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Rapid and Nondestructive Identification of Dihuang（Rehmannia glutinosa）of Different Origins Based on Infrared Spectroscopy and High Performance Liquid Chromatography
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Lu QIAO1, 2, Ziyu YIN1, 3, Haohan DUAN1, Yingying XIE1, Keke XU1, 2, Yonghong LIU1, 2, Juxiang LI1, Chengming DONG1, 2, Lei WANG1
Chinese Archives of Traditional Chinese Medicine | 2025, 43(3) : 42 - 46
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Chinese Archives of Traditional Chinese Medicine | 2025, 43(3): 42-46
• Target on National Project •
Rapid and Nondestructive Identification of Dihuang（Rehmannia glutinosa）of Different Origins Based on Infrared Spectroscopy and High Performance Liquid Chromatography
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Lu QIAO1, 2, Ziyu YIN1, 3, Haohan DUAN1, Yingying XIE1, Keke XU1, 2, Yonghong LIU1, 2, Juxiang LI1, Chengming DONG1, 2, Lei WANG1
Affiliations
  • 1.School of Pharmacy，Henan University of Chinese Medicine，Zhengzhou 450046，Henan，China
  • 2.Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials，Zhengzhou 450046，Henan，China
  • 3.Beijing University of Chinese Medicine，Beijing 100105，China
Published: 2025-03-10 doi: 10.13193/j.issn.1673-7717.2025.03.007
Outline
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Objective

To carry out the rapid and nondestructive identification of Dihuang（Rehmannia glutinosa）of different origins.

Methods

The infrared absorption spectrum of a total of 166 batches of Dihuang（Rehmannia glutinosa）in 16 counties and districts in four provinces was collected for differential analysis，and the results of the analysis were verified by high performance liquid chromatography.

Results

The infrared spectra of the 166 samples were basically similar in peak shape，with the same absorption at 2928，1643，1419，1353，1238，1145，1053，925，799，700～543 cm-1. The differences between samples from the three sites in Henan Province were mainly at 1519～1494 cm-1. The differences between samples from different sites in Hebei Province were mainly at 1571 and 1020 cm-1. The differences between samples from different sites in Shanxi Province were mainly at 1790～1642，1440，1030 cm-1. Then among the differences between provinces，Shandong Province was significantly different from other provinces mainly at 1796，1718，1640，1606～1567，1492，1453，1277，1024 cm-1. Among of them，the main characteristic component in the band of 1640～1439 cm-1 was catalpol，and the strong absorption peak at 1052 cm-1 was C-O bond stretching vibration absorption in the structure of pistil side.

Conlusion

Infrared spectroscopy combined with HPLCcan provide ideas for the rapid and non-destructive identification of different origins of Dihuang（Rehmannia glutinosa）.

Dihuang（Rehmannia glutinosa）  /  mid-infrared spectroscopy  /  HPLC  /  different origins  /  clustering analysis
乔璐, 阴紫钰, 段浩瀚, 谢赢赢, 徐可可, 刘永红, 李菊香, 董诚明, 王磊. 基于中红外光谱技术结合HPLC对不同产地地黄快速无损鉴别. 中华中医药学刊, 2025 , 43 (3) : 42 -46 . DOI: 10.13193/j.issn.1673-7717.2025.03.007
Lu QIAO, Ziyu YIN, Haohan DUAN, Yingying XIE, Keke XU, Yonghong LIU, Juxiang LI, Chengming DONG, Lei WANG. Rapid and Nondestructive Identification of Dihuang（Rehmannia glutinosa）of Different Origins Based on Infrared Spectroscopy and High Performance Liquid Chromatography[J]. Chinese Archives of Traditional Chinese Medicine, 2025 , 43 (3) : 42 -46 . DOI: 10.13193/j.issn.1673-7717.2025.03.007
Appendix
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Year 2025 volume 43 Issue 3
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Article Info
doi: 10.13193/j.issn.1673-7717.2025.03.007
  • Online Date：2026-10-30
  • Published：2025-03-10
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Affiliations
    1.School of Pharmacy，Henan University of Chinese Medicine，Zhengzhou 450046，Henan，China
    2.Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials，Zhengzhou 450046，Henan，China
    3.Beijing University of Chinese Medicine，Beijing 100105，China
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https://castjournals.cast.org.cn/joweb/zhzyyxk/EN/10.13193/j.issn.1673-7717.2025.03.007
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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