Full
Outline
To investigate the effect of Huangqin Decoction（黄芩汤，HQD）on ferroptosis in ulcerative colitis based on kelch-like ECH-associated protein 1（Keap1）/nuclear factor-erythroid 2 related factor 2（Nrf2）pathway.
The mice in the in vivo experiment were divided into normal group，model group，traditional Chinese medicine group（HQD group）and mesalazine group. The ulcerative colitis mice were induced by dextran sulfate sodium. HE staining was used to observe the histo-pathology of colon. The contents of Fe in tissues were detected. The mRNA expression levels of glutathione peroxidase 4（GPX4），prostaglandin-endoperoxide synthase 2（PTGS2），Nrf2，Keap1 and heme oxygenase-1（HO-1）were detected by quantitative polymerase chain reaction（q-PCR）. The cells in the vitro experiments were divided into normal group，model group，HQD group and inhibitor group（ML385），and the cell ferroptosis model was constructed with Erastin. The cell growth was detected by CCK-8. The contents of Fe and lactate dehydrogenase（LDH）were detected. The mRNA expression levels of GPX4，PTGS2，Nrf2，Keap1 and HO-1 were detected by q-PCR. The protein expression levels of GPX4，PTGS2，Nrf2，Keap1 and HO-1 were detected by Western Blot.
In the in vivo experiments，compared with the model group，the HQD group and the mesalazine group obtained better results in ameliorating colon injury. In comparison to the model group，the Fe content was significantly decreased in the HQD group and the mesalazine group. In comparison to model group，the mRNA expression levels of PTGS2，HO-1，Nrf2 and Keap1 were decreased，the mRNA expression level of GPX4 was increased in both the HQD group and the mesalazine group. Compared with that of the model group，the cell vitality of the HQD group and the ML385 group were significantly increased. In comparison to the model group，the contents of Fe and LDH in the HQD group and the ML385 group were observably decreased. In comparison to that in the model group，the mRNA expression levels of PTGS2，HO-1，Nrf2 and Keap1 in the HQD group and the ML385 group were significantly decreased，while the level of GPX4 was increased visibly. In comparison to those in the model group，the protein expressions of PTGS2，Nrf2，Keap1 and HO-1 in the HQD group and the ML385 group were decreased，while the level of GPX4 was increased.
The mechanism of HQD inhibiting ferroptosis in ulcerative colitis may be the regulation of Keap1/Nrf2 pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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