Full
Outline
To evaluate the in vitro and in vivo antitumor activity of near-infrared light responsive elemene nanoemulsion thermosensitive hydrogel（ICG-ELE-NE-gel）on 4T1 breast cancer cells when combined with 808 nm near infrared irradiation.
The biological safety of ICG-ELE-NE-gel was evaluated by hemolysis test in vitro. The effect of ICGELE-NE-gel on the proliferation of 4T1 cells in vitro was determined by MTT assay. The uptake effect of 4T1 cells was qualitatively observed by using an inverted fluorescence microscopy. And the changes of cell migration ability were investigated by cell scratch test. Infrared thermal imaging test and in vivo pharmacodynamic study were performed after the orthotopic transplantation animal model of 4T1 breast cancer in BALB/c mice was established. At the end of the pharmacodynamic experiment，the levels of tumor necrosis factor-α（TNF-α），interleukin-6（IL-6）and interferon-γ（IFN-γ）in plasma of 4T1 tumor-bearing BALB/c mice were measured by ELISA. The pathological changes of the tumor tissues were observed by H&E and TUNEL staining.
ICG-ELE-NE-gel had high biological safety and good photothermal stability. ICG-ELE-NE-gel could inhibit the migration of 4T1 cells in a concentration-and time-dependent manner. ICG-ELE-NE-gel could be successfully taken up by 4T1 cells and significantly inhibited the horizontal migration of 4T1 cells. ICG-ELE-NE-gel and its laser group could significantly inhibit the growth of body weight and tumor volume of 4T1 tumor-bearing mice. The hydrogel could also effectively inhibit the expressions of TNF-αand IL-6，and promote the expression of IFN-γ. The H&E and TUNEL pathological sections showed that the tumor tissues in ICG-ELE-NE-gel group had obvious tumor cell apoptosis. When combined with 808 nm laser irradiation，there was more extensive cell damage or apoptosis.
ICG-ELE-NE-gel combined with near-infrared light irradiation could enhance the anti-tumor activity of 4T1 breast tumor in vitro and in vivo，and possibly realize the photothermal-chemical combination therapy of breast cancer.
31
15
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
