Full
Outline
To establish fingerprint and multi-components determination of substance benchmark of Shaoyao Gancao Decoction（芍药甘草汤，SGD）and provide reference for quality control and evaluation of SGD.
The separation was performed on Waters Symmetry® C18 column（250 mm×4.6 mm，5μm）with acetonitrile -0.1% formic acid for gradient elution. The HPLC fingerprint of 15 batches of substance benchmark were established，and the contents of active components in the substance benchmark of SGD were determined.
The HPLC fingerprint of 15 batches of substance benchmark were established and a total of 8 common peaks were identified. The similarity evaluation system of traditional Chinese medicine was calculated and the result showed the similarity of characteristic chromatogram was greater than 0.90. Combining cluster analysis，principal component analysis and partial least squares discriminant analysis，the 15 batches of samples were classified into two types（one for S1-S4，S11，S12，and one for S5-S10，S13-S15）. Six known components including gallic acid，albiflorin，paeoniflorin，liquiritin apioside，liquiritin and glycyrrhizic acid were determined. The results of content determination were 2.46-3.31 mg·g-1，8.71-12.45 mg·g-1，20.97-33.14 mg·g-1，3.94-6.09 mg·g-1，4.41-6.03 mg·g-1，7.45-11.21 mg·g-1，respectively.
The HPLC fingerprint combined with multi-components determination is stable and reliable. This study can lay a foundation for the quality control and evaluation of SGD.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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