By analyzing the Fourier Transform Infrared Spectroscopy(FT-IR)spectrum of Xiangfu(Cyperi Rhizoma) from different origins, the main contribution band of the differences among the samples from different regions can be founded, so as to trace the origin of Xiangfu(Cyperi Rhizoma).
Using FT-IR and combining a variety of chemometric methods, 30 batches of samples from 3 producing areas were analyzed. Then the characteristic band peaks were screened according to the main active components, getting the main difference bands of the samples.
Selecting 991, 1018, 526, 1081, 2930, 932, 1514, 708, 579, 1159, 763, 1461 cm-1 as the main difference contribution bands, and the results of Hierarchical Cluster Analysis(HCA), Principal Component Analysis(PCA)and Orthogonal Partial Least Squares Discriminant Analysis(OPLS-DA)could be clustered into three categories.
According to the selected characteristic bands, Xiangfu(Cyperi Rhizoma) from different producing areas can be effectively distinguished. The experimental results also show that the differences of saccharides and acids in Xiangfu(Cyperi Rhizoma)from different producing areas can be focused on.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |