To compare the effects of fresh cut pieces and traditional pieces of Huangqin(Scutellariae Radix),providing scientific basis for quality control of Huangqin(Scutellariae Radix)pieces with different processing method.
Ultraviolet spectrophotometry(UV)and ultra-high performance liquid chromatography(UPLC)and other methods were used to determine the contents of 6 index components(baicalin,wogonoside,baicalein,wogonin,total flavonoids,alcohol-soluble extract)in 24 batches fresh cut and traditional pieces of Huangqin(Scutellariae Radix)from different producing areas as samples. The bivariate correlation analysis of Huangqin(Scutellariae Radix)pieces was conducted by SPSS22.0 software in terms of the content of alcohol-soluble extract,total flavonoids,baicalin,wogonoside,baicalein and wogonin.
The content of alcohol-soluble extract in 24 batches of Huangqin(Scutellariae Radix)fresh cut pieces were(46.44±0.06)%~(55.26±0.21)%,the contents of total flavonoids,baicalin,wogonoside,baicalein and wogonin were(215.90±0.14)~(288.87±0.21)mg/g,(150.10±0.34)~(199.40±0.19)mg/g,(27.72±0.04)~(39.85±0.15)mg/g,(2.45±0.00)~(8.63±0.01)mg/g,(0.56±0.00)~(2.43±0.01)mg/g,respectively. The contents of alcohol-soluble extract in 24 batches of Huangqin(Scutellariae Radix)traditional pieces were(44.69±0.04)%~(54.24±0.07)%,the contents of total flavonoids,baicalin,wogonoside,baicalein and wogonin were(214.16±0.14)~(286.08±0.00)mg/g,(155.82±0.08)~(197.62±0.18)mg/g,(28.16±0.02)~(38.24±0.00)mg/g,(2.11±0.02)~(5.84±0.03)mg/g,(0.43±0.00)~(1.24±0.00)mg/g,respectively. Compared with those in the traditional cutting pieces,the contents of baicalein and wogonin were higher in fresh cutting pieces,whereas that of baicalin was lower in the fresh cutting pieces. Comprehensive evaluation of Huangqin(Scutellariae Radix)pieces were positively correlated with the contents of total flavonoids with the baicalin and wogonoside,the baicalin with wogonoside,the baicalein with wogonin(<0.01).
The processing technology of fresh cutting is feasible in the production of Huangqin(Scutellariae Radix)pieces.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |