As a common kidney-tonifying Chinese medicine, Tusizi (Cuscutae Semen) is widely used in clinic, with reproductive protection, anti-oxidation, anti-tumor and other pharmacological effects, and Tusizi (Cuscutae Semen) is often combined with Gouqizi (Lycii Fructus), Danggui (Angelicae Sinensis Radix), Roucongrong (Cistanches Herba) and other drugs, in the form of drug pairs of prescription compatibility, synergistically playing a stronger clinical effect. This paper focused on the pharmacological action of Tusizi (Cuscutae Semen) and the clinical application and pharmacological action of Tusizi (Cuscutae Semen) commonly used drug pairs. Combined with modern network pharmacology research, the mechanism of action was discussed and summarized, and the clinical compatibility and mechanism of action of Tusizi (Cuscutae Semen) and its drug pairs were deeply discussed, so as to provide reference for further development and utilization of Tusizi (Cuscutae Semen) and its medicine pairs.