Full
Outline
To investigate the ameliorative effects of dredging Du meridian and regulating spirit acupuncture on cognitive impairment in stroke rats and its potential molecular mechanisms in regulating mitochondrial ferroptosis.
Seventy rats were randomly divided into a normal control group（19 rats），a sham operation group（19 rats），a stroke model group（16 rats）and dredging Du meridian and regulating spirit acupuncture group（16 rats）. The normal control group did not receive any intervention，while the stroke model group used thread embolization to prepare a stroke rat model. The sham operation group only exposed the neck arteries，but did not receive the thread embolization treatment. The rats in the dredging Du meridian and regulating spirit acupuncture group were subjected to acupuncture at“Baihui（DU20）”and“Shenting（DU24）”，with the addition of an electroacupuncture device. The other group were simply fixed in the same environment without any other intervention measures. The stroke model was established in rats by filament occlusion method. Morris water maze test was performed to evaluate the learning and memory abilities. Brain infarct area was detected by TTC staining. The levels of Fe2+ and glutathione（GSH）in brain tissues were measured biochemically. The expressions of transferrin receptor（TfR1），iron regulatory protein 1（IRP1）and ferroportin（FPN）related to mitochondrial iron metabolism were analyzed by real-time PCR and Western blot.
Compared with the normal controls，the stroke model rats exhibited significant cognitive impairment，increased brain infarct area，elevated Fe2+ level and reduced GSH level in brain tissues，up-regulated TfR1 expression and down-regulated expressions of IRP1 and FPN. Dredging Du meridian and regulating spirit acupuncture ameliorated these alterations，suggesting its role in maintaining cellular iron homeostasis and inhibiting ferroptosis.
Dredging Du meridian and regulating spirit acupuncture significantly improved cognitive dysfunction and brain infarction in stroke rats，likely by regulating the expressions of iron metabolism-related molecules to restore cellular iron homeostasis，thereby attenuating neuronal damage induced by mitochondrial ferroptosis.
104
16
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
