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Outline
Based on ultra performance liquid chromatography-orbitrap fusion lumos tribrid-mass spectrometry（UPLC-Orbitrap Fusion Lumos Tribrid-MS），the migration components in serum of rats after intragastric administration of water extract from Nyuzhenzi（Ligustri Lucidi Fructus，LLF）and wine steamed Nyuzhenzi（Ligustri Lucidi Fructus）（WLLF）were compared and analyzed.
Male SD rats were randomly divided into blank group，LLF group（10.8 g·kg-1·d-1）and WLLF group（10.8 g·kg-1·d-1），with 6 rats in each group. The administration groups were given the water extract of LLF and WLLF，respectively，and the blank group was given the equal volume of purified water，once in the morning and once in the evening，for 5 consecutive days. One hour after the last administration，the blood was taken from the abdominal aorta to prepare the serum samples. AccucoreTM C18（100mm×2.1mm，2.6μm）chromatographic column was used. The mobile phase was acetonitrile（A）-0.1% formic acid water（B），with the gradient elution（0-5 min，95% B→85% B；5-10 min，85% B→73% B；10-24 min，73% B→15% B）. The flow rate was 0.2 mL·min-1. The injection volume was 5 μL. The positive and negative ion mode scanning was used，with the scanning range of m/z 120-1200. Compound Discoverer 3.3 software was used to analyze and identify the prototype components and metabolites of LLF and WLLF into blood according to mass spectrometry data and related literature. The multivariate statistical analysis method was used to compare the different components between the medicated serum of LLF and WLLF.
A total of 64 blood components were identified in the serum of rats given the water extract of LLF，including 40 prototype components and 24 metabolites. A total of 57 blood components were identified in the serum of rats given the water extract of WLLF including 35 prototype components and 22 metabolites. The prototype components mainly included phenylethanoid glycosides，iridoids，triterpenoids，flavonoids，etc. The metabolic pathways mainly included hydroxylation，methylation，glucuronidation，etc. According to the variable importance in projection（VIP）value>1，student's t test results were not significant（P<0.05），12 differential blood components such as specnuezhenide and ligustrosidic acid were screened，including 7 prototype components and 5 metabolites.
The serum migration components of LLF changed significantly after wine steaming，which could provide a theoretical basis for elucidating the material basis of LLF and WLLF.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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