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The quantitative models of fructose，glucose，sucrose，raffinose and polysaccharide in Huaidihuang（Rehmannia glutinosa）were established based on mid-infrared spectroscopy technology.
The fructose，glucose，sucrose and raffinose in 50 batches of genuine Huaidihuang（Rehmannia glutinosa）were determined by high performance liquid chromatography（HPLC），the polysaccharide was determined by ultraviolet-visible spectroscopy（UV-Vis），and the infrared spectra of Huaidihuang（Rehmannia glutinosa）were collected by mid-infrared spectrometer. HPLCmethod，UV-Vis method and mid-infrared spectroscopy technology were combined to establish a determination model for the sugar component contents of in Huaidihuang（Rehmannia glutinosa）using TQ analysis software.
The model of principal component regression（PCR）＋mutiplicative signal correction（MSC）＋first derivative（1st Der）＋Savitzky-Golay fler（SG）was the best for fructose，the model of partial least squares（PLS）＋CONSTANT＋1st Der＋SG best for glucose，the model of stepwise multiple linear regression（SMLR）＋CONSTANT＋second derivative（2nd Der）＋SG best for sucrose，the model of SMLR＋standard normal variate（SNV）＋2nd Der＋Noris derivative fliler（ND）best for raffinose and the model of PLS＋SNV＋2nd Der＋ND best for polysaccharide. The correlation coefficients were 0.786 2，0.927 8，0.838 8，0.782 4 and 0.964 8，respectively，with an average absolute deviation of-0.05%，0.04%，0.07%，-0.02% and-0.02%，respectively. It is verified that the prediction effect of the model is good.
The quantitative model of sugar components in Huaidihuang（Rehmannia glutinosa）established by mid-infrared spectroscopy is stable and objective，which can provide a basis for rapid evaluation of sugar components in Huaidihuang（Rehmannia glutinosa）.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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