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Outline
Identification，content determination and characteristic spectrum analysis of different Chenxiang（Aquilariae Lignum Resinatum）components obtained by artificial induction method were carried out using thin layer chromatography（TLC）and high performance liquid chromatography（HPLC）to evaluate the quality and quantity of Chenxiang（Aquilariae Lignum Resinatum）.
This article used chloroform-ether（10：1）as the developing agent to perform TLC identification on this batch of Chenxiang（Aquilariae Lignum Resinatum）samples.Content determination and characteristic spectrum were performed using HPLC under the following chromatographic conditions.Waters Symmetry Shield TM RP18 column（4.6 mm×250 mm，5μm）was used.The acetonitrile 0.1% formic acid water was as the mobile phase.The column temperature was 30℃.The flow rate was 0.7 mL·min-1.The detection wavelength was 252 nm.The injection volume was 10μL.Finally，similarity evaluation，Principal Component Analysis（PCA）and Orthogonal Partial Least Squares Discriminant Analysis（OPLS-DA）were used to screen for chromatographic peaks that caused significant differences in different parts（trunk and branch）.
The results of TLC showed that the spots of the Chenxiang（Aquilariae Lignum Resinatum）samples were basically the same as those of the control herb.Examining the test product under the UV lamp at 254 nm，it was found that the color of the sample spots was more obvious in the branch than that in the main trunk when the Rf value was 0.38.The content determination results showed that the content of agarotetrol in Chenxiang（Aquilariae Lignum Resinatum）samples from different aroma forming parts ranged from 0.02% to 0.58%，and the average content of agarotetrol in the branches was higher than that in the stems.The characteristic spectrum results showed that there were 6 common characteristic peaks in the Chenxiang（Aquilariae Lignum Resinatum）sample，with a significant difference in the peak area of agarotetrol.Except for No.4，No.9，No.12 and No.13，the content of agarotetrol in the branches of the other groups was higher than that in the trunk.The similarity value of Chenxiang（Aquilariae Lignum Resinatum）trunk was between 0.842 and 0.999，and the similarity value of Chenxiang（Aquilariae Lignum Resinatum）branch was between 0.901 and 0.994，indicating a good branch similarity effect.The results of PCA and OPLS-DA analysis by chemometrics showed that peak 4 and peak 6 were the symbolic components of the quality difference of Chenxiang（Aquilariae Lignum Resinatum）aroma.
The present study comprehensively and effectively analyzed the chemical components of Chenxiang（Aquilariae Lignum Resinatum）samples obtained from the trunk and branch parts using TLC，content determination and characteristic chromatographic fingerprint analysis methods under the same artificial induction method.Apart from some samples showing significant individual differences，the Chenxiang（Aquilariae Lignum Resinatum）samples from the branch parts demonstrated more pronounced aroma effects，which provides a basis for future optimization of methods and in-depth research on aroma mechanisms.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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