Zuogui Pill（左归丸），from Jingyue Quanshu，has been used clinically for more than 400 years. It is a classic prescription for the treatment of kidney Yin deficiency. It has the effect of nourishing Yin and tonifying kidney，supplementing essence and replenishing marrow，and is widely used in the treatment of osteoporosis，knee osteoarthritis and other diseases. In recent years，more and more studies have shown its efficacy in the treatment of premature ovarian failure（POF）. This article summarized the mechanism of the treatment of POF and the literature in recent years from the aspects of inhibiting cell apoptosis and autophagy，improving sex hormone levels，regulating the expressions of growth differentiation factor 9（GDF-9）and bone morphogenetic protein-15（BMP-15），up-regulating the expressions of connexin37（Cx37）and Connexin43（Cx43），regulating the hypothalamic pituitary ovarian axis（HPOA），improving ovarian angiogenesis and anti osteoporosis. And it participated in regulating the PI3K/Akt/mTOR and GDF-9/Smad2 signaling pathways，confirming the therapeutic effect of Zuogui Pill on POF from a molecular mechanism perspective. And through the summary of Zuogui Pill，it is found that whether it is a single treatment，or combined with western medicine or acupuncture and moxibustion treatment，it can significantly improve the symptoms of patients，reduce adverse reactions，and has definite clinical efficacy and high safety，which is worthy of clinical promotion and application. This article comprehensively analyzed the current status of Zuogui Pill in the treatment of POF through basic and clinical research，and proposed shortcomings and improvements in current basic and clinical research，providing relevant basis for future in-depth research and clinical application of the formula，as well as the development of new drugs for the treatment of POF