To study the effects of Yifei JianpiRecipe（益肺健脾方）on aquaporin 3（AQP3），aquaporin 5（AQP5）and mucin 5AC（MUC5AC）in bronchial and lung tissues of chronic obstructive pulmonary disease（COPD）model ratswith lung and spleen deficiency syndrome.

Sprague-dawley rats were randomly segmented into blank group，model group，ambroxol group，dexamethasone group and Yifei Jianpi Recipe high，medium and low dose groups.The COPD ratmodel of lung and spleen deficiency was created by fumigation combined with lipopolysaccharide tracheal drip and Senna gavage and the pathologic morphology of bronchopulmonary tissues in rats was observed by hematoxylin-eosin（HE）staining and Alcian Blue-Periodic Acid Schiff（AB-PAS）staining，and the m RNA expressions of AQP3，AQP5 and MUC5AC in ratbronchopulmonary tissueswere detected by RT-qPCR，and protein expressions of AQP3，AQP5 and MUC5ACwere detected by Western Blot and immunohisto chemistry（IHC）methods，the mRNA expressions of AQP3，AQP5 and MUC5AC in rat bronchopulmonary tissues were detected by quantitative real-time polymerase chain reaction（RT-qPCR），and the protein expressions of AQP3，AQP5 and MUC5AC in rat bronchopulmonary tissues were detected by Western Blotand IHC methods.

Compared with those in the sham group，the bronchopulmonary tissues of rats in themodel group showed different degrees of damage，and HE staining demonstrated that there were tangible inflammatory cell infiltration in the airways of rats，and AB-PASstaining reviewed thata good deal of cupshaped cell hyperplasia could be seen in the bronchial tubes of rats.ThemRNA and protein expressions of AQP3 and AQP5 were significantly decreased and MUC5AC mRNA and protein expressions were clearly increased in the model group（P<0.01）.Compared with those in the modelgroup，the inflammatory pathology damage of the bronchopulmonary tissues and the proliferation of cup cells in the rats of each dose group showed different degrees of improvement，and the m RNA and protein expressions of AQP3 and AQP5 were increased to different degrees.Among them，the Yifei Jianpi Recipe high dose group，the aminoglossum group and the dexamethasone group showed significant and comparable levels of increase（P<0.05）；the MUC5AC mRNA and protein expressions were reduced to different degrees，among which the ambroxol group，the dexamethasone group and the Yifei Jianpi Recipe high dose group showed significant and comparable reductions（P<0.01）.

Yifei Jianpi Recipe can inhibit airway mucus hypersecretion and reduce airway inflammation in COPD model rats with lung and spleen deficiency syndrome，and its mechanism is related to up-regulation of AQP3 and AQP5 and down-regulation of MUC5AC transcription and expression.