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Analysis of Chemical Components and Components Migrating to Blood of Guizhi Gancao Decoction（桂枝甘草汤）by UHPLC-Q-Exactive Orbitrap MS Method
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Ran YIN1, 2, Xinying YU3, Shanshan WEI2, Chongzhen SHI2, Shuang ZHAO3, Xue WANG3, Yuanfang SUN3, Fei HAN3
Chinese Archives of Traditional Chinese Medicine | 2025, 43(2) : 198 - 207
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Chinese Archives of Traditional Chinese Medicine | 2025, 43(2): 198-207
• Platform of Provincial Project •
Analysis of Chemical Components and Components Migrating to Blood of Guizhi Gancao Decoction（桂枝甘草汤）by UHPLC-Q-Exactive Orbitrap MS Method
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Ran YIN1, 2, Xinying YU3, Shanshan WEI2, Chongzhen SHI2, Shuang ZHAO3, Xue WANG3, Yuanfang SUN3, Fei HAN3
Affiliations
  • 1.Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications，Liaoning University of Traditional Chinese Medicine，Shenyang 110847，Liaoning，China
  • 2.College of Pharmacy，Liaoning University of Traditional Chinese Medicine，Dalian 116600，Liaoning，China
  • 3.School of Pharmacy，Shenyang Pharmaceutical University，Shenyang 110016，Liaoning，China
Published: 2025-02-10 doi: 10.13193/j.issn.1673-7717.2025.02.041
Outline
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Objective

To analyze and identify the chemical components of Guizhi Gancao Decoction（桂枝甘草汤）and its components migrating to blood after oral administration to rats using UHPLC-Q-Exactive Orbitrap MS method.

Methods

Chromatographic separation was carried out on XSelect HSST3 column（2.1 mm×150 mm，2.5μm）with gradient elution of acetonitrile-1% acetic acid solution as mobile phase at the flow rate of 0.3 mL/min. The column temperature was 35℃，and the detection was performed in positive and negative ion modes. The chemical components of Guizhi Gancao Decoction were analyzed according to the retention time，precise molecular weight，fragment ions，fragmentation pattern and comparison with the reference substances. By comparing the chromatographic and mass spectrometry information of blank plasma，medicated plasma and Guizhi Gancao Decoction samples，the components migrating to blood were analyzed.

Results

A total of 68 chemical components were identified in Guizhi Gancao Decoction，including 30 flavonoids，16 triterpenoids，8 organic acids，5 aldehydes，3 coumarins and 6 other components. After oral administration of Guizhi Gancao Decoction，a total of 45 prototype components were identified in rat plasma.

Conclusion

The established UHPLC-Q-Exactive Orbitrap MSmethod can rapidly characterize the whole chemical composition of Guizhi Gancao Decoction and its components migrating to blood in rats，providing a basis for further study on the pharmacodynamic material basis and quality control of Guizhi Gancao Decoction.

Guizhi Gancao Decoction  /  UHPLC-Q-Exactive Orbitrap MS  /  chemical components  /  components migrating to blood  /  identification
尹然, 于新颖, 魏姗姗, 石崇臻, 赵爽, 王雪, 孙媛方, 韩飞. 基于UHPLC-Q-Exactive Orbitrap MS技术分析桂枝甘草汤的化学成分及入血成分. 中华中医药学刊, 2025 , 43 (2) : 198 -207 . DOI: 10.13193/j.issn.1673-7717.2025.02.041
Ran YIN, Xinying YU, Shanshan WEI, Chongzhen SHI, Shuang ZHAO, Xue WANG, Yuanfang SUN, Fei HAN. Analysis of Chemical Components and Components Migrating to Blood of Guizhi Gancao Decoction（桂枝甘草汤）by UHPLC-Q-Exactive Orbitrap MS Method[J]. Chinese Archives of Traditional Chinese Medicine, 2025 , 43 (2) : 198 -207 . DOI: 10.13193/j.issn.1673-7717.2025.02.041
Appendix
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Year 2025 volume 43 Issue 2
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Article Info
doi: 10.13193/j.issn.1673-7717.2025.02.041
  • Online Date：2026-10-30
  • Published：2025-02-10
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Affiliations
    1.Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications，Liaoning University of Traditional Chinese Medicine，Shenyang 110847，Liaoning，China
    2.College of Pharmacy，Liaoning University of Traditional Chinese Medicine，Dalian 116600，Liaoning，China
    3.School of Pharmacy，Shenyang Pharmaceutical University，Shenyang 110016，Liaoning，China
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栓菌属 Trametes 5 2.39
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