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Outline
To investigate the regulatory effects of Dahuang Lingpi Formula（大黄灵脾方）on oxidative stress in rats with ischemia-reperfusion acute kidney injury（IRI）.
Male Sprague-Dawley（SD）rats were randomly divided into sham operation group，model group，Dahuang Lingpi Formula low and high dose groups with 16 rats in each group. The IRI model was established by clamping bilateral renal pedicles for 45 min and restoring blood flow reperfusion. The rats in Dahuang Lingpi Formula groups were given Dahuang Lingpi Formula by gavage，and the rats in the sham operation group and model group were given distilled water every day for continuous intervention for 7 days. On the 3rd and 7th day，8 rats in each group were randomly executed to collect samples to detect the contents of serum creatinine，urea，hepcidin，serum iron and renal iron，malondialdehyde（MDA），superoxide dismutase（SOD），glutathione peroxidase（GSH-Px）and interleukin-6（IL-6）. The renal histology was observed by HE staining. The expressions of hepatic hepcidin and renal ferroportin（FPN）were detected by immunohistochemistry and western blot（WB）. Real-time fluorescence quantitative reverse transcription polymerase chain reaction（RT-qPCR）was used to detect hepcidin mRNA expression in liver tissues.
Compared with those of the model group，the contents of serum creatinine，urea，serum iron，renal iron，MDA and IL-6 were significantly decreased（P<0.05）. The contents of serum hepcidin，SOD and GSH-Px were significantly increased（P<0.05）. HE staining showed that the pathological injury of kidney in Dahuang Lingpi Formula groups was significantly alleviated. RT-qPCR showed that the expression of hepcidin mRNA in Dahuang Lingpi Formula groups was significantly higher than that in the model group（P<0.05）. Immunohistochemical and WB results showed that the expressions of hepcidin in Dahuang Lingpi Formula groups were significantly higher than those in the mod-el group（P<0.05）. FPN protein expression was significantly lower than that of the model group（P<0.05）.
-Dahuang Lingpi Formula can alleviate oxidative stress and inflammation levels in IRIrats，promote renal injury repair and accelerate renal function recovery. The mechanism may be related to the intervention of oxidative stress related to iron metabolism abnormalities in IRI.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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