Full
Outline
To analyze the volatile organic compounds（VOCs）in the soils associated with wild Dihuang（Rehmannia glutinosa）from different regions，providing a theoretical basis for exploring the causes of differences in Dihuang（Rehmannia glutinosa）medicinal materials across these regions.
A total of 35 batches of wild Dihuang（Rehmannia glutinosa）-associated soils from the authentic and the non-authentic production areas were studied. The VOCs in the dichloromethane and ethyl acetate extracts were analyzed using gas chromatography-mass spectrometry（GC-MS）. Multivariate statistical analysis was performed on the normalized data using software such as SIMCA 14.1 and SPSS 24 to identify differences in VOCs between the authentic and the non-authentic regions.
The VOCs in the dichloromethane extracts primarily included esters，alcohols，alkanes and phenols，with significant differences in the relative contents of alkanes，phenols and esters between the authentic and the non-authentic regions. The ethyl acetate extracts mainly contained esters，alkanes，alcohols，phenols，amides and alkenes，with significant differences in these components between the regions. Multivariate statistical analysis indicated that soil samples were distinctly grouped by their region，with alkanes，acids and esters being the main contributors to the observed differences.
Although the types of VOCs in soils associated with wild Dihuang（Rehmannia glutinosa）are similar between the authentic and the non-authentic regions，their relative contents differ significantly，with alkanes，acids and esters being the primary differentiating compounds.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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