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Protective Mechanism of Cardiomyocytes by Yiqi Wenyang Huoxue Lishui Recipe（益气温阳活血利水方）Through Regulating MLK3-Mediated Mitochondrial Unfolded Protein Response
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Zhennan LI1, 2, Zhongzheng ZHANG2, Kang XIE3, Haowen ZHUANG3, Junyan WANG2, 3
Chinese Archives of Traditional Chinese Medicine | 2026, 44(4) : 196 - 200
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Chinese Archives of Traditional Chinese Medicine | 2026, 44(4): 196-200
• Target on National Project •
Protective Mechanism of Cardiomyocytes by Yiqi Wenyang Huoxue Lishui Recipe（益气温阳活血利水方）Through Regulating MLK3-Mediated Mitochondrial Unfolded Protein Response
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Zhennan LI1, 2, Zhongzheng ZHANG2, Kang XIE3, Haowen ZHUANG3, Junyan WANG2, 3
Affiliations
  • 1.College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China
  • 2.College of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, China
  • 3.The First Clinical Medical School of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong, China
Published: 2026-04-10 doi: 10.13193/j.issn.1673-7717.2026.04.038
Outline
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Objective

The study aimed to observe the cardioprotective effects of Yiqi Wenyang Huoxue Lishui Recipe（益气温阳活血利水方, YWHLR）by regulating the mixed lineage kinase 3（MLK3）mediated mitochondrial unfolded protein response（UPRmt）and to explore the mechanism of YWHLR in preventing and treating heart failure.

Method

The mouse HL-1 cardiomyocyte model was prepared using angiotensin Ⅱ（AngⅡ）, followed by interventions with URMC-099, Oligomycin A and YWHLR medicated serum. The mice were divided into control, AngⅡ, AngⅡ + URMC-099, AngⅡ + Oligomycin and AngⅡ + YWHLR groups. Cell viability and proliferation were assessed using the CCK-8 assay. RT-PCR measured the levels of MLK3 and UPRmt mRNA（MLK3, CHOP, LONP1, HSP60, HSP70）, and Western blot analyzed the protein levels related to MLK3 and UPRmt（MLK3, CHOP, LONP1, HSP60, HSP70）. Immunofluorescence double staining was employed to detect the expressions of HSP70 and MLK3 proteins.

Results

Compared to the control group, the AngⅡ group showed a significant increase in MLK3 expression（P<0.01）and a significant decrease in the levels of CHOP, LONP1, HSP60 and HSP70（P<0.01）. The AngⅡ + URMC-099 group exhibited a significant decrease in MLK3 expression（P<0.01）and a significant increase in the levels of CHOP, LONP1, HSP60 and HSP70（P<0.01）compared to the AngⅡ group. The AngⅡ + Oligomycin group showed no significant difference in MLK3 expression（P>0.05）but a significant increase in the levels of CHOP, LONP1, HSP60 and HSP70（P<0.01）. The AngⅡ + YWHLR group demonstrated a significant decrease in MLK3 expression（P<0.01）and a significant increase in the levels of CHOP, LONP1, HSP60 and HSP70（P<0.01）compared to the AngⅡ group.

Conclusion

YWHLR medicated serum may exert its therapeutic effects by modulating the MLK3-mediated mitochondrial unfolded protein response.

mitochondrial unfolded protein response  /  Yiqi Wenyang Huoxue Lishui Recipe（益气温阳活血利水方）  /  heart failure  /  MLK3
李镇南, 张中正, 谢抗, 庄皓文, 王俊岩. 益气温阳活血利水方调控MLK3介导的线粒体未折叠蛋白反应保护心肌细胞作用机制研究. 中华中医药学刊, 2026 , 44 (4) : 196 -200 . DOI: 10.13193/j.issn.1673-7717.2026.04.038
Zhennan LI, Zhongzheng ZHANG, Kang XIE, Haowen ZHUANG, Junyan WANG. Protective Mechanism of Cardiomyocytes by Yiqi Wenyang Huoxue Lishui Recipe（益气温阳活血利水方）Through Regulating MLK3-Mediated Mitochondrial Unfolded Protein Response[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (4) : 196 -200 . DOI: 10.13193/j.issn.1673-7717.2026.04.038
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Year 2026 volume 44 Issue 4
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Article Info
doi: 10.13193/j.issn.1673-7717.2026.04.038
  • Online Date：2026-10-14
  • Published：2026-04-10
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Affiliations
    1.College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China
    2.College of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, China
    3.The First Clinical Medical School of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
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Genus		 种数
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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