Full
Outline
To investigate the effect of Tectoridin（Tec）on autophagy and apoptosis of human gastric cancer cell line HGC-27.
The cell proliferation ability was detected by CCK-8 and clone formation assay，the cell apoptosis rate was detected by flow cytometry，and the autophagy inhibitor 3-MA was used to detect the autophagy. Western blotting was used to detect the protein expression levels of Beclin-1，Bcl-2，Bax and p53. Bioinformatics methods were used to predict the potential targets and signaling pathways，molecular docking was used to evaluate the binding degree between targets and Tec. Western blotting was used to detect the expressions of PI3K/Akt/mTOR pathway related proteins.
Tec can inhibit gastric cancer proliferation in a time-dependent and dose-dependent manner，promote the autophagy and apoptosis of HGC-27 cells，up-regulate the protein expressions of autophagy factor Beclin-1，apoptosis factor p53 and Bax，and reduce the protein expressions of apoptosis molecule Bcl-2. PPI network visualization analysis showed a strong affinity between Tec and targets such as AKT1，while KEGG enrichment analysis suggested that the inhibitory effect of Tec on gastric cancer may be closely related to the PI3K/Akt/mTOR signaling pathway. The protein expressions of the PI3K/Akt/mTOR signaling pathway，including p-PI3K，p-Akt and p-mTOR，were down-regulated.
Tec can induce the cell apoptosis and autophagy through the PI3K/Akt/mTOR pathway to inhibit the growth of HGC-27 cells.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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