Mass spectrometry imaging technology is a combination of mass spectrometry and imaging technology,which converts mass spectrometry signals into visualized images with spatial distribution characteristics through imaging software. In recent years,mass spectrometry imaging technology has been widely used for the analysis of plant tissue metabolites,providing new ideas and methods for the research of traditional Chinese medicinal plants. This article provided a review in three parts. Firstly,it reviewed matrix assisted laser ionization desorption mass spectrometry imaging(MADLI)technology,secondary ion mass spectrometry imaging(SIMS)technology,Desorption Electrospray Ionization Mass Spectrometry Imaging(DESI)technology and the principle of nanostructure induced mass spectrometry imaging technology were summarized. Secondly,the application examples of mass spectrometry imaging technology in the visualization analysis and identification of metabolites in traditional Chinese medicinal plants as well as the biosynthesis pathways of active ingredients were reviewed. Finally,the advantages and limitations of mass spectrometry imaging technology were summarized,and further prospects were made for the application of mass spectrometry imaging technology in traditional Chinese medicinal plants.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |