Full
Outline
To observe the mechanism of electroacupuncture intervention on the activation of astrocytes in the hippocampal region of rats with cerebral ischemia-reperfusion injury and its effects on the expressions of reactive oxygen species（ROS），thioredoxin interacting protein（TXNIP）and nucleotidebinding oligomerization domain（NOD）-like receptor family pyrin domain containing 3（NLRP3）.
Forty-eight adult male SD rats were randomly divided into sham surgery group，model group，ROSinhibitor group and electroacupuncture group，with 12 rats in each group. The cerebral ischemia-reperfusion injury rat model was prepared using the traditional Koizumi suture method，and the rats were included or excluded according to the Longa scoring method. The sham surgery group only underwent the separation of the carotid artery. The inhibitor group received intragastric administration of the ROSinhibitor N-acetyl-L-cysteine solution for 10 days starting 3 days before modeling. The electroacupuncture group received intervention at Baihui（DU20），Yintang（DU29）and bilateral Zusanli（ST36）for 10 min per day for 7 days. Cylinder experiment was conducted to observe the recovery ofneurological function.TTC was used to detect the infarct area of the brain. The immunohistochemistry method was performed to detect the positive expression of GFAPin the hippocampal region.DHE fluorescence probe was used to detect ROScontent in the hippocampal region. Western blot was performed to detect the protein expressions of GFAP，TXNIP and NLRP3 in the hippocampal region，and immunofluorescence was used to determine the colocalization of TXNIPand NLRP3 in the hippocampal region.
Compared with the sham surgery group，the model group showed a significantly reduced asymmetry index，more severe neurological function damage（P<0.01），larger range of cerebral infarction area（P<0.01），increased expression and protein content of GFAP（P<0.01），less proliferation of astrocytes，weaker fiber protrusions of cell bodies，and significant increase in ROS，TXNIPand NLRP3（P<0.01）. Compared with the model group，the ROSinhibitor group and the electroacupuncture group showed higher asymmetry index，better recovery of neurological function（P<0.05，P<0.01），smaller range of cerebral infarction area（P<0.01），significantly increased expression and protein content of GFAP（P<0.01），increased activation of astrocyte proliferation，denser arrangement，increased degree of cell fiber protrusions，and decrease in the contents of ROS，TX-NIP and NLRP3（P<0.05，P<0.01），with the electroacupuncture group showing more significant effects than the inhibitor group.
Electroacupuncture intervention can restore neurological function damage in rats，which may be related to promoting the proliferation and activation of astrocytes，inhibiting the ROSTXNIP-NLRP3 signaling pathway，reducing oxidative stress and inflammatory reactions and exerting neuroprotective effects.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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