To establish the infrared fingerprint spectra of standard decoctions of Xiangfu(Cyperi Rhizoma)and its processed products,and quickly identify the standard decoctions of Xiangfu(Cyperi Rhizoma)and its processed products.
Infrared fingerprint spectra were established for the decoctions of Xiangfu(Cyperi Rhizoma),wine-processed Xiangfu(Cyperi Rhizoma),vinegar-processed Xiangfu(Cyperi Rhizoma)and four-processed Xiangfu(Cyperi Rhizoma).The differences among the average infrared spectra of the decoctions of Xiangfu(Cyperi Rhizoma),wine-processed Xiangfu(Cyperi Rhizoma),vinegar-processed Xiangfu(Cyperi Rhizoma)and four-processed Xiangfu(Cyperi Rhizoma)were analyzed using OMNIC 9.2 software.The common peaks of Xiangfu(Cyperi Rhizoma)and its processed product decoctions were identified using Spectrum forWindow 3.02 software and the relative peak heights were calculated.The differences among the processed Xiangfu(Cyperi Rhizoma)decoctions were compared using principal component analysis,orthogonal partial least squares-discriminant analysis and clustering analysis.
A total of 12 common peaks were identified and standardized for the decoctions of Xiangfu(Cyperi Rhizoma),vinegar-processed Xiangfu(Cyperi Rhizoma),wine-processed Xiangfu(Cyperi Rhizoma)and four-processed Xiangfu(Cyperi Rhizoma)using baseline correction and normalization.The differences were obvious in the 1700-1000 cm-1 waveband,and fourmain contribution wavebands were selected,including2929 cm-1,1053 cm-1,997 cm-1 and 1639 cm-1.The processed Xiangfu(Cyperi Rhizoma)decoctionswere all clustered into four categories through analysis.
There are obvious differences among the decoctions of Xiangfu(Cyperi Rhizoma),vinegar-processed Xiangfu(Cyperi Rhizoma),wine-processed Xiangfu(Cyperi Rhizoma)and four-processed Xiangfu(Cyperi Rhizoma),and they can be quickly identified using infrared spectroscopy.The method is fast,effective and accurate.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |