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Outline
In order to improve the physicochemical properties of isoliquiritigenin (ISO) and its anti-inflammatory activity, ISO-loaded γ-cyclodextrin metal-organic framework complex (γ-CD-MOF/ISO) was prepared.
①γ-CD-MOF was synthesized by hydrothermal synthesis method, and ISO was loaded to form γ-CD-MOF/ISO by agitating heater.γ-CD-MOF/ISO was characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectrometer (FR-IR). The drug loading of γ-CD-MOF/ISO was evaluated.②The anti-inflammatory activity of γ-CD-MOF/ISO was investigated in LPS-induced RAW 264.7 cell model in vitro. The effect of γ-CD-MOF/ISO on cell viability was detected by CCK-8 assay. NO content in the supernatant was detected by Griess assay and the levels of pro-inflammatory cytokines IL-6 and TNF-α were measured by ELISA kit. The expressions of COX-2 and iNOS in protein level were detected by Western blot (WB).
The morphology of γ-CD-MOF/ISO was cubic crystals with uniform size. Drug loading efficiency ofγ-CD-MOF/ISO was 5.01%±0.21%.γ-CD-MOF/ISO at 2.5 μg/mL had no toxin in RAW 264.7 cells.γ-CD-MOF/ISO (0.1, 0.5, 2.5 μg/mL) concentration-dependently inhibited the NO production, the secretion of pro-inflammatory cytokines IL-6 and TNF-α, and COX-2 and iNOS in LPS-induced RAW 264.7 cells.
γ-CD-MOF/ISO exhibits high biocompability and improves the anti-inflammatory activity, which provides a new formulation in the treatment of acute lung injury.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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