Full
Outline
To analyze the quality of different forms of Dihuang（Radix et Rhizoma Dioscorea）（juice decoction, fresh dried decoction, decoction）, to determine the contents of ingredients and establish the fingerprint map of different forms of Dihuang（Radix et Rhizoma Dioscorea）, so as to provide a basis for the clinical application of different forms.
High Performance Liquid Chromatography（HPLC）measured the contents of three glycosides and five glycosides of Dihuang（Radix et Rhizoma Dioscorea）and established the HPLC fingerprint of different treatment forms.
The content order of three kinds of cyclic terpenoid components was fresh dried Dihuang（Radix et Rhizoma Dioscorea）decoction > Dihuang（Radix et Rhizoma Dioscorea） juice decoction > Dihuang（Radix et Rhizoma Dioscorea）decoction；the content order of 5 kinds of sugar was Dihuang（Radix et Rhizoma Dioscorea）juice decoction > fresh dried Dihuang（Radix et Rhizoma Dioscorea）decoction > Dihuang（Radix et Rhizoma Dioscorea）decoction. Twelve common peaks were calibrated in the fingerprint spectrum of different processing forms of Dihuang（Radix et Rhizoma Dioscorea）. Through the control comparison, six total peaks were identified, which were peach leaf coridin, reflavin D, reflavin A, motherthidin and hair flower glycoside. The similarity results showed that the similarity of different treatment forms of 30 batches ranged from 0.835 to 0.994, indicating a good similarity of the established fingerprint. Principal component analysis（PCA）and orthogonal least squares discriminant analysis（OPLS-DA）analysis showed differences in the quality of different treatment forms, and the main landmark components that differentiated different treatment forms were peaks 1, 2, 11, 12, 3（catalpol）, 10 and 5.
In this study, the contents of different forms of Dihuang（Radix et Rhizoma Dioscorea）（decoction, fresh dried decoction and decoction）were determined, and the fingerprint of different forms of Dihuang（Radix et Rhizoma Dioscorea）was established, which provided the research basis and theoretical basis for the primary processing of fresh Dihuang（Radix et Rhizoma Dioscorea）and the clinical use of the juice.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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