Full
Outline
To establish a gas chromatography mass spectrometry（GC-MS）technique using 1，1-Diphenyl-2-picrylhydrazyl（DPPH）as a free radical for rapid screening of antioxidant active components in the oil extracted from Bangmaoluoxinfu（Trichonephila clavata）.
The method combining GC-MSchromatographic analysis with DPPH radical chemical reaction was used to screen antioxidant components by analyzing the changes in the chromatographic of the oil extracted from Bangmaoluoxinfu（Trichonephila clavata）before and after reaction with DPPH. Furthermore，the antioxidant activity of each compound was evaluated by the rate of reduction of the peak area of the chromatograms. At the same time，to verify the screening results of antioxidants by the DPPH-GC-MSmethod，a methodological study was carried out，and the free radical scavenging ability of the control product was determined by a microplate reader.
A total of 22 compounds were identified from the oil extracted from Bangmaoluoxinfu（Trichonephila clavata），accounting for 89.67% of the total，mainly consisting of fatty acids and esters，with relatively high levels of methyl oleate and methyl linoleate. Sixteen antioxidants were rapidly screened from them by the DPPH-GC-MSmethod，among which 2，4-Di-tert-butylphenol，ethyl stearate，linoleic acid，ethyl linoleate，ethyl oleate and palmitic acid ethyl ester showed strong antioxidant capacity. The results of activity verification showed that the DPPH-GC-MS method was reliable and can be used for rapid screening and identification of antioxidants in natural products.
The DPPH-GC-MSmethod can simply and efficiently screen out the antioxidants in the oil extracted from Bangmaoluoxinfu（Trichonephila clavata），and provided the experimental basis for further development and utilization of Bangmaoluoxinfu（Trichonephila clavata）resources.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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