Full
Outline
This study observed the effects of Jianpi Huatan Formula（健脾化痰方, JPHTF）on oxidized low-density lipoprotein（ox-LDL）induced vascular smooth muscle cell viability, proliferation, phenotype transformation and inflammatory factors in vitro.
Smooth muscle cells were isolated from the thoracic aorta of C57BL/6J mice. The 6-8 week old Sprague Dawley rats were orally administered, and the blood samples were collected from the abdominal aorta to prepare the drug containing serum. The DMEM medium culture was the control group. The ox-LDL induction（100 μg/mL）was the ox-LDL group. The atorvastatin group were the serum containing ox-LDL and atorvastatin（0.4 μM）. JPHTF low, middle and high dose groups were the serum containing ox-LDL and JPHTF（5%, 10%, 15%）. The identification of the first generation vascular smooth muscle cells？（VSMCs）was carried out by using immunofluorescence staining with α-smooth muscle actin（α-SMA）antibody. The cell counting kit-8（CCK-8）method was used to detect the cell viability. Transwell assay was used to observe the cell migration. Western Blot was used to detect the expressions of α-SMA, Calponin, SM22α, proliferating cell nuclear antigen（PCNA）, osteopontin（OPN）, ASC, NOD-like receptor family pyrin domain containing 3（NLRP3）and cleaved caspase-1 in cells, and enzyme-linked immunosorbent assay（ELISA）was used to detect the levels of interleukin-1β（IL-1β）, interleukin -18（IL-18）, tumor necrosis factor-α（TNF-α）and interleukin-6（IL-6）in cell supernatant.
JPHTF inhibited ox-LDL induced VSMCs proliferation and phenotype transition. JPHTF reduced the protein expression levels of NLRP3, ASC and cleaved caspase-1. ELISA results showed that JPHTF significantly reduced the levels of IL-1β and IL-18, as well as the levels of TNF-α and IL-6.
The effect of JPHTF in vitro can inhibit the conversion of VSMCs induced by ox-LDL from contractile phenotype to synthetic phenotype, reduce the ability of cell proliferation and migration, lower the levels of NL-RP3, ASC and cleaved caspase-1, and inhibit the activation of inflammasome, thereby inhibiting the development of atherosclerosis.
91
17
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
