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This study aimed to screen dominant lactic acid bacteria (LAB) for Gegen (Pueraria lobata) fermentation and optimize the synergistic fermentation processes to enhance the bioactive compound yields.
By monitoring the dynamic changes in bacterial activity, total acid, total flavonoids and antioxidant activity during the fermentation process of six strains of LAB, Analytic Hierarchy Process (AHP)-Entropy Weight Method was used to comprehensively evaluate and screen the dominant LAB and optimize the key process parameters such as strain ratio, inoculation amount and solid-liquid ratio based on the synergistic fermentation strategy of dominant bacteria.
Lactobacillus plantarum P9 and Lactobacillus rhamnosus were the dominant strains for Gegen (Pueraria lobata) fermentation. The optimal process conditions for their synergistic fermentation were a strain ratio of 3∶1, inoculation volume of 10%, solid-liquid ratio of 1∶25, temperature of 37 ℃ and fermentation for 3 d. Under these conditions, the viable counts reached 8.96 lgCFU·mL-1 (231.6% higher than Lactobacillus plantarum P 9 monoculture, 31.83% higher than Lactobacillus rhamnosus), total acid content peaked at 2.95 g·L-1 (91.6% increase over Lactobacillus rhamnosus monoculture), and daidzein content achieved 1.32 mg·g-1 (164% and 238% enhancements compared to respective monocultures).
Microbial consortia integration synergistically amplified strain-specific advantages, significantly elevating bioactive metabolites and providing strain selection and process optimization strategy for the development of fermented functional beverages and probiotic preparations based on Gegen (Pueraria lobata).
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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