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Outline
To investigate the intervention effect and mechanism of Jiawei Wendan Decoction（加味温胆汤）on insulin resistance（IR）in obesity type 2 diabetes mellitus（T2DM）rats based on PI3K/AKT-GLUT4 signaling pathway.
Using high-fat feeding induced spontaneous diabetic obese rats（Zucker diabetic fatty, ZDF）, 5 SPF-grade 5 weeks old lean control（fa/+）males were the blank controls, and 10 ZDF（fa/fa）male rats were randomly divided into model control group and Jiawei Wendan Decoction group. The concentration of Jiawei Wendan Decoction was 9.04 g/（kg·d）, while the blank control group and the model control group were given the same volume ultrapure water daily by gavage for 6 weeks. Body weights, food intakes and the levels of fasting blood glucose（FBG）were measured regularly every week, and oral glucose tolerance test（OGTT）was performed at the end of the experiment. The mRNA and protein expression levels of insulin receptor-β（INSR-β）, phosphoinositol 3-kinase（PI3K）, protein kinase B（AKT）, phosphorylated-protein kinase B（p-AKT）and glucose transporter 4（GLUT4）were detected in skeletal muscle tissue by real-time fluorescence quantitative PCR experiment（RT-qPCR）and Western blotting experiment（WB）.
Compared with those of the blank control group, the rats of the model control group showed a significant increase in body weight in the early stage, and showed a significant decrease in body weight during the hyperglycemia stage. However, the food intake and FBG increased significantly, and the glucose tolerance decreased significantly. Compared with the model control group, the Jiawei Wendan Decoction group was able to significantly control weekly weight gain and food intake, significantly reducedthe FBG levels, and significantly increased the glucose tolerance. Compared with those in the blank control group, the mRNA and protein expression levels of INSR（β）, PI3K-p85, AKT, p-AKT and GLUT4 were significantly reduced in the model control group. Compared with those in the model control group, the mRNA and protein expression levels of INSR（β）, PI3K-p85, AKT, p-AKT and GLUT4 were significantly increased in the Jiawei Wendan Decoction group.
Jiawei Wendan Decoction can effectively reduce the blood glucose and improve the glucose tolerance. The mechanism may be related to the regulation of IR by the PI3K/AKT-GLUT4 pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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