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Outline
To reveal whether Formononetin（FMN）inhibits ferroptosis in HT22 cells induced by oxygen glucose deprivation/reoxygenation by activating the nuclear factor erythroid 2-related factor 2（Nrf2）/Glutathione peroxidase 4（GPX4）？pathway.
HT22 cells were subjected to oxygen-glucose deprivation/reoxygenation（OGD/R）damage to create an in vitro model, and HT22 cells were divided into control group（CON）, FMN group（FMN）, model group（OGD/R）, model + FMN group（OGD/R + FMN）, model + ML385 group（OGD/R + ML385）, model + RSL3 group（OGD/R + RSL3）. The CCK-8 method was used to detect the cell viability. DCFH-DA probe was used to detect the level of intracellular ROS. TUNEL staining and flow cytometry were used to detect the apoptosis. The colorimetric method was used to detect Fe2+ content in cells. Western blot was used to detect the protein expression levels of Nrf2 and GPX4.
Compared with those in the CON group, the cell proliferation activity in the OGD/R group was significantly reduced, the Fe2+ content, ROS level and apoptosis ratio were significantly increased, and the protein expressions of Nrf2 and GPX4 were significantly decreased（P > 0.05）. Compared with the OGD/R group, the OGD/R + FMN group had significantly increased cell proliferation activity（P < 0.05）, Fe2+ content, ROS level and apoptosis ratio significantly decreased and the expressions of Nrf2 and GPX4 proteins increased significantly. Compared with those of the OGD/R group, the cell proliferative activity of the OGD/R + ML385 group and the OGD/R + RSL3 group was significantly decreased（P < 0.05）, the Fe2+ content, ROS level and apoptosis ratio were significantly increased, the expression of Nrf2 protein in the OGD/R + ML835 group was significantly decreased（P < 0.05）, and the expression of GPX4 protein in the OGD/R + RSL3 group was significantly decreased（P < 0.05）.
FMN may inhibit ferroptosis induced by oxygen glucose deprivation/reoxygenation by activating the Nrf2/GPX4 pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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