To investigate the effects of different processing methods on the active parts and characteristic components of Maidong(Ophiopogonis Radix),and to identify the main components that affect the changes of medicinal properties.
The samples of different regions of Maidong(Ophiopogonis Radix)fresh herbs,slices and processed products were prepared. The total saponins,total flavonoids and total polysaccharides were used as the effective parts by Ultraviolet-visible spectroscopy(UV-Vis)method. Then,methyl ophiopogon flavanone A,methyl ophiopogon flavanone B,Ophiopogon saponins C,Ophiopogon saponins D and Ophiopogon saponins D'were used as the characteristic components by High Performance Liquid Chromatography-Evaporative Light Scattering Detection(HPLC-ELSD). The two methods were combined for the multivariate statistical analysis to explore the key factors affecting the quality and drug properties of the processed products.
Although different batches of processed products came from different origins,their content changes were relatively consistent after different processing methods.
It was preliminarily determined that the decrease of cold property after processing of Maidong(Ophiopogonis Radix)was related to the changes in the contents and types of flavonoids and saponins.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |