluish appearance. Under transmission electron microscopy，the nanoparticles appear spherical with an average particle size of（227.33±0.02）nm. The potential was（-23.6±0.02）mV，the encapsulation efficiency was（98.57±0.46）%，the drug loading was（8.57±0.001）%，and the polydispersity index（PDI）was（0.1369±0.26）%. MAG-WPI-NPs had DPPH radical scavenging effects，Escherichia coli inhibitory effects，and were more potent than free drugs.
This study prepared a natural food grade self-assembled MAG-WPI-NPs. The preparation method was simple，safe and non-toxic，significantly improving the poorwater solubility and low bioavailability of magnolol and laying the foundation for the clinical application of magnolol.
37
15
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
